4,932 results

1. Ac5-STABLE2-puro

   Plasmid

   #208032

   Purpose

   Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Type

   Empty backbone

   Expression

   Insect

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDC-UAS-nBitbow1.0(miniW)

   Plasmid

   #138329

   Purpose

   Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

   Depositor

   Dawen Cai

   Article

   Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

   Inserts

   mAmetrine
   tdKatushka2
   mNeonGreen
   mTFP1
   mKusabira-Orange2
   

   Tags

   human H2B

   Expression

   Insect

   Promoter

   10xUAS

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac-His-MBP-SCov2-nsp12

   Plasmid

   #154759

   Purpose

   Expression of SARS-CoV-2 nsp12

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp12 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Insect

   Promoter

   Polyhedron

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5

   Plasmid

   #217510

   Purpose

   KD Recombinase dependent conditional smGdP-10XV5 cassette

   Depositor

   S. Lawrence Zipursky

   Article

   Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

   Insert

   KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XV5
   

   Tags

   smGdP-10XV5

   Expression

   Insect

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastbac1-Flag-FANCD2

   Plasmid

   #134904

   Purpose

   codon optimized expression and purification of human FANCD2 in insect cells using baculovirus

   Depositor

   Andrew Deans

   Article

   Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

   Insert

   FANCD2 (FANCD2 Human)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   fully synthetic, codon optimized for insect expre…

   Promoter

   polyhedron

   Available Since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFB1.HMBP.PrS.MTF2

   Plasmid

   #125169

   Purpose

   Expresses human MTF2 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   MTF2 (MTF2 Human)
   

   Tags

   His-MBP

   Expression

   Insect

   Promoter

   Polyhedrin

   Available Since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-IMD-V5-His

   Plasmid

   #63748

   Purpose

   Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter

   Depositor

   Cyril Gueydan

   Insert

   immune deficiency (imd Fly)
   

   Tags

   V5-6XHis

   Expression

   Insect

   Mutation

   *see comment below

   Promoter

   pMT

   Available Since

   April 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. UAS-Syt::7xHalo7

   Plasmid

   #87650

   Purpose

   For creating drosophila transgenics expressing UAS-Syt::7xHalo7

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Syt::7xHalo7
   

   Expression

   Insect

   Mutation

   S265G in Syt (please see depositor's commen…

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Hy_pMT Flag-URH49 WT

   Plasmid

   #110127

   Purpose

   Expresses Flag-tagged human URH49 (DDX39A)

   Depositor

   Jeremy Wilusz

   Article

   Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

   Insert

   URH49 (DDX39A Human)
   

   Tags

   Flag

   Expression

   Insect

   Promoter

   Metallothionein Promoter (pMT)

   Available Since

   April 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHD-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199203

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int
    

    Expression

    Insect

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSTARR-seq_fly

    Plasmid

    #71499

    Purpose

    flySTARR-seq screening vector with DSCP core promoter.

    Depositor

    Alexander Stark

    Article

    Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

    Type

    Empty backbone

    Use

    Flystarr-seq screening vector

    Tags

    sgGFP (SuperGlo, Qbiogene, Inc)

    Expression

    Insect

    Available Since

    Dec. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUASTattb-cAMPr Y205A, R739A

    Plasmid

    #84933

    Purpose

    cAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.

    Depositor

    Justin Blau

    Article

    Hackley et al Sci Signal. 2018 Mar 6;11(520). pii: 11/

    Insert

    cAMPr Y205A R739A
    

    Expression

    Insect

    Mutation

    PKA-C Y205A, PKA-R R739A

    Promoter

    5X UAS

    Available Since

    March 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7-pAc5.1 mCherry EcoRV

    Plasmid

    #206490

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Insect

    Available Since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFBDM

    Plasmid

    #110738

    Purpose

    Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    Polyhedrin and p10 promoters

    Available Since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOCC65

    Plasmid

    #118865

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal monomeric GFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease, mGFP

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAEL010097-Cas9

    Plasmid

    #100707

    Purpose

    The promoter of AAEL010097 drive expression of Streptococcus pyogenes Cas9 gene in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Proc Natl Acad Sci U S A. 2017 Nov 14. p

    Inserts

    AAEL010097-hspcas9-GFP
    Opie2-dsRed
    

    Use

    CRISPR

    Tags

    GFP and dsRED

    Expression

    Insect

    Promoter

    AAEL010097 and Opie2

    Available Since

    Oct. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx-RhoA-wt_mScarlet-i_SGFP2

    Plasmid

    #85071

    Purpose

    Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

    Depositor

    Dorus Gadella

    Article

    Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

    Insert

    RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
    

    Tags

    6xHis and mScarlet-i and SGFP2

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    CMV

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG1a-PAF1C(CTR9/CDC73/PAF1/LEO1/WDR61)

    Plasmid

    #231733

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CTR9 (CTR9 Human)
    CDC73 (CDC73 Human)
    PAF1 (PAF1 Human)
    LEO1 (LEO1 Human)
    WDR61 (SKIC8 Human)
    

    Tags

    3C-Strep(II) and Flag-3C

    Expression

    Insect

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBPGAL4.2Uw-2

    Plasmid

    #26227

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

    Insert

    GAL4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Oct. 7, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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21. mCD8-GFP in pUAST

    Plasmid

    #17746

    Depositor

    Liqun Luo

    Article

    Lee et al Neuron. 1999 Mar . 22(3):451-61.

