4,908 results
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Plasmid#173671PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only
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pACgp67B-Her2mP
Plasmid#10793DepositorInsertErbB2 ScFv (ERBB2 Human)
UseTags6His and Truncated ProtamineExpressionInsectMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1-VAV2 DH/PH/C1
Plasmid#81133Purposemammalian expression of Zdk1 tagged VAV2 DH/PH/C1 domainDepositorInsertsUseTagsmVenusExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDest-636
Plasmid#159574PurposeGateway Destination vector for protein expression in baculovirusDepositorTypeEmpty backboneUseTagsHis6-MBPExpressionInsectMutationPromoterAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-RLuc-A90-HhR_O
Plasmid#147073PurposeInsect Expression of A90-HhRDepositorInsertA90-HhR
UseTagsExpressionInsectMutationA127 instead of A90 (see comments below)PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pActin5c-CD-dCas9m4-UDI-attB
Plasmid#104879PurposeTo generate point mutations using CRISPR/Cas9 system; encoded cytidine deaminase fused to deactived Cas9DepositorInsertCD-dCas9(m4)-UDI
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + Needle
Plasmid#84868PurposeBaculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
Needle
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.EPOP
Plasmid#125170PurposeExpresses human EPOP in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertEPOP (EPOP Human)
UseTagsHis-MBPExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.EED
Plasmid#125174PurposeExpresses human EED in insect cells, under a TEV-cleavable N-terminal polyhistidine tag.DepositorInsertPolycomb protein EED (EED Human)
UseTagsHis-TEVExpressionInsectMutationPromoterPolyhedrinAvailable sinceMay 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-p65-3xP3-RFP
Plasmid#127565PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-p65-SV40 and 3xP3-RFP visible eye marker.DepositorInsertp65
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-DsRedSV40 GGGG AAGA
Plasmid#174532Purpose3xP3-DsRed fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-DsRed BsaI cassette
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKSB- 3xP3-mTurqSV40 GGGG AAGA
Plasmid#174533Purpose3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoesDepositorInsert3xP3-mTurquoise BsaI cassette
UseTagsExpressionInsectMutationPromoter3xP3Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBDP
Plasmid#17566DepositorInsertmini w+
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
AlphaB
Plasmid#124524PurposepColE1_AlphaBDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
AlphaD
Plasmid#124526PurposepColE1_AlphaDDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pattB-synaptobrevin-GAL4-hsp70
Plasmid#46107DepositorInsertGAL4
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBig1a zz TEV YBBR TPP1 MBP TEV TIN2
Plasmid#185446PurposeCoexpresses human TPP1 with a zz affinity tag, YBBR labeling site, and TEV cleavage site and human TIN2 with an MBP affinity tag and TEV cleavage site in insect cellsDepositorUseTagsMBP, YBBR, and ZZExpressionInsectMutationPromoterAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDest-623 (attR4-attR3)
Plasmid#161878PurposeMultisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell systemDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST-GFP-RpL10Ab
Plasmid#71729PurposeExpression of Drosophila melanogaster Rpl10Ab-GFP fusionDepositorInsertRpL10Ab (RpL10Ab Fly)
UseTagsEGFPExpressionInsectMutationPromoterhsp70Available sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only