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Plasmid#52246PurposeDrosophila expression of YFP-Ras85D dominant negative mutantDepositorInsertRas85D (Ras85D Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS17N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pTriEx-mVenus-Zdk1-RhoA Q63L
Plasmid#81046Purposemammalian expression of Zdk1 tagged RhoA Q63LDepositorInsertsUseTagsmVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRVV655
Plasmid#119019PurposeDrives TP901-1 recombinase expression in the D. melanogaster germ lineDepositorInsertTP901-1 recombinase
UseTagsSV40 NLSExpressionInsectMutationPromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427V
Plasmid#81044Purposemammalian expression of LOV2 I427VDepositorInsertLOV I427V
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427VPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LINXa3
Plasmid#73614PurposeLight-induced nuclear export switch for microscopy in mammalian cells.DepositorInsertLINXa3
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationPromoterChicken B-ActinAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAC-QS
Plasmid#24341DepositorInsertQS
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 31, 2010AvailabilityAcademic Institutions and Nonprofits only -
pScarlessHD-N-3x127D01-DsRed
Plasmid#171579PurposeFor scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.DepositorInsert3x127D01 (CXCR2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTubulin NLS-mCherry-LEXY
Plasmid#182597PurposeDrosophila integration plasmid expressing NLS-mCherry-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertmCherry
UseTagsLEXY and NLSExpressionInsectMutationPromoterAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-PE2-attB
Plasmid#149550PurposeExpression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.DepositorInsertPE2
UseCRISPRTagsExpressionInsectMutationPromoterUASAvailable sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS ChrimsonR mCherry_tr
Plasmid#111548PurposeOptogenetics in Drosophila: LexAop2-induced expression of ChrimsonR mCherryDepositorInsertChrimsonR_mCherry_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAEL005635-Cas9
Plasmid#100608PurposeThe promoter of AAEL005635 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegyptiDepositorInsertsAAEL005635-hspcas9-GFP
Opie2-dsRed
UseCRISPRTagsGFP and dsREDExpressionInsectMutationPromoterAAEL005635 and Opie2Available sinceOct. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B DN
Plasmid#52243PurposeDrosophila expression of YFP-Ras64B dominant negative mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationS19N; DN mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B CA
Plasmid#52244PurposeDrosophila expression of YFP-Ras64B constitutively active mutantDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ63L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Ras64B WT
Plasmid#52245PurposeDrosophila expression of YFP-Ras64BDepositorInsertRas64B (Ras64B Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceMarch 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pUb dRxCas13d + RxCas13d guide RNA
Plasmid#176304PurposeExpresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cellsDepositorInsertRxCas13d
UseTagsHA tagExpressionInsectMutationR239A, H244A, R858A, H863APromoterUbi-p63eAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOmnibac_ZZ_linker_K560_GFP_SNAP
Plasmid#159720PurposeExpresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tagDepositorInsertKIF5B (only amino acids 1-560), K560 (KIF5B Human)
UseTagsGFP and SNAP tags and ZZ affinity tagExpressionInsectMutationOnly amino acids 1-560PromoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
56 pLIB_CBP
Plasmid#160305PurposeN-term 3C cleavable CBP tag for insect cell expressionDepositorTypeEmpty backboneUseTagsCBPExpressionInsectMutationPromoterpolyhydrinAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only