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4,958 results

Showing: 1451 - 1500 of 4958 results

  2. pOPINMalE

    Plasmid
    #41136
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    March 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41136-plasmid-map-sequence-id-54730

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  3. pUASp YFP Rac1 CA

    Plasmid
    #52235
    Purpose
    Drosophila expression of YFP-Rac1 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Rac1 (Rac1 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  5. pOPINEneo

    Plasmid
    #53536
    Purpose
    pTriex-based expression vector which adds a C-his8 tag to the inserted gene
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 23, 2014
    Availability
    Academic Institutions and Nonprofits only
    53536-plasmid-map-sequence-id-83415

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  7. pOCC121

    Plasmid
    #118893
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease and MBP, cleavab…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118893-plasmid-map-sequence-id-232270

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  14. pOPINH

    Plasmid
    #53537
    Purpose
    pTriex-based vector with signal sequence and N-his tag for protein expression
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    53537-plasmid-map-sequence-id-83418

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  19. R-Q111R-N122D

    Plasmid
    #167178
    Purpose
    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells
    Depositor
    Susanne Dobler
    Article
    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.
    Inserts
    ATP1A1-R-Q111R-N122D
    ATP1B1
    Expression
    Bacterial and Insect
    Mutation
    Changed R at 111 to Q and changed D at 122 to N
    Promoter
    P10 and PH
    Available Since
    Feb. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    167178-plasmid-map-sequence-id-334483

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  20. pGEM-WingGFP-tan

    Plasmid
    #83473
    Purpose
    EGFP selectable marker for use in two-stage CRISPR-mediated allele replacement strategies in Drosophila. D. americana tan homology arms can be replaced with locus of choice.
    Depositor
    Patricia Wittkopp
    Article
    Lamb et al Fly (Austin). 2016 Aug 5:1-12.
    Insert
    WingGFP-tan
    Use
    CRISPR
    Expression
    Bacterial and Insect
    Promoter
    Hsp70 and enhancer from D. melanogaster yellow ge…
    Available Since
    Oct. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    83473-plasmid-map-sequence-id-159157

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  23. 892 pFastBacHTb PTEN

    Plasmid
    #10938
    Depositor
    William Sellers
    Insert
    PTEN (PTEN Human)
    Tags
    His
    Expression
    Insect
    Available Since
    Jan. 3, 2006
    Availability
    Academic Institutions and Nonprofits only

  28. pOCC70

    Plasmid
    #118878
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    Tags
    mGFP, cleavable with TEV protease; HIS6, cleavabl…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    118878-plasmid-map-sequence-id-232355

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  29. pLib6.6B

    Plasmid
    #176666
    Purpose
    Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism
    Depositor
    Norbert Perrimon
    Article
    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:
    Type
    Empty backbone
    Use
    Crispr
    Expression
    Insect
    Available Since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    176666-plasmid-map-sequence-id-348639

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  40. His-ZZ-TEV-BICDN(1-400)-GFP

    Plasmid
    #111862
    Purpose
    C-terminal GFP tag on BICDN (amino acids 1-400) for baculovirus expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8xHis-ZZ.
    Depositor
    Andrew Carter
    Article
    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do
    Insert
    BICDN(1-400)-GFP (Bicd2 Mouse)
    Tags
    8xHis tag, GFP, and ZZ tag
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    111862-plasmid-map-sequence-id-215382

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  50. pUASp YFP Cdc42 CA

    Plasmid
    #52250
    Purpose
    Drosophila expression of YFP-Cdc42 constitutively active mutant
    Depositor
    Matthew Scott
    Insert
    Cdc42 (Cdc42 Fly)
    Use
    Drosophila
    Tags
    YFP
    Expression
    Insect
    Mutation
    Q61L; CA mutation
    Promoter
    GAL4
    Available Since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1451 - 1500 of 4958 results