    Insert

    mouse CD8 (Cd8a Mouse)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    May 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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22. pACE-GIRK2-S

    Plasmid

    #172426

    Purpose

    Expression of full-length, human GIRK2 with C-terminal StrepTag II

    Depositor

    Arthur Laganowsky

    Article

    Qiao et al Biochemistry. 2021 Dec 21;60(50):3813-38

    Insert

    GIRK2 (KCNJ6 Human)
    

    Tags

    Streptag II

    Expression

    Insect

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pBacPAK8-2xHA-MOF

    Plasmid

    #29763

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MYST1 (KAT8 Human)
    

    Tags

    2xHA

    Expression

    Insect

    Available Since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only

24. pFastBac Dual wt p110α/p85α

    Plasmid

    #199302

    Purpose

    co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

    Depositor

    Peter Vogt

    Article

    Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

    Insert

    His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
    

    Tags

    Avi and His

    Expression

    Insect

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. Alpha1

    Plasmid

    #118044

    Purpose

    GoldenBraid2.0 Alpha 1 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBAC-ECFP-15xQUAS_TATA-SV40

    Plasmid

    #104875

    Purpose

    piggyBac vector for cloning 15xQUAS transgenes; used for mosquito tranformation

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    March 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Omega 1

    Plasmid

    #118046

    Purpose

    GoldenBraid2.0 Omega 1 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pUb PspCas13b + PspCas13b guide RNA

    Plasmid

    #176305

    Purpose

    Expresses PspCas13b and its associated guide RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

    Insert

    PspCas13b
    

    Tags

    HA tag

    Expression

    Insect

    Mutation

    E113K, N265S- please depositor comments

    Promoter

    Ubi-p63e

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pMT-Ex

    Plasmid

    #231106

    Purpose

    Inducible protein expression in insect cell culture. Stable cell line generation.

    Depositor

    Michael Jeltsch

    Type

    Empty backbone

    Tags

    6xHis and V5

    Expression

    Insect

    Promoter

    Drosophila metallothionein promoter, MT

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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30. Omega 2

    Plasmid

    #118047

    Purpose

    GoldenBraid2.0 Omega 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Alpha2

    Plasmid

    #118045

    Purpose

    GoldenBraid2.0 Alpha 2 destination vector

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology; Assembly of gb2.0 transcriptio…

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBFv-U6.2

    Plasmid

    #138400

    Purpose

    gRNA expression vector for Drosophila

    Depositor

    Shu Kondo

    Article

    Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

    Type

    Empty backbone

    Expression

    Insect

    Promoter

    U6-2

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pTriEx-NTOM20-mVenus-Zdk2

    Plasmid

    #81011

    Purpose

    Expression of Zdk2 anchored to mitochondria

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    Zdk2
    

    Tags

    6x His tag, FLAG, NTOM20, and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pBIG1e-CDK12deltaRS/Cyclin K

    Plasmid

    #231744

    Purpose

    Protein expression in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Lopez Martinez et al Mol Cell. 2025 May 15;85(10):1952-1967.e

    Inserts

    CDK12 (CDK12 Human)
    CCNK (CCNK Human)
    

    Tags

    Flag-3C

    Expression

    Insect

    Mutation

    CDK12(delta104-380)

    Available Since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZH61 pLIB-8His3C-spt4

    Plasmid

    #231649

    Purpose

    express S.cerevisiae spt4 in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    spt4 (SPT4 Budding Yeast)
    

    Tags

    8His-HRV3C

    Expression

    Insect

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pZH60 pLIB-spt5

    Plasmid

    #231648

    Purpose

    express S.cerevisiae spt5 in insect cells

    Depositor

    Jesper Svejstrup

    Article

    Han et al Mol Cell. 2023 Sep 21;83(18):3253-3267.e

    Insert

    spt5 (SPT5 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA

    Plasmid

    #63166

    Purpose

    GAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-HA"
    

    Tags

    N-myristoylation

    Expression

    Insect

    Available Since

    May 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pSR657

    Plasmid

    #65214

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-2 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    p10 and polyhedrin

    Available Since

    June 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pC-sleeping beauty

    Plasmid

    #65487

    Purpose

    HSP-driven SB100X Sleeping Beauty transposase in pCasper backbone.

    Depositor

    Guillaume Filion

    Insert

    sleeping beauty transposase
    

    Expression

    Insect

    Promoter

    HSP

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBiex-1 AC30_eGFP

    Plasmid

    #82715

    Purpose

    Expresses a GFP-tagged recombinant adenylyl cyclase.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

    Insert

    Adenylyl Cyclase V/II (ADCY2, ADCY5 Human)
    

    Tags

    FLAG purification tag and eGFP

    Expression

    Bacterial and Insect

    Promoter

    T7 Promoter

    Available Since

    Nov. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge