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Plasmid#72334PurposeTissue-specifically swappable (C-terminal TagRFPT-to-EGP) genome tagging plasmid with Golic+ selection cassette for fully transgenic gene targeting (Drosophila melanogaster)DepositorInsertRRS-TagRFPT-p10-[Golic+] RRS-EGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
Plasmid#127560PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertCas9-T2A-GFP
UseCRISPRTagsExpressionInsectMutationEGFP-D102G see depositor comments belowPromoterAvailable sinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pattB-nsyb-M13::GAL4DBDo
Plasmid#64720Purposegenerating transgenic TRIC fliesDepositorInsertM13::GAL4DBDo
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASt-GBP-Trbo
Plasmid#179996PurposeExpresses two tandem copies of the GFP nanobody fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system. The UASt version is ideal for expression in somatic tissues.DepositorInsertGBP nanobody
UseTagsTrboExpressionInsectMutationPromoterAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSAR
Plasmid#62292Purposedocking site transgenesis plasmid for GoldenGate Cloning and insect transgenesis, DsRed RFP markerDepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceMarch 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBac-GH146-QF
Plasmid#24353DepositorInsertQF
UseTagsdsRedExpressionInsectMutationPromoterAvailable sinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(chimpanzee)
Plasmid#79032PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(chimpanzee)
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOET3-N-TEV-Twin-Strep
Plasmid#203323PurposeGenerate the baculovirus to express C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pQUAST-shibire (ts1)
Plasmid#24362DepositorInsertShibire (ts1) (shi Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 10, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTriEx4-H6-FGB
Plasmid#49203PurposeFluorescent reporter for cGMPDepositorInsertH6-FGB
UseTagsHis tag + Other tagsExpressionBacterial, Insect, and Mamm…MutationNonePromoterCMVAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMT Lifeact-TagBFP
Plasmid#126664PurposeInducible expression of Lifeact-TagBFP in S2 cellsDepositorInsertLifeact-TagBFP fusion
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Nluc-3xP3-RFP
Plasmid#127563PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Nluc-SV40 and 3xP3-RFP visible eye marker.DepositorInsertNluc
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmARC1_N
Plasmid#146978PurposeInsect Expression of DmARC1DepositorInsertDmARC1 (Arc1 Fly)
UseTagsExpressionInsectMutation5 silent mutations and one non silent mutation G3…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
gp64-mVEGF-C-HisTag-pENTR
Plasmid#64065PurposePlasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.DepositorInsertvascular endothelial growth factor C (Vegfc Mouse)
UseTagsHis-tagExpressionInsectMutationPromoterAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati
Plasmid#91800PurposeExpresses the Drosophila dati circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceNov. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFBD-Akt1(WT)
Plasmid#86561PurposeExpresses human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PRExpress-Bxb1-hsp70pA
Plasmid#158391Purposeheat shock inducible Bxb1 integrase for drosophila melanogasterDepositorInsertBxb1
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
T6-pAC5.1 mEGFP-EcoRV
Plasmid#206489PurposeSchneider cell imagingDepositorTypeEmpty backboneUseTagsmEGFPExpressionInsectMutationPromoterAvailable sinceSept. 12, 2023AvailabilityAcademic Institutions and Nonprofits only