4,908 results

1. pOCC154

   Plasmid

   #118902

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal TwinStrep, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-NotI-ccdB-AscI-3C-TwinStrep-stop-HindIII cassette
   

   Use

   Tags

   TwinStrep, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AlphaC

   Plasmid

   #124525

   Purpose

   pColE1_AlphaC

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   June 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSTARR-seq_fly-hsp70

   Plasmid

   #71500

   Purpose

   flySTARR-seq screening vector with hsp70 core promoter

   Depositor

   Alexander Stark

   Article

   Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

   Type

   Empty backbone

   Use

   Flystarr-seq screening vector

   Tags

   sgGFP (SuperGlo, Qbiogene, Inc)

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 438-GST

   Plasmid

   #97092

   Purpose

   GST-tev-yORF

   Depositor

   Chris Jeans

   Article

   Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   GST tag and TEV cleavage site

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin promoter

   Available since

   July 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pattB-QF-hsp70

   Plasmid

   #24368

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   QF
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AT4G23740 J11_pECIA2

   Plasmid

   #114998

   Purpose

   Bait vector AT4G23740 J11_pECIA2 should be used with prey vector AT4G23740 J11_pECIA14.

   Depositor

   Youssef Belkhadir

   Article

   Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

   Insert

   AT4G23740 (AT4G23740 Mustard Weed)
   

   Use

   Tags

   Fc, V5 and hexahistidine tags

   Expression

   Insect

   Mutation

   Promoter

   Metallothionein (Copper-Inducible)

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAC-CR7T-gRNA2.1-nlsBFP

   Plasmid

   #170515

   Purpose

   gRNA cloning vector containing a CR7T promoter, gRNA(2.1) scaffold, and a Ubi-mTagBFP-NLS marker; optimized for germline CRISPR.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   CR7T

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUAST>stop>mCD8-GFP

   Plasmid

   #24385

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   mCD8-GFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJFRC160-21XUAS-B3RT>-dSTOP-B3RT>-myr::RFP

   Plasmid

   #32139

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   B3RT> STOP STOP B3RT>
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGP-JFRC59-13XLexAop2-IVS-Syn21-RSET-jGCaMP8f-p10

    Plasmid

    #218867

    Purpose

    LexA activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8f
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    Q315L

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBac{Act88F-eGFP,attB,w+}

    Plasmid

    #182198

    Purpose

    EGFP driven by Act88F enhancer

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST-HemmarG

    Plasmid

    #31221

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Insert

    CD4-tdGFP (CD4 Human, Aequorea victoria, Fly)
    

    Use

    Tags

    CD4 and EGFP/GFP

    Expression

    Insect

    Mutation

    Fusion of signal peptide of Drosophila Akh gene, …

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p(bhsp68-Cas9)

    Plasmid

    #65959

    Purpose

    Tribolium basal heat shock promoter driving Cas9

    Depositor

    Michalis Averof

    Article

    Gilles et al Development. 2015 Jul 9. pii: dev.125054

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    nuclear localization sequence

    Expression

    Insect

    Mutation

    Promoter

    bhsp

    Available since

    Aug. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLib6.4-Aalb_743

    Plasmid

    #176662

    Purpose

    Expression of sgRNA under Ae. albopictus U6 (AALF029743) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Type

    Empty backbone

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. TH0901-pOCC177-FUS-wt_opt(Nhe)

    Plasmid

    #221885

    Purpose

    Shuttle vector for baculovirus production, using FlashBac bacmid

    Depositor

    Anthony Hyman

    Article

    Wang et al Cell. 2018 Jul 26;174(3):688-699.e16. do

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    6xHis-MBP-PreScission and TEV-SNAP-tag

    Expression

    Insect

    Mutation

    wt

    Promoter

    PH promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKSB-618 U6-sgRNA2/3 GGAA AACA

    Plasmid

    #173674

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBPGAL80Uw-4

    Plasmid

    #26235

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL80 (GAL80 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac (GST-SNAP-Shp2)

    Plasmid

    #177897

    Purpose

    Baculo expression of GST tag fused to SNAP tag and human Shp2

    Depositor

    Ron Vale

    Article

    Hui et al Science. 2017 Mar 31;355(6332):1428-1433

    Insert

    Shp2 (PTPN11 Human)
    

    Use

    Tags

    TEV-GST-PreScission cut site-SNAP tag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmAtx2_AE

    Plasmid

    #148548

    Purpose

    Insect Expression of DmAtx2

    Depositor

    Elisa Izaurralde

    Insert

    DmAtx2 (Atx2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Three silent mutations and two deletions compared…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. IL2 pAcGP67a

    Plasmid

    #41806

    Depositor

    Chris Garcia

    Article

    Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

    Insert

    IL2 (IL2 Human)
    

    Use

    Tags

    6x His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedron

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only