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pUAST_NSlmb-vhhGFP4

Plasmid

#35575

Depositor

Markus Affolter

Article

Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

Insert

NSlmb-vhhGFP4 (slmb Fly)

Expression

Insect

Available Since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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pFAST-BAC BAF57/SMARCE1-His

Plasmid

#177861

Purpose

Transfer vector to generate recombinant baculovirus expressing BAF57/SMARCE1-His protein

Depositor

Yoshihiro Izumiya

Article

Shimoda et al Commun Biol. 2021 Dec 2;4(1):1330. doi:

Insert

SMARCE1 (SMARCE1 Human)

Tags

His tag at C-terminus with GGGGS linker

Expression

Insect

Promoter

polyhedrin

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pAct-GW-HygroR

Plasmid

#149610

Purpose

Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

Gateway cassette (ccdb + Chlor.R.)

Expression

Insect

Promoter

Act5c

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pOmnibac_ZZ_linker_ybbR_Tau

Plasmid

#159717

Purpose

Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

Depositor

Ahmet Yildiz

Article

Ferro et al Science. 2022 Jan 21;375(6578):326-331.

Insert

Microtubule-associated protein tau (MAPT Human)

Tags

ZZ affinity tag, ybbR tag

Expression

Insect

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIEx4-Dio-GFP

Plasmid

#198278

Purpose

Cre reporter for insect cells; double-floxxed-EGFP

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

pIEx4-Dio-GFP

Expression

Insect

Promoter

IE1 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pOPINE-3C-YFP

Plasmid

#214028

Purpose

Expression of fluorescent fusion proteins

Depositor

Ray Owens

Insert

YFP

Tags

Hexahistidine

Expression

Bacterial, Insect, and Mamm…

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pScarlessHD-C-3x127D01-DsRed

Plasmid

#171578

Purpose

For scarless insertion of 3x127D01 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

Depositor

Norbert Perrimon, Hidde Ploegh

Article

Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

Insert

3x127D01 (CXCR2 Human)

Expression

Insect

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBPGAL80Uw-4

Plasmid

#26235

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

Insert

GAL80 (GAL80 Budding Yeast)

Expression

Insect

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pENTR R4 ATCC LacZ GCTT

Plasmid

#173668

Purpose

Destination vector for Golden Gate Cloning assemblies, blue-white screening

Depositor

Eric Marois

Article

Green et al Elife. 2023 Dec 5;12:e93142. doi: 10.755

Type

Empty backbone

Expression

Bacterial and Insect

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pE5c

Plasmid

#17465

Purpose

modified pENTR vector for C-terminal ECFP tag fusion with your gene of interest

Depositor

Giovanna Benvenuto

Article

Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

Type

Empty backbone

Use

Modified gateway entry vector

Tags

ECFP

Expression

Bacterial, Insect, Mammalia…

Available Since

March 21, 2008

Availability

Academic Institutions and Nonprofits only

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NHR1+2-Flag pBacPAK8-3

Plasmid

#17324

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pQUAST-mCD8-GFP

Plasmid

#24351

Depositor

Liqun Luo

Article

Potter et al Cell. 2010 Apr 30. 141(3):536-48.

Insert

CD8-GFP-SV40

Expression

Insect

Available Since

April 30, 2010

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)

Plasmid

#79034

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human) (TRIM5 Human)

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

polyhedrin

Available Since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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pJFRC172-10XUAS-loxP>-dSTOP-loxP>-myr::GFP

Plasmid

#32145

Depositor

Gerald Rubin

Article

Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

Insert

loxP> STOP STOP loxP>

Expression

Insect

Available Since

Sept. 23, 2011

Availability

Academic Institutions and Nonprofits only

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438-GST

Plasmid

#97092

Purpose

GST-tev-yORF

Depositor

Chris Jeans

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

GST tag and TEV cleavage site

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pOPINMSYB

Plasmid

#41118

Depositor

Ray Owens

Article

Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

Type

Empty backbone

Expression

Bacterial, Insect, and Mamm…

Available Since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pBIG1a_nsp12-His6-3xFlag (SARS-CoV-2)

Plasmid

#169182

Purpose

Baculoviral transfer vector to express nsp12-His6-3xFlag (SARS-CoV-2) in insect cells

Depositor

John Diffley

Article

Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443

Insert

nsp12-His6-3xFlag (ORF1ab Synthetic, SARS-CoV-2)

Tags

His6-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pRB17

Plasmid

#52527

Purpose

serves as template for PCR-mediated fusion of U6-promoter with sgRNA

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

Drosophila U6C promoter

Use

CRISPR; Blunt cloning kit

Tags

T7-promoter

Expression

Insect

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pPac Sp1

Plasmid

#12095

Depositor

Robert Tjian

Article

Courey et al Cell. 1988 Dec 2. 55(5):887-98.

Insert

Sp1 (SP1 Human)

Expression

Insect

Available Since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only