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pOPINHALO7

Plasmid

#41117

Depositor

Ray Owens

Article

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pMH5

Plasmid

#52531

Purpose

expresses FLP recombinase in Drosophila

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

FLP recombinase (flp Budding Yeast)

Expression

Insect

Promoter

ubiquitin

Available Since

April 23, 2014

Availability

Academic Institutions and Nonprofits only

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pAFW-Ago2

Plasmid

#50554

Purpose

Expression of FLAG tagged Ago2 in S2 cell

Depositor

Yukihide Tomari

Article

Iwasaki et al Mol Cell. 2010 Jul 30;39(2):292-9. doi:

Insert

Argonaute2 (AGO2 Fly)

Tags

FLAG

Expression

Insect

Promoter

Actin5C

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only

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ptubP-QS

Plasmid

#24352

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QS

Tags

white gene

Expression

Insect

Available Since

June 1, 2010

Availability

Academic Institutions and Nonprofits only

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pLib6.4B-Consensus

Plasmid

#176678

Purpose

Expression of sgRNA under mosquito consensus U6 promoter & delivery of D.mel-Actin5C::EBFP2-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-Rgfp)

Plasmid

#55221

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

StrepII and msfGFP

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8s-p10

Plasmid

#218864

Purpose

Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

Depositor

GENIE Project

Article

Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

Insert

RSET-jGCaMP8s

Tags

6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

Expression

Insect

Mutation

S26M F286Y Q315H

Promoter

hsp70

Available Since

May 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCFD7

Plasmid

#73916

Purpose

gRNA vector for AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

tRNA:hAsCpf1-gRNA:tRNA

Use

CRISPR

Expression

Insect

Promoter

dU6:3

Available Since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pattB-QF-hsp70

Plasmid

#24368

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

QF

Expression

Insect

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

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pUAST>stop>mCD8-GFP

Plasmid

#24385

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

mCD8-GFP

Expression

Insect

Available Since

May 5, 2010

Availability

Academic Institutions and Nonprofits only

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pUASattB-ERK-SPARK

Plasmid

#106923

Purpose

Express ERK-SPARK, an activity reporter of ERK in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

ERK-SPARK

Expression

Insect

Available Since

March 15, 2018

Availability

Academic Institutions and Nonprofits only

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pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

Plasmid

#32139

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

B3RT> STOP STOP B3RT>

Expression

Insect

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pGL3-Gateway-DSCP

Plasmid

#71506

Purpose

flySTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the DSCP core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Flystarr-seq validation vector

Tags

luciferase

Expression

Insect

Available Since

Dec. 29, 2015

Availability

Academic Institutions and Nonprofits only

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doraWT_rescue_construct

Plasmid

#190609

Purpose

Puromycin-selectable expression of Dora (CG34401) in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

Dorado (CG34401 Fly)

Expression

Insect

Promoter

Actin-5c

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pUASp YFP Rab5 WT

Plasmid

#53468

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab05 (Rab5 Fly)

Tags

YFP

Expression

Insect

Promoter

GAL4

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
pFBNb-cALFA

Plasmid

#159990

Purpose

For the protein expression with ALFA tag at C-terminus in insect cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Insect

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pDEST-HemmarG

Plasmid

#31221

Depositor

Yuh-Nung Jan

Article

Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

Insert

CD4-tdGFP (CD4 Fly, Human, Aequorea victoria)

Tags

CD4 and EGFP/GFP

Expression

Insect

Mutation

Fusion of signal peptide of Drosophila Akh gene, …

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pUASattB-PKA-SPARK

Plasmid

#106922

Purpose

Express PKA-SPARK, an activity reporter of protein kinase A in Drosophila

Depositor

Xiaokun Shu

Article

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Insert

PKA-SPARK

Expression

Insect

Available Since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

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vas-Cas9.874Z

Plasmid

#112687

Purpose

Express hSpCas9 under vasa promoter

Depositor

Omar Akbari

Article

Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

Insert

hSpCas9

Tags

3x FLAG, EGFP, and NLS

Expression

Insect

Mutation

Humanized Cas9 bacterial sequence

Promoter

vasa

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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