75,142 results

1. pGP-pcDNA3.1 Puro-CAG-Voltron2-ST

   Plasmid

   #172910

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Voltron2-ST
   

   Use

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-tdTomato (codon diversified)

   Plasmid

   #59462

   Purpose

   AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter

   Depositor

   Edward Boyden

   Has Service

   AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   codon diversified

   Promoter

   CAG

   Available since

   Sept. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-CreERT2

   Plasmid

   #14797

   Purpose

   Mammalian expression of conditionally active form of Cre recombinase (not as tightly regulated as pCAG-ERT2CreERT2)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   Cre-ERT2 fusion protein
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cas9-GFP_sg_mTfeb

   Plasmid

   #79006

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   sgTfeb (Tfeb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TFORF2925

   Plasmid

   #143676

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZFP57 (ZFP57 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHCMV-VSVgSS-pAG-TM

   Plasmid

   #207318

   Purpose

   Functional incorporation of protein AG (pAG) fusion protein into envelope of virions

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   protein AG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. FAT FAK biosensor

   Plasmid

   #78303

   Purpose

   FRET biosensor. Visualization of FAK activity at focal adhesions in live cells

   Depositor

   Yingxiao Wang

   Article

   Seong et al Proc Natl Acad Sci U S A. 2013 Nov 26;11

   Insert

   FAT FAK biosensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

8. MIP 227 CoMiP 4in1 wo shRNA p53

   Plasmid

   #63727

   Purpose

   To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

   Depositor

   Mark Kay, Joseph Wu

   Article

   Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

   Insert

   Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC Human)
   

   Use

   Tags

   IRES-dtomato

   Expression

   Mammalian

   Mutation

   codon-optimised

   Promoter

   SFFV

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-EFS-SpCas9

   Plasmid

   #104588

   Purpose

   AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   Streptococcus pyogenes Cas9
   

   Use

   AAV and CRISPR

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7

    Plasmid

    #175526

    Purpose

    Co-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Lifeact-HaloTag11 and Tomm20-HaloTag7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p246 eSpCas9_2gRNAs_mH11

    Plasmid

    #164854

    Purpose

    gRNA vector for targeting mouse H11 locus

    Depositor

    Philip Jordan

    Article

    Pryzhkova et al Stem Cell Res. 2020 Nov 5;49:102078. doi

    Insert

    gRNAs for targeting mouse H11 locus
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEFIRES-P-EGFP-ADRP

    Plasmid

    #87157

    Purpose

    Fluorescent protein localized to lipid droplets (LDs)

    Depositor

    Elina Ikonen

    Article

    Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

    Insert

    ADRP (PLIN2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    mCherry-R TTGGTCACCTTCAGCTTGG

    Available since

    May 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-TRIF-CFP

    Plasmid

    #13644

    Depositor

    Doug Golenbock

    Insert

    TRIF (TICAM1 Human)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-EF1-FHC

    Plasmid

    #64874

    Purpose

    lentiviral vector with Flag, HA tags

    Depositor

    Richard Wood

    Article

    Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCG-Hc∆18

    Plasmid

    #84817

    Purpose

    Measles virus hemagglutinin expressing plasmid

    Depositor

    Jakob Reiser

    Article

    Marino et al Gene Ther. 2015 Mar;22(3):280-5. doi: 10

    Insert

    Measles virus H glycoprotein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST53-LRRK2-WT

    Plasmid

    #25044

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENN.AAV.CMVs.Pl.Cre.rBG

    Plasmid

    #105537

    Purpose

    AAV expression of Cre from CMV promoter

    Depositor

    James M. Wilson

    Has Service

    AAV rh10, AAV1, AAV2, AAV5, AAV8, and AAV9

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHM6-alphasynuclein-A53T

    Plasmid

    #40825

    Depositor

    David Rubinsztein

    Article

    Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    6x His and HA

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    CMV

    Available since

    May 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. T7-VEE-OKS-iG

    Plasmid

    #58974

    Purpose

    RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, KLF4, SOX2 separated by 2A peptides followed by an IRES then GLIS1 and a second IRES and PuroR ORF

    Depositor

    Steven Dowdy

    Article

    Yoshioka et al Cell Stem Cell. 2013 Aug 1;13(2):246-54.

    Inserts

    Oct4 (POU5F1 Human)
    Klf4 (KLF4 Human)
    Sox2 (SOX2 Human)
    GLIS (GLIS1 Human)
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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