4,908 results

1. IMPT-2070

   Plasmid

   #172092

   Purpose

   pFastBac1 vector with GP64 promoter replacing polH promoter expressing A2aAR+BRIL GPCR

   Depositor

   Raymond Stevens

   Article

   Liu et al Science. 2012 Jul 13;337(6091):232-6. do

   Insert

   Homo sapiens adenosine A2a receptor (ADORA2A) (ADORA2A Human)
   

   Use

   Tags

   cytochrome b562RIL

   Expression

   Insect

   Mutation

   Promoter

   gp64

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. QPRTA-c002

   Plasmid

   #205115

   Purpose

   QPRT protein expression

   Depositor

   Paul Brennan

   Insert

   QPRT (QPRT Human)
   

   Use

   Tags

   TEV-6His

   Expression

   Insect

   Mutation

   WT

   Promoter

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. p(U6b-BsaI-gRNA)

   Plasmid

   #65956

   Purpose

   Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

   Depositor

   Michalis Averof

   Article

   Gilles et al Development. 2015 Jul 9. pii: dev.125054

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Aug. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQUAST-mCD8-GFP

   Plasmid

   #24351

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   CD8-GFP-SV40
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAC-DsRed-QF2-hsp70

   Plasmid

   #104876

   Purpose

   General purpose vector for cloning enhancer/promoter regions to drive QF2 expression; used for mosquito

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

   Insert

   QF2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAC-dCLK

   Plasmid

   #33153

   Depositor

   Michael Rosbash

   Article

   McDonald et al Mol Cell Biol. 2001 Feb;21(4):1207-17.

   Insert

   CLK (Clk Fly)
   

   Use

   Retroviral

   Tags

   6xHIS and V5

   Expression

   Insect

   Mutation

   Promoter

   Actin 5C

   Available since

   Jan. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

   Plasmid

   #32145

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   loxP> STOP STOP loxP>
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pIE-TGFB2-H6

   Plasmid

   #128500

   Purpose

   Expresses human pro-TGFβ2 (from L21 to S414) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   TGFB2 (TGFB2 Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Insect

   Mutation

   Promoter

   IE1

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBIG1b_10xHisTEVATG5_ATG12

   Plasmid

   #190861

   Purpose

   Plasmid for the expression of ATG5 and ATG12 protein subunit complexes. Internal References: SMC1178

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Inserts

   ATG5 (ATG5 Human)
   ATG12 (ATG12 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ReactionC_pBIG1a_ATG7_ATG10

    Plasmid

    #190860

    Purpose

    Plasmid for the expression and purification of ATG7 and ATG10 protein subunit complexes. Internal reference: SMC1179

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG7 (ATG7 Human)
    ATG10 (ATG10 )
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTriEx-NTOM20-mVenus-Zdk3

    Plasmid

    #81012

    Purpose

    Expression of Zdk1 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk3
    

    Use

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Dynein-2_complex

    Plasmid

    #132536

    Purpose

    Full length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.

    Depositor

    Andrew Carter

    Article

    Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

    Inserts

    DYNC2H1 (DYNC2H1 Human)
    DYNC2LI1 (DYNC2LI1 Human)
    WDR34 (WDR34 Human)
    WDR60 (WDR60 Human)
    DYNLL1 (DYNLL1 Human)
    DYNLL2 (DYNLL2 Human)
    DYNLRB1 (DYNLRB1 )
    DYNLRB2 (DYNLRB2 Human)
    DYNLT1 (DYNLT1 Human)
    TCTEX1D2 (TCTEX1D2 Human)
    DYNLT3 (DYNLT3 Human)
    LC8-like (LOC392067 Human)
    

    Use

    Tags

    8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …

    Expression

    Insect

    Mutation

    Codon optimised for Sf9 expression

    Promoter

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC153

    Plasmid

    #118901

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal TwinStrep, cleavable with 3C

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-TwinStrep-3C-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    TwinStrep, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKM50

    Plasmid

    #123656

    Purpose

    Encodes Renilla luciferase under the Aedes aegypti ubiquitin UbL40 promoter for constitutive expression in mosquito cells

    Depositor

    Kevin Maringer

    Article

    Fredericks et al PLoS Negl Trop Dis. 2019 Nov 6;13(11):e0

    Insert

    Aedes aegypti UbL40 promoter
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UbL40 promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pOCC102-MBP-eIF4G

    Plasmid

    #219971

    Purpose

    eIF4G protein purification

    Depositor

    Simon Alberti

    Insert

    eIF4G (TIF4631 Budding Yeast)
    

    Use

    Tags

    6xHis-MBP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pQUASp-mCD8GFP

    Plasmid

    #46163

    Purpose

    Expression of murine CD8GFP in insects

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Insert

    mCD8GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRB17

    Plasmid

    #52527

    Purpose

    serves as template for PCR-mediated fusion of U6-promoter with sgRNA

    Depositor

    Klaus Foerstemann

    Article

    Bottcher et al Nucleic Acids Res. 2014 Apr 19.

    Insert

    Drosophila U6C promoter
    

    Use

    CRISPR; Blunt cloning kit

    Tags

    T7-promoter

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFastBacI-KDAC8 H143A

    Plasmid

    #224344

    Purpose

    Expresses human KDAC8 (HDAC8) H143A in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Protein Expr Purif. 2018 Jan;141:1-6. do

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Histidine 143 mutated to alanine

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pFastBacI-KDAC8 D101E

    Plasmid

    #224350

    Purpose

    Expresses human KDAC8 (HDAC8) D101E in insect cells

    Depositor

    Terry Watt

    Article

    Toro et al Biochemistry. 2021 Aug 24;60(33):2524-25

    Insert

    KDAC8 (HDAC8 Human)
    

    Use

    Tags

    TEV-cleavable His6

    Expression

    Insect

    Mutation

    Aspartate 101 mutated to glutamate

    Promoter

    Polyhedrin

    Available since

    Oct. 22, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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