5,038 results
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Plasmid#147340PurposeInsect Expression of DmNot4-dsRNAresDepositorInsertDmNot4-dsRNAres (CG31716 Fly)
ExpressionInsectMutation7 silent mutations and 6 non silent mutations G20…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-Dm4E-T-L36AL39A_T
Plasmid#147604PurposeInsect Expression of Dm4E-T-L36AL39ADepositorInsertDm4E-T-L36AL39A (4E-T Fly)
ExpressionInsectMutationTwo non silent mutations (H628R and N659S) compar…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot3-dsRNAres_Q
Plasmid#147279PurposeInsect Expression of DmNot3-dsRNAresDepositorInsertDmNot3-dsRNAres (Not3 Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4Ga-Y804A-dsRNAres_Q
Plasmid#147274PurposeInsect Expression of DmeIF4Ga-Y80A-dsRNAresDepositorInsertDmeIF4Ga-Y80A-dsRNAres (eIF4G1 Fly)
ExpressionInsectMutationone non silent mutation K340R compared to the seq…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q
Plasmid#147262PurposeInsect Expression of DmNot3-F818A-dsRNAresDepositorInsertDmNot3-F818A-dsRNAres (Not3 Fly)
ExpressionInsectMutationone silent mutation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-10xmut_G
Plasmid#146428PurposeInsect Expression of DmGW182-10xmutDepositorInsertDmGW182-10xmut (gw Fly)
ExpressionInsectMutationMultiple mutations compared to NM_166780.1, see a…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-P1339AP1342A-dsRNAres-V5His6_P
Plasmid#147209PurposeInsect Expression of DmXRN1-P1339AP1342A-dsRNAresDepositorInsertDmXRN1-P1339AP1342A-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmNot1_1710-2505_J
Plasmid#146702PurposeInsect Expression of DmNot1_1710-2505DepositorInsertDmNot1_1710-2505 (Not1 Fly)
ExpressionInsectMutationtwo non silent mutations (A1747T, G1999S) and one…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmCup-Y342AL347A_O
Plasmid#147103PurposeInsect Expression of DmCup-Y342AL347ADepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat-dsRNAres_J
Plasmid#146692PurposeInsect Expression of DmHPat-dsRNAresDepositorInsertDmHPat-dsRNAres (Patr-1 Fly)
ExpressionInsectMutationtwo non silent, A479V, G568D and one silent mutat…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A_R
Plasmid#147394PurposeInsect Expression of CeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389ADepositorInsertCeAIN2_254-400-HsTNRC6C_1207-1690-CIM1-2mut-F1389A (TNRC6C Nematode, Human)
ExpressionInsectMutationDeletion of 2 AA (SD) at position 378-379 compare…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-D207A-dsRNAres-V5His6_O
Plasmid#147065PurposeInsect Expression of DmXRN1-D207A-dsRNAresDepositorInsertDmXRN1-D207A-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1A-DmXRN1-del1323-1355-dsRNAres-V5His6_N
Plasmid#146969PurposeInsect Expression of DmXRN1-del1323-1355-dsRNAresDepositorInsertDmXRN1-del1323-1355-dsRNAres (pcm Fly)
ExpressionInsectMutationone silent mtuation compared to the sequence give…Available SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGW182-delCterm_E
Plasmid#146252PurposeInsect Expression of DmGW182-delC-termDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmSMG6-siRNAres-V5His6_E
Plasmid#146241PurposeInsect Expression of DmSMG6-siRNAresDepositorAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3(K27Q)
Plasmid#182843Purposea K27Q point mutation (AAG/CAG) of Drosophila histone H3 was generated from pAct-GST-H3. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAct-GST-H3
Plasmid#182842PurposeA PCR product encoding GST-H3 (Drosophila histone H3) was was inserted into a modified pAct-5c vector by Sac I and Sal I sites. Expression in S2 cells.DepositorInsertGST-histone H3 fusion
TagsGSTExpressionInsectPromoterActin5CAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔ(N+HAT)
Plasmid#182841PurposeAn EcoRV fragment (encoding residues 1566-2142) was removed from pMT-FLAG-CBPΔN. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP (w Fly)
TagsFLAG tagExpressionInsectMutationdletion of residues 1566-2142 from CBP1476-3222PromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMT-FLAG-CBPΔN
Plasmid#182840PurposeA 5.2 kb Drosophila CBP cDNA fragment (cut by Nhe I and Nsi I), encoding C-terminal residues 1476-3222, was inserted into the pMT-FLAG vector. Expression in S2 cells by CuSO4 induction.DepositorInsertCBP cDNA fragment (cut by Nhe I and Nsi I) (nej Fly)
TagsFLAG tagExpressionInsectPromoterMTAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCasper-pNos-MCP-mCherry
Plasmid#180275Purposeto epress MCP-mCherry fusion protein in the early fly embryos (driven by maternal nanos driver)DepositorInsertMCP-mCherry
TagsmCherryExpressionInsectPromoternanosAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::FRT,attB}
Plasmid#182180Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertmTurquoise
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-EGFP::GW,attB}
Plasmid#182170Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertEGFP
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-mTurquoise::GW,attB}
Plasmid#182169Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertmTurquoise
ExpressionInsectAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xTRE DREaMR 3p3DsR
Plasmid#182206Purpose3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescueDepositorInsertrTetR-3XP3-DsRed
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
1xTRE DREaMR 3p3DsR
Plasmid#182204Purpose1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.DepositorInsertrTetR-3XP3-DsRed
ExpressionInsectAvailable SinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G
Plasmid#146359PurposeInsect Expression of DmDCP2-PBDepositorInsertDmDCP2-PB (DCP2 Fly)
ExpressionInsectMutationContains an in-frame insertion that adds a Q afte…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2_E
Plasmid#146224PurposeInsect Expression of HsAGO2DepositorInsertHsAGO2 (AGO2 Human)
ExpressionInsectMutationContains a K660E mutation compared to the sequenc…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F
Plasmid#146286PurposeInsect Expression of HsAGO2-F2V2DepositorInsertHsAGO2-F2V2 (AGO2 Human)
ExpressionInsectMutationContains F470V, F505V, K660E mutations compared t…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz-E958Q_AH
Plasmid#148884PurposeInsect Expression of DmHelz-E958QDepositorInsertDmHelz-E958Q (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-CR-PA
Plasmid#175445PurposeHiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneTagsBiP Secretion Peptide and Thrombin-Protein AExpressionInsectPromoterFused Actin-HSP70Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
1137B=Hsp70Bb-Cas9
Plasmid#153284PurposeThe attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.DepositorInsertpHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
UseCRISPRTagseGFPExpressionInsectPromoterDmel Hsp70BbAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
1046E=dgRNAs[traB,bTub]
Plasmid#149425PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.DepositorInsertU6.3-gRNAs[TraB, bTub]
UseCRISPRExpressionInsectPromoterU6.3Available SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{ie1-DsRed::FRT,attB}
Plasmid#182190Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-eGFP::GW,attB}
Plasmid#182167Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-dsRed::GW,attB}
Plasmid#182168Purposefluorescent reporter gene with intron containing Gateway cloning cassetteDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-DsRed::FRT-RC,attB}
Plasmid#182187Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-dsRed::KD,attB}
Plasmid#182178Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{3P3-dsRed::FRT,attB}
Plasmid#182176Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-GFP::KD-RC,attB}
Plasmid#182184Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHelz_1213-2103_AH
Plasmid#148883PurposeInsect Expression of DmHelz_1213-2103DepositorInsertDmHelz_1213-2103 (CG9425 Fly)
ExpressionInsectMutationtwo silent an two non silent G1374S, R2076G mutat…Available SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{9Xmef2-eGFP,attB,w+}
Plasmid#182197PurposeEGFP driven by MEF2 transcription factor binding sitesDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{9Xtin-eGFP,w+,attB}
Plasmid#182194PurposeEGFP driven by tinman transcription factor binding sitesDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{3Xtin-GAGA-eGFP,w+,attB}
Plasmid#182195PurposeEGFP driven by tinman and GAGA transcription factor binding sitesDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBac{3Xmef2-eGFP,attB,w+}
Plasmid#182196PurposeEGFP driven by MEF2 transcription factor binding sitesDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{ie1-eGFP::FRT,attB}
Plasmid#182174Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertEGFP
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-DsRed::FRT,attB}
Plasmid#182186Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
p{Mhc-F4-678-DsRed::KD-RC,attB}
Plasmid#182189Purposefluorescent reporter gene with yeast recombination site within intronDepositorInsertDsRed
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y
Plasmid#148080PurposeInsect Expression of DmPAN3-dsRNAresDepositorInsertDmPAN3-dsRNAres (CG11486 Fly)
ExpressionInsectAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only