4,908 results

1. 908H

   Plasmid

   #160240

   Purpose

   TetO(2x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3’UTR-Ubiquitin-Renilla-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   Tet Operator (2x)
   hsp70 minimal promoter
   p-element promoter
   Firefly luciferase
   Renilla luciferase
   Ubiquitin promoter
   

   Use

   Tags

   T2A GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFAST-BAC BAF57/SMARCE1-His

   Plasmid

   #177861

   Purpose

   Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

   Depositor

   Yoshihiro Izumiya

   Article

   Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

   Insert

   SMARCE1 (SMARCE1 Human)
   

   Use

   Tags

   His tag at C-terminus with GGGGS linker

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJFRC-10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest

   Plasmid

   #217513

   Purpose

   Encodes a UAS controlled and Flp dependent conditional trangene of bicistronic myrGFP and KD Recombinase

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   10XUAS-FRT-STOP-FRT-myrGFP-2A-KDR::Pest
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLib6.4B-Consensus

   Plasmid

   #176678

   Purpose

   Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastbacHTB HA-human PP2A Cα D88N

   Plasmid

   #132633

   Purpose

   expresses HA-human PP2A Cα D88N in Hi5 insect cell

   Depositor

   Wenqing Xu

   Article

   Cho et al Nature. 2007 Jan 4;445(7123):53-7. doi:

   Insert

   PP2A Cα D88N
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

   Plasmid

   #85072

   Purpose

   Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   RhoA Biosensor constitutively active (Q63L) (RHOA Human)
   

   Use

   Tags

   6xHis and mScarlet-i and SGFP2

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Q63L

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBacI-KDAC6

   Plasmid

   #224346

   Purpose

   Expresses human KDAC6 (HDAC6) in insect cells

   Depositor

   Terry Watt

   Article

   Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

   Insert

   KDAC6 (HDAC6 Human)
   

   Use

   Tags

   TEV-cleavable His6

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Oct. 22, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

   Plasmid

   #48296

   Depositor

   Scott Gradia

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   His6 and MBP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastbac1-GST-hUSP7

   Plasmid

   #63573

   Purpose

   Expression of synthetic human USP7 tagged with GST

   Depositor

   Titia Sixma

   Article

   Faesen et al Mol Cell. 2011 Oct 7;44(1):147-59. doi:

   Insert

   USP7 (USP7 Human, Synthetic)
   

   Use

   Tags

   GST

   Expression

   Insect

   Mutation

   Synthetic based on human protein sequence (please…

   Promoter

   Polyhedrin

   Available since

   April 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSLfa-PUb-MCS

    Plasmid

    #52908

    Purpose

    Aedes aegypti polyubiquitin promoter followed by MCS

    Depositor

    Zach Adelman

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Ae aegptyi polyubiquitin

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMT-Aurora B-GFP

    Plasmid

    #24285

    Depositor

    Ron Vale

    Article

    Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

    Insert

    Aurora B Kinase (aurB Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

12. pJFRC150-20XUAS-IVS-Flp1::PEST

    Plasmid

    #32132

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    Flippase1::PEST
    

    Use

    Tags

    PEST

    Expression

    Insect

    Mutation

    Aspartic Acid at Amino Acid 5

    Promoter

    Available since

    Sept. 23, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBPhsFlp2::PEST

    Plasmid

    #63171

    Purpose

    Drosophila codon-optimized Flp2::mODC-PEST fusion gene driven from Drosophila Hsp70Bb Heat Shock Promoter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    hsFlp2::PEST
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. QUAS-jGCaMP7s-T2A-tdTomato

    Plasmid

    #200010

    Purpose

    QUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2A

    Depositor

    Carolyn McBride

    Article

    Zhao et al Nature. 2022 May;605(7911):706-712. doi:

    Insert

    3xP3-ECFP-jGCaMP7s-td-Tomato
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHACK(Gal4)-DONR(T2A-Flp1)

    Plasmid

    #194770

    Purpose

    To insert T2A-Flp1 into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-Flp1 transgene.

    Depositor

    Chun Han

    Article

    Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

    Insert

    T2A-Flp1
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p{ie1-DsRed::GW,attB}

    Plasmid

    #182173

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p{ie1-eGFP::GW,attB}

    Plasmid

    #182172

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. p{Mhc-F4-678-DsRed::GW,attB}

    Plasmid

    #182171

    Purpose

    fluorescent reporter gene with intron containing Gateway cloning cassette

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    DsRed
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcGP67A-mMD2-proA

    Plasmid

    #187878

    Purpose

    baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

    Depositor

    Bruce Beutler

    Article

    Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

    Insert

    MD2 (Ly96 Mouse)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    includes residues 19–160 of mouse MD-2

    Promoter

    Available since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

    Plasmid

    #218864

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8s
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    S26M F286Y Q315H

    Promoter

    hsp70

    Available since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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