74,970 results

1. pCW57.1-MAT2A

   Plasmid

   #100521

   Purpose

   Dox-repressible cDNA expression of MAT2A (dox-off/ tet-off all-in-one vector)

   Depositor

   David Sabatini

   Article

   Gu et al Science. 2017 Nov 10;358(6364):813-818.

   Insert

   MAT2A (MAT2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available since

   Jan. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-CXCR4

   Plasmid

   #98942

   Purpose

   Mammalian expression plasmid for untagged human CXCR4

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CXCR4 (CXCR4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-Mito4x-jRCaMP1b

   Plasmid

   #127873

   Purpose

   Red-shifted fluorescent reporter for mitochondrial matrix Ca2+

   Depositor

   Timothy Ryan

   Article

   Ashrafi et al Neuron. 2019 Dec 10. pii: S0896-6273(19)

   Insert

   Red-shifted mitochondrial matrix Ca2+ indicator
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ (M)-HT-Cbx2

   Plasmid

   #82510

   Purpose

   Expresses Halotag-Cbx2 fusion proteins in mammalian cells

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 2 (CBX2 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWPXL

   Plasmid

   #12257

   Purpose

   2nd generation lentiviral transfer plasmid

   Depositor

   Didier Trono

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2+IMS-HyPer7

   Plasmid

   #136469

   Purpose

   Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

   Depositor

   Vsevolod Belousov

   Article

   Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

   Insert

   HyPer7
   

   Use

   Tags

   SMAC/DIABLO

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, SP6

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-hAR

   Plasmid

   #89078

   Purpose

   mammalian expression of human androgen receptor in pCMV5

   Depositor

   Elizabeth Wilson

   Article

   Lubahn et al Science. 1988 Apr 15;240(4850):327-30.

   Insert

   human androgen receptor (hAR) full-length (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

   Plasmid

   #55202

   Purpose

   Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.

   Depositor

   Timothy Lu

   Article

   Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

   Insert

   dsRed2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFX-EGFP-EEA1

   Plasmid

   #174451

   Purpose

   Express EGFP with early endosome-targeting sequence in mammalian cells

   Depositor

   Yusuke Ohba

   Article

   Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

   Insert

   EGFP with early endosome-targeting sequence
   

   Use

   Tags

   early endosomal antigen 1 (EEA1)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCI-neo Fluc EGFP

    Plasmid

    #90170

    Purpose

    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins

    Depositor

    Franz-Ulrich Hartl

    Article

    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

    Insert

    FLuc EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-IRES-mAmetrine 1.1 FP

    Plasmid

    #52113

    Purpose

    a bicistronic IRES-mAtetrine containing retroviral vector

    Depositor

    Dario Vignali

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-mAmetrine 1.1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-HA-NONO

    Plasmid

    #127655

    Purpose

    Over-express HA-tagged human NONO

    Depositor

    Nicolas Manel

    Article

    Lahaye et al Cell. 2018 Oct 4;175(2):488-501.e22. doi

    Insert

    NONO (NONO Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ8001 - pCMV-R8.91 (D64V)

    Plasmid

    #202687

    Purpose

    R8.91 plasmid with mutated integrase (D64V)

    Depositor

    Stanley Qi

    Article

    Chavez et al Nat Biomed Eng. 2023 May 1. doi: 10.1038

    Insert

    gag pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D64V

    Promoter

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. Dual-sgRNA_hU6-mU6

    Plasmid

    #154194

    Purpose

    Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

    Depositor

    Anna Obenauf

    Article

    Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. TFORF1818

    Plasmid

    #142860

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    UHRF1 (UHRF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. All-in-one lenti-ABE8e-NG

    Plasmid

    #200447

    Purpose

    Lentivirus vector for base editing

    Depositor

    Benjamin Cravatt

    Article

    Li et al Nat Chem Biol. 2023 Nov;19(11):1320-1330

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. CLYBL_Bxb1-GA_LP_Dual_TC

    Plasmid

    #229776

    Purpose

    CLYBL targeting construct with the Bxb1-GA landing pad cassette.

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    FRT-mScarlet-attP(GA)-PuroR-F3
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pGK

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti sgRNA(MS2)_puro optimized backbone

    Plasmid

    #73797

    Purpose

    optimized lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-PE2max-BSD

    Plasmid

    #191102

    Purpose

    Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    PEmax-P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9

    Promoter

    EF-1a

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV CEPIA2mt

    Plasmid

    #58218

    Purpose

    Green fluorescent indicator for calcium imaging in the mitochondria

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    CEPIA2mt
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    GCaMP2 N263Y E282V M339L

    Promoter

    CMV

    Available since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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