4,932 results

1. WDR34

   Plasmid

   #132526

   Purpose

   Human WDR34, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   WDR34 (WDR34 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. TCTEX1D2

   Plasmid

   #132529

   Purpose

   Human TCTEC-1D2, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   TXCTEX1D2 (TCTEX1D2 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. DYNC2LI1

   Plasmid

   #132527

   Purpose

   Human LIC3, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNC2LI1 (DYNC2LI1 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. WDR60

   Plasmid

   #132525

   Purpose

   Human WDR60, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   WDR60 (WDR60 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. DYNLRB1

   Plasmid

   #132533

   Purpose

   Human Rbl-1, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLRB1 (DYNLRB1 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. DYNLT1

   Plasmid

   #132528

   Purpose

   Human TCTEX-1, codon optimised for Sf9 expression.

   Depositor

   Andrew Carter

   Article

   Toropova et al Nat Struct Mol Biol. 2019 Aug 26. pii: 1

   Insert

   DYNLT1 (DYNLT1 Human)
   

   Expression

   Insect

   Mutation

   Codon optimised for Sf9 expression

   Available Since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-GFP-SAK

   Plasmid

   #31755

   Depositor

   Ron Vale

   Article

   Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

   Insert

   Sak (SAK Fly)
   

   Tags

   GFP

   Expression

   Insect

   Mutation

   A115S

   Promoter

   Metallothionein

   Available Since

   Jan. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pSGE_768_Nhe2-EcR_dCP

   Plasmid

   #71223

   Purpose

   Luciferase reporter

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   768_Nhe2-EcR_dCP
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Promoter

   DSCP Drosophila synthetic core promoter

   Available Since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSGE_769_DipB-EcR_dCP

   Plasmid

   #71222

   Purpose

   Luciferase reporter

   Depositor

   Alexander Stark

   Article

   Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

   Insert

   769_DipB-EcR_dCP
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Insect

   Promoter

   DSCP Drosophila synthetic core promoter

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBIG2ab_nsp14/nsp10-6His-3xFlag (SARS-CoV-2)

    Plasmid

    #169164

    Purpose

    Baculoviral transfer vector to co-express SARS-CoV-2 nsp14 and nsp10 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp14/nsp10-6His-3xFlag (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    6His-3xFlag (nsp10 C-terminus)

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAc5.1B-EGFP-DmNot1_J

    Plasmid

    #146709

    Purpose

    Insect Expression of DmNot1

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmNot1 (Not1 Fly)
    

    Expression

    Insect

    Mutation

    Three non silent mutations N605S, K759M, G1999S a…

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. FLAGHis10pFastBacHTA/Serca1

    Plasmid

    #74401

    Purpose

    expression of SERCA in insect cells

    Depositor

    Robert Nakamoto

    Insert

    SERCA1 (ATP2A1 rabbit)
    

    Tags

    10His and FLAG

    Expression

    Insect

    Mutation

    NcoI site destroyed (silent) T898

    Promoter

    polyhedrin

    Available Since

    April 6, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCaSpeR4 cyto Grx1-roGFP2

    Plasmid

    #64999

    Purpose

    Expression of cytosolic Grx1-roGFP2 under the alphaTub84B promoter

    Depositor

    Tobias Dick

    Article

    Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

    Insert

    Glutaredoxin-1 (GLRX Human)
    

    Tags

    roGFP2

    Expression

    Insect

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pACEBac1-NEDD1

    Plasmid

    #178073

    Purpose

    Insect cell expression of NEDD1

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Insert

    NEDD1 (NEDD1 Human)
    

    Expression

    Insect

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUAST tdTomato mouse Rab35 WT

    Plasmid

    #53514

    Depositor

    Matthew Scott

    Article

    Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

    Insert

    Rab35 (Rab35 Mouse)
    

    Tags

    tdtomato

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    June 3, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pMT-HA-STX4

    Plasmid

    #89311

    Purpose

    Expresses HA tagged syntaxin 4 in Drosophila cells

    Depositor

    Andrew Peden

    Insert

    STX4 (Syx4 Fly)
    

    Tags

    HA tag

    Expression

    Insect

    Promoter

    P-MT (Metallothionein)

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. S

    Plasmid

    #167172

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S
    ATP1B1
    

    Expression

    Bacterial and Insect

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBC2FG

    Plasmid

    #31281

    Depositor

    Aziz Sancar

    Article

    Ye et al J Biol Chem. 2011 Jul 22;286(29):25891-9

    Insert

    Cry2 (Cry2 Mouse)
    

    Tags

    Flag and His

    Expression

    Insect

    Available Since

    Sept. 22, 2011

    Availability

    Academic Institutions and Nonprofits only

20. pAC-luc-TimRA

    Plasmid

    #33133

    Depositor

    Michael Rosbash

    Article

    Kadener et al Genes Dev. 2009 Sep 15;23(18):2179-91. E

    Insert

    Tim-RA 3' UTR (tim Fly)
    

    Use

    Luciferase

    Tags

    Firefly Luciferase

    Expression

    Insect

    Promoter

    Actin 5C

    Available Since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

21. pJFRC7-RFPnls-PQRv2-mCD8:GFP

    Plasmid

    #73974

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in insect cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP
    RFP
    

