5,013 results
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Plasmid#118892Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal monomeric GFP and HIS6, cleavable with TEVDepositorInsertNcoI-MBP-3C-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsMBP, cleavable with 3C protease and mGFP, HIS6, c…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVL-FLAG/His PALI1
Plasmid#114449PurposeInsect baculovirus expression vector containing N terminal FLAG/His tagged human PALI1 coding sequenceDepositorAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLOT-syb-sp-YFP1-10
Plasmid#73700PurposeNeuronal-synaptobrevin (syb):spYFP1–10 chimera for synaptic GRASPDepositorInsertNeuronal-synaptobrevin:spYFP1–10 chimera
UseLexaop reporter vectorExpressionInsectAvailable SinceMarch 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUAS-kkv-R896K::NG
Plasmid#138957PurposeUsed to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4DepositorArticleInsertkkv-R896K::NG (kkv Fly)
TagsNeon Green fused to C terminus of KkvExpressionInsectMutationR896KPromoterUASAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMT Draper-mCherry
Plasmid#126667PurposeInducible expression of Draper-mCherry in S2 cellsDepositorInsertDraper mCherry
ExpressionInsectMutationC813Y- see depositor comments belowAvailable SinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196E
Plasmid#52975Purposeinsect expression of SIK3 T196E constitutively active mutantDepositorInsertSIK3 (Sik3 Fly)
TagsHAExpressionInsectMutationT196E constitutively active mutantPromoterHsp70Available SinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
ABCB8 (ABCB8A-c016)
Plasmid#110266PurposeBaculovirus expression for structure determination; may not be full ORFDepositorAvailable SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
rNL1(46-638)
Plasmid#20172DepositorInsertNeuroligin 1 (Nlgn1 Rat)
TagsC-terminal 6X His tagExpressionInsectMutationno splice sitesAvailable SinceApril 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUAST-Myc-dATG1
Plasmid#111801PurposeInsect Expression of dAtg1DepositorAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAC8-TwinStrep-3C-DrHDAC6 (40-831)
Plasmid#180226PurposeBaculovirus transfer plasmid to express TwinStrep-3C-DrHDAC6 (40-831)DepositorInsertHistone deacetylase 6 (hdac6 Zebrafish)
TagsTwinStrep-3CExpressionInsectPromoterpolyhedrinAvailable SinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 WT
Plasmid#52972Purposeinsect expression of SIK3 WTDepositorAvailable SinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3GFP
Plasmid#182205Purpose3xTRE Tet-On cassette with 3xp3 GFP marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-GFP
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
DmU6 3p3 GFP LgRNA
Plasmid#182207PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in DrosophilaDepositorInsert3XP3-GFP
UseCRISPRExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC8_PH-GST-Nter-GWs-Lox (VE5586)
Plasmid#163766PurposeTransfer vector for gene expression to generate recombinant baculoviruses by homologous recombination. Contains expression cassette with an N-ter GST tag under the pH promoter.DepositorInsertN-terminal GST tag
TagsGST TagExpressionInsectPromoterPHAvailable SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRB34
Plasmid#72862Purposetemplate for N-terminal 3x FLAG tag, constitutive Blasticidin resistanceDepositorInsert3xFlag tag
ExpressionInsectAvailable SinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pF3BGX-T2A-GAL4
Plasmid#138392PurposeRMCE vector for generating C-terminal T2A-GAL4 fusionDepositorInsertT2A-GAL4
ExpressionInsectAvailable SinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pUAS myc Gr66a
Plasmid#26861DepositorAvailable SinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM394
Plasmid#134211PurposeBaculovirus expression of CENP-N and CENP-LDepositorInsertCENP-N-his-CENP-L
ExpressionInsectAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLib6.4-Cquin_596
Plasmid#176655PurposeExpression of sgRNA under C. quinquefasciatus U6 (CPIJ039596) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organismDepositorTypeEmpty backboneUseCrisprExpressionInsectAvailable SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMos-3xP3DsRed-attP
Plasmid#52904PurposeMos1 transformation vector containing MCS, loxP, attP, 2 x 5HEDepositorTypeEmpty backboneExpressionInsectAvailable SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]
Plasmid#149426PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[Sxl, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbEGFP-NLS
Plasmid#176681PurposeExpression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)DepositorInsertEGFP
ExpressionInsectPromoterAe. aegypti poly-ubiquitin (AAEL003888)Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
OA-1055J
Plasmid#200251PurposepBac-U6-gRNA(Intersex)-3xp3-tdTomatoDepositorInsert2 gRNAs targeting Intersex gene
ExpressionInsectAvailable SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
ArpC4_pLib
Plasmid#173681PurposeArpC4 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expressionDepositorAvailable SinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
AT5G62710 X045_pECIA2
Plasmid#115130PurposeBait vector AT5G62710 X045_pECIA2 should be used with prey vector AT5G62710 X045_pECIA14.DepositorInsertAT5G62710 (AT5G62710 Mustard Weed)
TagsFc, V5 and hexahistidine tagsExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
p10xUAS-IVS- Syn21-Voltron-p10
Plasmid#119041PurposeUAS-driven expresion of Voltron in DrosophilaDepositorInsertVoltron
ExpressionInsectPromoterhsp70Available SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
p2X-UASTattB
Plasmid#189860PurposeFly cloning and transformationDepositorTypeEmpty backboneExpressionInsectAvailable SinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP]
Plasmid#86454PurposeNuclear reporter (histone-tagged EGFP) construct with the Tribolium specific ribosomal protein subunit 3 promoter (driving ubiquitous expression); (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v;Tc'rps3P-Tc'H2Av_EGFP
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only