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Plasmid#183903Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAedes aegypti PUbAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX3
Plasmid#183904Purposedonor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locusDepositorInsertGFP
UseTagsExpressionInsectMutationPromoterAedes aegypti PolyubiquitinAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL80
Plasmid#138396PurposeRMCE vector for generating C-terminal T2A-GAL80 fusionDepositorInsertT2A-GAL80
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-scFv
Plasmid#78900PurposeExpresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cellsDepositorInsertscFV-VP64
UseCRISPRTagsGFPExpressionInsectMutationPromoterpActin (Drosophila)Available sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pGB-08-09-BECN1
Plasmid#187991PurposePlasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325DepositorInsertBECN1 (BECN1 Human)
UseTagsExpressionBacterial and InsectMutationPromoterAvailable sinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUAST-HA-dAtg13
Plasmid#111802PurposeInsect Expression of dAtg13DepositorInsertdAtg13 (Atg13 Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H
Plasmid#146503PurposeInsect Expression of DmDCP1_327-372DepositorInsertDmDCP1_327-372 (Dcp1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIE-C-hα2M-H6
Plasmid#128494PurposeExpresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.DepositorInsertA2M (R787-N1473) (A2M Human)
UseTagsHis tag (6x)ExpressionInsectMutationcontains R787-N1473PromoterIE1Available sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMT-GFP-p150Dynactin
Plasmid#24283DepositorInsertp150 Dynactin (DCTN1-p150 Fly)
UseTagsGFPExpressionInsectMutationPromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
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pGS-FCER1G-1
Plasmid#109192PurposeEncodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression systemDepositorInsertHuman full-length FCER1G, codon-optimized (FCER1G Human)
UseTagsExpressionInsectMutationPromoterp10Available sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-QtgRNA2.1-8R
Plasmid#170514PurposegRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.DepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterU6:3Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOCC121
Plasmid#118893Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3CDepositorInsertNcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac FPH
Plasmid#1925DepositorUseTagsFlagExpressionInsectMutationPromoterAvailable sinceAug. 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pFastBacDual with LFn + PrgJ
Plasmid#84869PurposeBaculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel proteinDepositorInsertsLFn
PrgJ
UseBaculovirusTags6xHisExpressionInsectMutationPromoterPolyhedrinAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP
Plasmid#32135DepositorInsertB2RT> STOP STOP B2RT>
UseTagsExpressionInsectMutationPromoterAvailable sinceNov. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAc-His-zfCsf1a
Plasmid#168103PurposeExpress soluble His-tagged zebrafish Csf1a in insect cellsDepositorInsertCsf1a (csf1a Zebrafish)
UseTags6x HisExpressionInsectMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRIV-Gal4DBD:2xnMagHigh1
Plasmid#182031PurposeDonor plasmid for conversion of KO attP Drosophila lines into ShineGal4 driversDepositorInsertGal4DBD2xHighMag1-p103'UTR
UseDonor plasmidTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rac1 CA
Plasmid#52235PurposeDrosophila expression of YFP-Rac1 constitutively active mutantDepositorInsertRac1 (Rac1 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationQ61L; CA mutationPromoterGAL4Available sinceMarch 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
ORCO-T2A-QF2
Plasmid#162524PurposePlasmid for CRISPR-mediated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-Nb127D01-hIgG
Plasmid#171564PurposeExpresses hIgG-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNb127D01-human IgG-TEV-Avi-His (CXCR2 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT-HGP-v3-NbVHH05-hIgG
Plasmid#171565PurposeExpresses hIgG-tagged anti-UBC6e (human) recombinant alpaca nanobody (NbVHH05-human IgG-TEV-Avi-His) in fly cellsDepositorInsertNbVHH05-human IgG-TEV-Avi-His (UBE2J1 Human)
UseTagsBiP signal peptide and hIgG1-hinge-N297A-TEV site…ExpressionInsectMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-His-VHL-Flag
Plasmid#29497DepositorInsertVon Hippel-Lindau (VHL Human)
UseTagsFlag and HisExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
Plasmid#169369PurposeExpression of gRNA in Culex quinquefasciatus or other mosquitoesDepositorInsertpVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
UseCRISPRTagsExpressionInsectMutationPromoterCPIJ039653Available sinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRB35
Plasmid#72866Purposetemplate base vector for N-terminal tagging, constitutive Puromycin resistanceDepositorInsertbase vector for N-terminal tags, Puro
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(T308A/S473A)
Plasmid#86578PurposeExpresses human full-length Akt1(T308A/S473A) phosphomutantDepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationT308A/S473A phosphomutantPromoterAvailable sinceApril 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAWH-Rluc-let-7-A114-N40-HhR
Plasmid#50555PurposeExpression of luciferase reporter mRNA in S2 cellDepositorInsertLuciferase-let7-A114-N40-HhR
UseLuciferaseTagsExpressionInsectMutationPromoterActin5CAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCoofy51
Plasmid#122005PurposeBaculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage siteDepositorTypeEmpty backboneUseTagsTwinStrepExpressionInsectMutationPromoterAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASp-Egl-Trbo
Plasmid#179993PurposeExpresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.DepositorInsertEgl-Trbo
UseTagsTurboIDExpressionInsectMutationPromoterUAS-Gal4Available sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only