4,932 results

1. pAc5.1B-MS2_V

   Plasmid

   #147737

   Purpose

   Insect Expression vector

   Depositor

   Elisa Izaurralde

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST8_FLAGHA_FUS_HIS

   Plasmid

   #26375

   Depositor

   Thomas Tuschl

   Article

   Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

   Insert

   FUS (FUS Human)
   

   Tags

   FLAG/HA and His

   Expression

   Insect

   Mutation

   delta 527 (STOP codon)

   Available Since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pWalium-ChtVis-Tomato

   Plasmid

   #67756

   Purpose

   Expresses a fluorescent chitin reporter under Gal4 control in Drosophila.

   Depositor

   Paul Adler

   Article

   Sobala et al Development. 2015 Nov 15;142(22):3974-81

   Insert

   dyl - chitin binding domain - tdTomato - dyl
   

   Tags

   td-tomato

   Expression

   Insect

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-1045C

   Plasmid

   #125005

   Purpose

   Expresses gRNAs targeting hid, eve, and wingless

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBPHLWL-GAL80

   Plasmid

   #26257

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Use

   Drosophila transgenesis

   Expression

   Bacterial and Insect

   Available Since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFasBac+GFP-CAMSAP3 CC+CKK

   Plasmid

   #59041

   Purpose

   for baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cells

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   CAMSAP3 CC+CKK domain (Camsap3 Mouse)
   

   Tags

   His and eGFP

   Expression

   Insect

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. R

   Plasmid

   #167170

   Purpose

   Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant in Sf9 cells

   Depositor

   Susanne Dobler

   Article

   Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

   Inserts

   ATP1A1-R
   ATP1B1
   

   Expression

   Bacterial and Insect

   Promoter

   P10 and PH

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Hy_pUbi-p63e eGFP SV40

   Plasmid

   #132650

   Purpose

   Expresses eGFP mRNA from the Drosophila Ubi-p63e promoter

   Depositor

   Jeremy Wilusz

   Article

   Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

   Insert

   eGFP
   

   Expression

   Insect

   Promoter

   Ubi-p63e

   Available Since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFastBac HTB DPP9S D808N

   Plasmid

   #169947

   Purpose

   Purification of catalytically-dead DPP9S from insect cells

   Depositor

   Hao Wu

   Article

   Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

   Insert

   DPP9 (DPP9 Human)
   

   Tags

   HIS-TEV

   Expression

   Insect

   Mutation

   D808N

   Promoter

   polyhedrin

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDW465

    Plasmid

    #8846

    Depositor

    David Waugh

    Article

    Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

    Type

    Empty backbone

    Tags

    biotin acceptor peptide

    Expression

    Insect

    Available Since

    April 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSK41

    Plasmid

    #74886

    Purpose

    template for insertion of attP site (C-terminal tag plasmid design), Puromycin-Resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    attP site
    

    Expression

    Insect

    Available Since

    April 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dll1ICD-HPC4 pBacPAK8-3

    Plasmid

    #17333

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

    Insert

    Delta-like 1 (Dll1 Mouse)
    

    Tags

    HPC4

    Expression

    Insect

    Available Since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pGS-CD64-2

    Plasmid

    #109191

    Purpose

    Encodes full-length engineered CD64 with C-terminal Twin-Strep tag to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

    Insert

    Full-length engineered CD64, derived from human CD64
    

    Tags

    Twin-Strep

    Expression

    Insect

    Mutation

    engineered for efficient expression, see publicat…

    Promoter

    Polyhedrin

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC240

    Plasmid

    #118895

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for rec. protein with N-terminal MBP tag, cleavable with 3C and C-terminal mCherry, cleavable with TEV and HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-NotI-ccdB-AscI-TEV-mCherry-3C-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and mCherry, clea…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQUAST-GAL80

    Plasmid

    #46137

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac1-OSF-TRIM5alpha(chimpanzee)

    Plasmid

    #79032

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(chimpanzee)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOET3-N-TEV-Twin-Strep

    Plasmid

    #203323

    Purpose

    Generate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cells

    Depositor

    Chiaki Murakami

    Article

    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002

    Type

    Empty backbone

    Tags

    TEV cleavable site (ENLYFQG) and Twin-strep-tag

    Expression

    Insect

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. Or1a-Gal4

    Plasmid

    #63019

    Purpose

    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4

    Depositor

    Leslie Vosshall

    Article

    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.

