Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

5,017 results

Showing: 4201 - 4250 of 5017 results

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p{3P3-mTurquoise::GW,attB}

Plasmid

#182169

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Expression

Insect

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
3xTRE DREaMR 3p3DsR

Plasmid

#182206

Purpose

3xTRE Tet-On cassette with 3xp3 DsRed maker for generating knockins with doxycycline-inducible rescue

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Expression

Insect

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1xTRE DREaMR 3p3DsR

Plasmid

#182204

Purpose

1xTRE Tet-On cassette with 3xp3 DsRed marker for generating knockins with doxycycline-inducible rescue.

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

rTetR-3XP3-DsRed

Expression

Insect

Available Since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP2-PB-V5His6_G

Plasmid

#146359

Purpose

Insect Expression of DmDCP2-PB

Depositor

Elisa Izaurralde

Insert

DmDCP2-PB (DCP2 Fly)

Expression

Insect

Mutation

Contains an in-frame insertion that adds a Q afte…

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-HsAGO2_E

Plasmid

#146224

Purpose

Insect Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2 (AGO2 Human)

Expression

Insect

Mutation

Contains a K660E mutation compared to the sequenc…

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-HsAGO2-F2V2_F

Plasmid

#146286

Purpose

Insect Expression of HsAGO2-F2V2

Depositor

Elisa Izaurralde

Article

Eulalio et al Nat Struct Mol Biol. 2008 Apr . 15(4):34

Insert

HsAGO2-F2V2 (AGO2 Human)

Expression

Insect

Mutation

Contains F470V, F505V, K660E mutations compared t…

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmHelz-E958Q_AH

Plasmid

#148884

Purpose

Insect Expression of DmHelz-E958Q

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz-E958Q (CG9425 Fly)

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Available Since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pExpreS2-1-CR-PA

Plasmid

#175445

Purpose

HiFi-compatible destination vector for secreted overexpression of thrombin-cleavable, C-terminal Protein A tagged proteins with ExpreS2 Platform

Depositor

Wyatt Yue

Article

Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

Type

Empty backbone

Tags

BiP Secretion Peptide and Thrombin-Protein A

Expression

Insect

Promoter

Fused Actin-HSP70

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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BicC.Ca9.R

Plasmid

#168295

Purpose

Expresses Cas9 in Drosophila late germline cells

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

BicC.Cas9-T2A-eGFP-p10

Use

CRISPR

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1137B=Hsp70Bb-Cas9

Plasmid

#153284

Purpose

The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)

Use

CRISPR

Tags

eGFP

Expression

Insect

Promoter

Dmel Hsp70Bb

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1046E=dgRNAs[traB,bTub]

Plasmid

#149425

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Insert

U6.3-gRNAs[TraB, bTub]

Use

CRISPR

Expression

Insect

Promoter

U6.3

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

Plasmid

#149427

Purpose

The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

Depositor

Omar Akbari

Article

Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

Inserts

U6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10

Use

CRISPR

Tags

eGFP

Expression

Insect

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{ie1-DsRed::FRT,attB}

Plasmid

#182190

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-eGFP::GW,attB}

Plasmid

#182167

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::GW,attB}

Plasmid

#182168

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-DsRed::FRT-RC,attB}

Plasmid

#182187

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::KD,attB}

Plasmid

#182178

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::FRT,attB}

Plasmid

#182176

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-GFP::KD-RC,attB}

Plasmid

#182184

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmHelz_1213-2103_AH

Plasmid

#148883

Purpose

Insect Expression of DmHelz_1213-2103

Depositor

Elisa Izaurralde

Article

Hanet et al Life Sci Alliance. 2019 Sep 30;2(5). pii

Insert

DmHelz_1213-2103 (CG9425 Fly)

Expression

Insect

Mutation

two silent an two non silent G1374S, R2076G mutat…

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{9Xmef2-eGFP,attB,w+}

Plasmid

#182197

Purpose

EGFP driven by MEF2 transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{9Xtin-eGFP,w+,attB}

Plasmid

#182194

Purpose

EGFP driven by tinman transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{3Xtin-GAGA-eGFP,w+,attB}

Plasmid

#182195

Purpose

EGFP driven by tinman and GAGA transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{3Xmef2-eGFP,attB,w+}

Plasmid

#182196

Purpose

EGFP driven by MEF2 transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{ie1-eGFP::FRT,attB}

Plasmid

#182174

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-DsRed::FRT,attB}

Plasmid

#182186

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-DsRed::KD-RC,attB}

Plasmid

#182189

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3-dsRNAres_Y

Plasmid

#148080

Purpose

Insect Expression of DmPAN3-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN3-dsRNAres (CG11486 Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN2-F1103A-dsRNAres_Y

Plasmid

#148075

Purpose

Insect Expression of DmPAN2-F1103A-dsRNAres

Depositor

Elisa Izaurralde

Article

Jonas et al Nat Struct Mol Biol. 2014 Jul;21(7):599-

Insert

DmPAN2-F1103A-dsRNAres (PAN2 Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-dsRed::KD-RC,attB}

Plasmid

#182179

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBac{3Xtin-eGFP,w+,attB}

Plasmid

#182193

Purpose

EGFP driven by tinman transcription factor binding sites

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-eGFP::KD,attB}

Plasmid

#182183

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCaf40-F65AC69Y_X

Plasmid

#147993

Purpose

Insect Expression of DmCaf40-F65AC69Y

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40-F65AC69Y (Rcd-1 Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-GST-DmNanos_116-163-E151AF152AN155A-V5His6_AC

Plasmid

#148466

Purpose

Insect Expression of DmNanos_116-163-E151AF152AN1555A

Depositor

Elisa Izaurralde

Article

Raisch et al EMBO J. 2016 May 2;35(9):974-90. doi: 10

Insert

DmNanos_116-163-E151AF152AN1555A (nos Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmGW182_1200-1384_L

Plasmid

#146825

Purpose

Insect Expression of DmGW182_1200-1384

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

DmGW182_1200-1384 (gw Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{Mhc-F4-678-DsRed::KD,attB}

Plasmid

#182188

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
p{3P3-mTurquoise::KD,attB}

Plasmid

#182182

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

mTurquoise

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmRUMP-del233-341_X

Plasmid

#147920

Purpose

Insect Expression of DmRUMP-del233-341

Depositor

Elisa Izaurralde

Insert

DmRUMP-del233-341 (rump Fly)

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmCaf40_25-291_W

Plasmid

#147866

Purpose

Insect Expression of DmCaf40_25-291

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmCaf40_25-291 (Rcd-1 Fly)

Expression

Insect

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147860

Purpose

Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1_812-1945-dsRNAres_W

Plasmid

#147845

Purpose

Insect Expression of DmNot1_812-1945-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_812-1945-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

3 silent mutations compared to the sequence given…

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMT-EGFP-CTAP-PrP-SBP_W

Plasmid

#147835

Purpose

Insect Expression of EGFP

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

EGFP

Expression

Insect

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot2-del458-482-dsRNAres_U

Plasmid

#147716

Purpose

Insect Expression of DmNot2-del458-482-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del458-482-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot2-del393-429-W556E-dsRNAres_U

Plasmid

#147698

Purpose

Insect Expression of DmNot2-del393-429-W556E-dsRNAres

Depositor

Elisa Izaurralde

Article

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

DmNot2-del393-429-W556E-dsRNAres (Rga Fly)

Expression

Insect

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmPAN3-D575RL676EG683E_U

Plasmid

#147670

Purpose

Insect Expression of DmPAN3-D575RL676EG683E

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3-D575RL676EG683E (CG11486 Fly)

Expression

Insect

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmPAN3-L676EG683ES708AT710A_U

Plasmid

#147662

Purpose

Insect Expression of DmPAN3-L676EG683ES708AT710A

Depositor

Elisa Izaurralde

Article

Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

Insert

DmPAN3-L676EG683ES708AT710A (CG11486 Fly)

Expression

Insect

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-HA-DmPop2a-dsRNAres-V5His6_K

Plasmid

#146717

Purpose

Insect Expression of DmPop2a-dsRNAres

Depositor

Elisa Izaurralde

Insert

DmPop2a-dsRNAres (Pop2 Fly)

Expression

Insect

Mutation

ORF extracted from CG5684-Pa; NM_140281.2, with o…

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1_1674-1962-dsRNAres_AC

Plasmid

#148402

Purpose

Insect Expression of DmNot1_1674-1962-dsRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmNot1_1674-1962-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmYTHDFa_546-700_AC

Plasmid

#148384

Purpose

Insect Expression of DmYTHDFa_546-700

Depositor

Elisa Izaurralde

Insert

DmYTHDFa_546-700 (CG6422 Fly)

Expression

Insect

Mutation

1 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmMextli-M605D_AB

Plasmid

#148344

Purpose

Insect Expression of DmMextli-M605D

Depositor

Elisa Izaurralde

Article

Peter et al Genes Dev. 2015 Sep 1;29(17):1835-49. do

Insert

DmMextli-M605D (mxt Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge

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