    Tags

    mCD8 and nls

    Expression

    Insect

    Promoter

    20XUAS-HS

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. FAK

    Plasmid

    #186141

    Purpose

    For expresseion of recombinant FAK using baccolovirus in SF9 cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PTK2 (PTK2 Mouse)
    

    Tags

    His6

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 59 pLIB_HIS_HA

    Plasmid

    #160308

    Purpose

    N-term 3C cleavable His-HA tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    12xHis and 3xHA

    Expression

    Insect

    Promoter

    polyhydrin

    Available Since

    Jan. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUAST-FOXO 3A

    Plasmid

    #52983

    Purpose

    insect expression of FOXO triple mutant

    Depositor

    Marc Montminy, Biao Wang

    Insert

    FOXO (foxo Fly)
    

    Tags

    3x HA

    Expression

    Insect

    Mutation

    3A, triple mutant, T44A S190A S259A

    Promoter

    Hsp70

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

25. pFastBac GST-STIL

    Plasmid

    #80265

    Purpose

    baculovirus creation plasmid for GST-STIL

    Depositor

    Andrew Holland

    Article

    Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

    Insert

    STIL (STIL Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    July 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBac[3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP]

    Plasmid

    #86455

    Purpose

    Nuclear reporter (histone-tagged EGFP) construct with Tribolium specific polyubiquitin promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)

    Depositor

    Gregor Bucher

    Insert

    3xP3-gTc'v;Tc'puP-Tc'H2Av_EGFP
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSGE_763_4xUAS_UPS_hkCP

    Plasmid

    #71171

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    763_4xUAS_UPS_hkCP
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Promoter

    DSCP Drosophila synthetic core promoter

    Available Since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFasBac+GFP-CAMSAP2 CC+CKK

    Plasmid

    #59039

    Purpose

    for baculovirus expression of CAMSAP2 CC+CKK in Sf9 cells

    Depositor

    Ron Vale

    Article

    Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

    Insert

    CAMSAP2 CC+CKK domains (CAMSAP2 Human)
    

    Tags

    His and eGFP

    Expression

    Insect

    Available Since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBac[3xP3 g Tc' v]

    Plasmid

    #86446

    Purpose

    pBAc transposon with transformation marker for Tribolium transgenesis; Rescues vermilion white eyed mutant to black eyes; (transformation plasmid)

    Depositor

    Gregor Bucher

    Insert

    3xP3 g Tc' v
    

    Expression

    Insect

    Available Since

    March 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pUASP dsRed Rab11 DN

    Plasmid

    #44831

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab11 (Rab11 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    S25N (dominant negative)

    Available Since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

31. DPP7 (3JYH)

    Plasmid

    #25354

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    dpp07.029.492 (DPP7 Human)
    

    Tags

    His

    Expression

    Insect

    Available Since

    Aug. 19, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

32. p5xUAS

    Plasmid

    #165891

    Purpose

    Synthetic 5xUAS GAL4 driver response element in enhancer grammar, requires minimal promoter for assembly

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    5xUAS
    

    Expression

    Insect

    Available Since

    Oct. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGAL80

    Plasmid

    #165825

    Purpose

    GAL4 inhibitor coding sequence (CDS)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    GAL80
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pQS

    Plasmid

    #165826

    Purpose

    QF inhibitor coding sequence (CDS)

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    QS
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. p5xQUAS

    Plasmid

    #165818

    Purpose

    Synthetic 5xQUAS QF driver response element in enhancer grammar, requires minimal promoter for assembly

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    5xQUAS
    

    Expression

    Insect

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pQUAST>stop>shibire (ts1)

    Plasmid

    #24363

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Shibire (ts1) (shi Fly)
    

    Expression

    Insect

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pMT-DREDD-V5-His

    Plasmid

    #63749

    Purpose

    Expresses drosophila DREDD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

    Depositor

    Cyril Gueydan

    Insert

    Death related ced-3/Nedd2-like caspase isoform E (Dredd Fly)
    

    Tags

    V5-6XHis

    Expression

    Insect

    Promoter

    pMT

    Available Since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAFM-NbrWT

    Plasmid

    #40830

    Purpose

    Expresses Flag, Myc tagged Nbr in Drosophila cells

    Depositor

    Phillip Zamore

    Article

    Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

    Insert

    Nbr (Nbr Fly)
    

    Tags

    3xFLAG and 6xMyc

    Expression

    Insect

    Promoter

    Actin5C

    Available Since

    Sept. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

39. pAc5.1B-lambdaN-HA-DmBicaudalC_P

    Plasmid

    #147163

    Purpose

    Insect Expression of DmBicaudalC

    Depositor

    Elisa Izaurralde

    Insert

    DmBicaudalC (BicC Fly)
    

    Expression

    Insect

    Mutation

    12 silent and three non silent mutattions I331T, …

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. No-repeats

    Plasmid

    #122487

    Purpose

    A control plasmid that is similar to PRExpress but lacks poly-PRE sequence. (GenBank: MF058599.1)

    Depositor

    Renato Paro

    Article

    Akmammedov et al Sci Rep. 2017 Jul 31;7(1):6899. doi: 10.

    Insert

    LacZ
    

    Expression

    Insect

    Available Since

    March 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge