81,094 results

Showing: 4201 - 4230 of 81094 results

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pY30

Plasmid

#84745

Purpose

Expresses huAsCpf1-P2A-puro and crRNA guide

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Inserts

huAsCpf1
puromycin resistance gene

Tags

3xHA, NLS, and T2A

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Article

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available Since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

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CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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AAVS1-Blasticidin-CAG-Flpe-ERT2

Plasmid

#68461

Purpose

AAVS1 targeting donor plasmid with Flpe-ERT2 expression cassette and blasticidin selection gene

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

mammalian-codon-optimized Flpe recombinase fused with ERT2

Use

Synthetic Biology

Tags

ERT2

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pmCTIP2-N106

Plasmid

#66808

Purpose

2nd generation lentiviral transfer plasmid. Expresses mouse CTIP2 with blasticidin resistance

Depositor

Andrew Yoo

Article

Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

Insert

CTIP2 isoform b (Bcl11b Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

Human EF1a

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEYFP-Sec24C

Plasmid

#66608

Purpose

YFP fusion of Sec24C

Depositor

David Stephens

Article

Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

Insert

Sec24C (SEC24C Human)

Expression

Mammalian

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-BRD7

Plasmid

#65379

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD7 (BRD7 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-TRIM28

Plasmid

#65397

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

TRIM28 (TRIM28 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-dCas9-FLp300

Plasmid

#61356

Purpose

encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

Depositor

Charles Gersbach

Article

Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

Insert

S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 S. pyogenes, Synthetic, Human)

Use

CRISPR and Synthetic Biology

Tags

Flag, HA, and SV40 NLS

Expression

Mammalian

Mutation

D10A; H840A in S.pyogenes Cas9

Promoter

CMV

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only

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pMarsL274G

Plasmid

#63177

Purpose

This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

Depositor

David Tirrell

Article

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Insert

MarsL274G (Mars1 Mouse)

Expression

Mammalian

Mutation

L274G

Promoter

CMV

Available Since

April 10, 2015

Availability

Academic Institutions and Nonprofits only

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YFP NLS Beta-Actin

Plasmid

#60613

Purpose

Encodes YFP and NLS tagged beta-actin

Depositor

Primal de Lanerolle

Article

Chang et al Proc Natl Acad Sci U S A. 2011 May 24;10

Insert

Beta-actin (ACTB Human)

Tags

EYFP and NLS

Expression

Mammalian

Available Since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT/TO SF-HERC2 (ShB-R)

Plasmid

#55613

Purpose

Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cells

Depositor

David Chan

Article

Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

Insert

3xFLAG tagged HERC2 (WT) (HERC2 Human)

Tags

3xFLAG and STREP

Expression

Mammalian

Mutation

Silent mutations have been incorporated into the …

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only

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p2attPC

Plasmid

#51547

Purpose

DICE donor plasmid without neural transcription factors.

Depositor

Michele Calos

Article

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

mCherry

Expression

Mammalian

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pEYFP-Mitotrap

Plasmid

#46942

Purpose

Expresses Mitotrap in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Mitotrap (MTOR Aequorea victoria, Budding Yeast, Synthetic, Human)

Tags

This is a fusion between a yeast mitochondrial ta…

Expression

Mammalian

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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HA Sec13 pRK5

Plasmid

#46332

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Sec13 (SEC13 Human)

Tags

HA

Expression

Mammalian

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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FLAG-SNAP29

Plasmid

#45915

Depositor

Noboru Mizushima

Article

Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

Insert

synaptosomal-associated protein, 29kDa (SNAP29 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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MSCV-N E4orf1

Plasmid

#38063

Depositor

Karl Munger

Article

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

E4 orf1

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available Since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

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pCLucf

Plasmid

#37328

Depositor

John Schiller

Article

Johnson et al J Virol. 2009 Mar;83(5):2067-74. Epub 20

Inserts

Luciferase (luciferase Fly)
GFP

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pECE-M2-BAIAP2 wt

Plasmid

#31656

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

BAI1-associated protein 2 (BAIAP2 Human)

Tags

M2

Expression

Mammalian

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pCMX-Gal4-TEAD2

Plasmid

#33107

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD2 (TEAD2 Human)

Tags

Gal4

Expression

Mammalian

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

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GFP- RPL26

Plasmid

#31980

Depositor

Michael Kastan

Article

Takagi et al Cell. 2005 Oct 7. 123(1):49-63.

Insert

RPL26 (RPL26 Human)

Tags

GFP

Expression

Mammalian

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pSG5-ABL

Plasmid

#31284

Depositor

Nora Heisterkamp

Article

Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

Insert

V-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1 Human)

Expression

Mammalian

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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MSCV puro let-7 sponge

Plasmid

#29766

Depositor

Phil Sharp

Article

Kumar et al Proc Natl Acad Sci U S A. 2008 Mar 11;10

Insert

let-7 sponge

Use

Retroviral

Expression

Mammalian

Available Since

June 24, 2011

Availability

Academic Institutions and Nonprofits only
C32V

Plasmid

#26396

Depositor

Steven Vogel

Article

Koushik et al Biophys J. 2006 Dec 15. 91(12):L99-L101.

Insert

Cerulean-32-Venus

Use

Fret standard

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA3

Plasmid

#26679

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 3 (KPNA3 Human)

Tags

T7 (MASMTGGQQMG)

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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p3XFLAG-MKL2

Plasmid

#27175

Purpose

For mammalian expression of human MKL2 with a 3x FLAG tag

Depositor

Ron Prywes

Article

Selvaraj et al J Biol Chem. 2003 Oct 24. 278(43):41977-

Insert

MKL2 (MRTFB Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

Dec. 22, 2010

Availability

Academic Institutions and Nonprofits only
pF150 pSOD1G93AAcGFP1

Plasmid

#26411

Purpose

contains G94A mutation in pSOD1

Depositor

Elizabeth Fisher

Article

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Tags

GFP

Expression

Mammalian

Mutation

G94A

Available Since

Nov. 8, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-Myc S6K1

Plasmid

#26610

Depositor

Jie Chen

Article

Fang et al Science. 2001 Nov 30. 294(5548):1942-5.

Insert

S6K1 (Rps6kb1 Rat)

Tags

Myc

Expression

Mammalian

Available Since

Oct. 29, 2010

Availability

Academic Institutions and Nonprofits only
Flag-HA-USP53

Plasmid

#22606

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP53 (USP53 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

not FL, missing first 65 aa

Promoter

CMV/TO

Available Since

Nov. 17, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-HA-YOD1

Plasmid

#22554

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

YOD1 (YOD1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge

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81,094 results