    Insert

    Drosophila melanogaster Or1a promoter
    

    Tags

    Drosophila ADH (aa's 1-18) fused to Yeast Ga…

    Expression

    Insect

    Available Since

    April 22, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Syt1LeftArm-3XGS-GCaMP6s-SV40-3XP3-dsRed-SV40-Syt1RightArm

    Plasmid

    #159636

    Purpose

    Donor plasmid for generating Syt1:GCaMP6s strain in Aedes aegypti with CRISPR/Cas9

    Depositor

    Carolyn McBride

    Article

    Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

    Insert

    GCaMP6s
    

    Expression

    Insect

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBacDual with LFn + FlaAAA

    Plasmid

    #84867

    Purpose

    Baculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    FlaAAA
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Mutation

    3 C-terminal leucines have been replaced by alani…

    Promoter

    Polyhedrin

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. 907H1

    Plasmid

    #160234

    Purpose

    FlightN-CymR-VP16-3X-SV40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    p-cymene repressor
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCaSpeR4-tubulin-QF#7m1

    Plasmid

    #46128

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7m1
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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23. pTriEx-mCherry-LOV2 C450A

    Plasmid

    #81031

    Purpose

    Mammalian expression of the dark state of LOV2

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV2
    

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    C450A

    Available Since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAC-Per

    Plasmid

    #33157

    Depositor

    Michael Rosbash

    Article

    Nawathean et al Mol Cell. 2004 Jan 30;13(2):213-23.

    Insert

    Per (per Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

25. Fast-GCaMP-EF05

    Plasmid

    #52558

    Purpose

    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

    Depositor

    Samuel Wang

    Article

    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

    Insert

    Fast-GCaMP-EF05
    

    Expression

    Insect

    Mutation

    changed from GCaMP3: D397N/G398A/N399D

    Available Since

    April 15, 2014

    Availability

    Academic Institutions and Nonprofits only

26. T6-pAC5.1 mEGFP-EcoRV

    Plasmid

    #206489

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Tags

    mEGFP

    Expression

    Insect

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCaSpeR4 mito roGFP2-Orp1

    Plasmid

    #65001

    Purpose

    Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Orp1 (HYR1 Budding Yeast)
    

    Tags

    roGFP2

    Expression

    Insect

    Mutation

    mitochondrial targeting sequence

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pF3BGX-TangoG-lexA

    Plasmid

    #138403

    Purpose

    RMCE vector for generating C-terminal Tango fusion

    Depositor

    Shu Kondo

    Article

    Katow et al J Neurogenet. 2019 Mar - Jun;33(2):44-51

    Insert

    TEVcs(ENLYFQG)-LexAp65
    

    Expression

    Insect

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUASTattB_NSnoFbox-vhhGFP4

    Plasmid

    #35578

    Depositor

    Markus Affolter

    Article

    Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

    Insert

    NSnoFbox-vhhGFP4 (slmb Fly)
    

    Expression

    Insect

    Mutation

    F box domain of slmb deleted.

    Available Since

    April 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFastBac(His10-Shp2)

    Plasmid

    #177899

    Purpose

    Baculo expression of His10 tag fused to human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Tags

    10xHis-TEV-SNAP tag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pAC-Cyc

    Plasmid

    #33156

    Depositor

    Michael Rosbash

    Article

    Kadener et al PLoS Biol. 2008 May 20;6(5):e119.

    Insert

    Cyc (cyc Fly)
    

    Use

    Retroviral

    Tags

    6xHIS and V5

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Dec. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid

    #169180

    Purpose

    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

    Insert

    nsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    

    Tags

    His6 (internal tag linking nsp7 and nsp8): nsp7-H…

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. IL2 F42A pAcGP67a

    Plasmid

    #41807

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Tags

    6x His

    Expression

    Insect

    Mutation

    Phenylalanine 42 to Alanine

    Promoter

    Polyhedron

    Available Since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pF3BGX-mScarlet-I

    Plasmid

    #138391

    Purpose

    RMCE vector for generating C-terminal mScarlet-I fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    mScarlet-I
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pXL-BACII-attPGAL4LWL

    Plasmid

    #26260

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Use

    Drosophila transgenesis

    Expression

    Bacterial and Insect

    Available Since

    Feb. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFastBac mPh1

    Plasmid

    #1970

    Depositor

    Robert Kingston

    Article

    Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

    Insert

    mPh1 (Phc1 Mouse)
    

    Expression

    Insect

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

37. pCaSpeR-act5cB-QF#7

    Plasmid

    #46125

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF#7
    

    Expression

    Insect

    Available Since

    Aug. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAC8_GWs-Lox (VE5589)

    Plasmid

    #163769

    Purpose

    Transfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    no insert
    

    Tags

    no tag

    Expression

    Insect

    Promoter

    PH

    Available Since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCaSpeR-act5cB-LexAQF

    Plasmid

    #61310

    Purpose

    Expresses chimeric transactivator LexA_DBD:QF2_AD in Drosophila

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA_DBD::QF2_AD
    

    Expression

    Insect

    Available Since

    Jan. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUAS-kkv-R896K::NG

    Plasmid

    #138957

    Purpose

    Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4

    Depositor

    Paul Adler

    Article

    Adler et al bioRxiv 2020

    Insert

    kkv-R896K::NG (kkv Fly)
    

    Tags

    Neon Green fused to C terminus of Kkv

    Expression

    Insect

    Mutation

    R896K

    Promoter

    UAS

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge