74,970 results

1. pGP-CMV-jGCaMP8m

   Plasmid

   #162372

   Purpose

   Mammalian expression of ultrafast protein calcium sensor

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Insert

   jGCaMP8m
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   A25G F286Y

   Promoter

   CMV

   Available since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS

   Plasmid

   #187588

   Purpose

   Express FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cells

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Insert

   NLS
   

   Use

   Tags

   APEX2-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. RFP Dynamin2 Wild Type

   Plasmid

   #128152

   Purpose

   Expression of Dynamin 2

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Chua et al Proc Natl Acad Sci U S A. 2009 Nov 30. (

   Insert

   Dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-Mito4x-jRCaMP1b

   Plasmid

   #127873

   Purpose

   Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Red-shifted mitochondrial matrix Ca2+ indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDEST-cDNA5-FRT/TO-3*Flag-APEX2 N-term

   Plasmid

   #182925

   Purpose

   Express FLAG epitope and APEX2-tagged fusion protein in mammalian cells.

   Depositor

   Benjamin Blencowe

   Article

   Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

   Type

   Empty backbone

   Use

   Tags

   APEX2 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. tdTomato-PDHA1-N-10

   Plasmid

   #58124

   Purpose

   Localization: Mitochondria, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   PDHA1 (PDHA1 Human)
   

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. LBR EGFP

   Plasmid

   #128150

   Purpose

   Expression of lamin B receptor

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Ellenberg et al J Cell Biol. 1997 Sep 22. 138(6):1193-20

   Insert

   lamin B receptor (LBR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nsp12-EGFP

   Plasmid

   #165116

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp12 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-CXCR4

   Plasmid

   #98942

   Purpose

   Mammalian expression plasmid for untagged human CXCR4

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo Fluc EGFP

    Plasmid

    #90170

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBRPB CAG-mCherry-IP

    Plasmid

    #106333

    Purpose

    PiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.

    Depositor

    Thomas Tuschl

    Article

    Yamaji et al Nature. 2017 Mar 23;543(7646):568-572. d

    Insert

    mCherry-IRES-Puro
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid

    #55202

    Purpose

    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    dsRed2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. Bxb1-GT_AIO-TetOn_Divergent Donor

    Plasmid

    #229784

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Bxb1-GA_AIO-TetOn_Divergent Donor

    Plasmid

    #229786

    Purpose

    Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    LacZ; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAG-luciferase

    Plasmid

    #55764

    Purpose

    luciferase reporter for in vivo bioluminescence imaging

    Depositor

    Snorri Thorgeirsson

    Article

    Lee et al Cancer Res. 2010 Nov 1;70(21):8264-9. do

    Insert

    CMV enhancer/chicken beta actin promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV enhancer/chicken beta actin promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. CaV2.1/pcDNA3.1 ~ Human

    Plasmid

    #140575

    Purpose

    Expression in mammallian cells or oocytes

    Depositor

    Dan Minor

    Article

    Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

    Insert

    CaV2.1 Human (CACNA1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX302 Luciferase-V5 puro

    Plasmid

    #47553

    Purpose

    Lentiviral expression of V5-tagged Luciferase in mammalian cells

    Depositor

    Kevin Janes

    Article

    Kang et al Sci Signal. 2013 Aug 6;6(287):rs13. doi:

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    None

    Promoter

    CMV

    Available since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-TSC2

    Plasmid

    #15478

    Depositor

    Do-Hyung Kim

    Article

    Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

    Insert

    TSC2 shRNA (TSC2 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA5-FRT/TO-3*Flag-APEX2 C-term SRSF7

    Plasmid

    #187582

    Purpose

    Express FLAG epitope and APEX2-tagged SRSF7 fusion protein in mammalian cells

    Depositor

    Benjamin Blencowe

    Article

    Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

    Insert

    SRSF7 (SRSF7 Human)
    

    Use

    Tags

    APEX2-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. myc-BioID2-MCS

    Plasmid

    #74223

    Purpose

    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present downstream of myc tag and BioID2 tag

    Depositor

    Kyle Roux

    Article

    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Tags

    Myc and humanized A. aeolicus BioID2 with R40G

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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