78,024 results

1. AAV-hGFAP-mCherry

   Plasmid

   #178581

   Purpose

   Expression of mCherry under the human GFAP promoter

   Depositor

   Chun-Li Zhang

   Article

   Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

   Insert

   mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. eSpCas9(1.1)_NoFlag_hROSA26_Left

   Plasmid

   #191442

   Purpose

   Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus

   Depositor

   Yannick Doyon

   Insert

   hROSA26 sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-MMLVgag-3xNES-ABE8e

   Plasmid

   #181751

   Purpose

   MMLV-Gag fused to 3xNES-ABE8e with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.

   Depositor

   David Liu

   Article

   Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014

   Insert

   MMLVgag-3xNES-ABE8e
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT306 Kp03 Lenti pEF-attB LSR GFP

   Plasmid

   #184949

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Lacra Bintu, Michael Bassik, Patrick Hsu, Ami Bhatt

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Kp03 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-mt-mKeima-P2A-FRB-Fis1

   Plasmid

   #135295

   Purpose

   stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

   Depositor

   Richard Youle

   Article

   Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

   Insert

   mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

   Available Since

   Jan. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA

   Plasmid

   #210501

   Purpose

   expresses LAUNCHER component in mammalian cells

   Depositor

   Dongmin Lee

   Article

   Cui et al Nat Chem Biol. 2024 Mar;20(3):353-364. d

   Insert

   PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-tTA
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0024

   Plasmid

   #142528

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXQ1 (FOXQ1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-GLIS1

   Plasmid

   #30166

   Purpose

   Retroviral expression of non-canonical human GLIS1

   Depositor

   Shinya Yamanaka

   Article

   Maekawa et al Nature. 2011 . 474(7350):225-9.

   Insert

   GLIS1 (GLIS1 Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   June 10, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBI-p53 R175H/EGFP

   Plasmid

   #16544

   Depositor

   Bert Vogelstein

   Article

   Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

   Insert

   p53 (TP53 Human)
   

   Expression

   Mammalian

   Mutation

   R175H

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. DsRed-rab11 DN

    Plasmid

    #12680

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    changed serine 25 to asparagine

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG RpH-LAMP1-3xFLAG

    Plasmid

    #163018

    Purpose

    expresses ratiometric sensor of lysosomal lumenal pH

    Depositor

    Massimiliano Stagi

    Article

    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

    Insert

    RpH-LAMP1-3xFLAG (Lamp1 Mouse)
    

    Tags

    3XFLAG

    Expression

    Mammalian

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. PEmax_2star_IVT

    Plasmid

    #227674

    Purpose

    plasmid for PEmax** mRNA IVT

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

    Insert

    PEmax_2_star
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-hSNCA-NE

    Plasmid

    #102361

    Purpose

    Overexpression of synuclein alpha in mammalian cells

    Depositor

    Shu Leong Ho

    Article

    Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

    Insert

    synuclein alpha (SNCA Human)
    

    Tags

    NE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-ApoE3

    Plasmid

    #197147

    Purpose

    Expresses ApoE3 in Mammalian cell

    Depositor

    Bradley Hyman

    Article

    Hudry et al Sci Transl Med. 2013 Nov 20;5(212):212ra

    Insert

    ApoE3 (APOE Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3 Flag beta-2-adrenergic-receptor

    Plasmid

    #14697

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B2AR (ADRB2 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. p4xmts-mScarlet3_N1

    Plasmid

    #189774

    Purpose

    Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    4xmts-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pQ79

    Plasmid

    #175064

    Purpose

    expresses mutant of the huntingtin exon1 in mammalian cells

    Depositor

    Arnold Boersma

    Article

    Wan et al Cell Rep Methods. 2022 Mar 28;2(3):10018

    Insert

    polyQ79 (HTT Human)
    

    Expression

    Mammalian

    Mutation

    mVenus: C-Terminal deletion of 11 aa (GITLGMDELYK…

    Promoter

    CMV Promoter

    Available Since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMF1706

    Plasmid

    #125582

    Purpose

    Expresses peroxisomal roGFP2 (po-roGFP2) in mammalian cells

    Depositor

    Marc Fransen

    Article

    Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

    Insert

    po-roGFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEGFP-LC3

    Plasmid

    #21073

    Purpose

    Mammalian expression of rat LC3 fused to EGFP

    Depositor

    Tamotsu Yoshimori

    Article

    Kabeya et al EMBO J. 2000 Nov 1. 19(21):5720-8.

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    First ATG is not included.

    Promoter

    CMV

    Available Since

    May 11, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-eGFP-uTEV3

    Plasmid

    #234320

    Purpose

    Mammalian expression of uTEV3 tagged with an eGFP marker

    Depositor

    Mateo Sánchez

    Article

    Silva-Rojas et al Nat Biomed Eng. 2025 Jun 5. doi: 10.1038

    Insert

    eGFP-uTEV3
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Mutations respective to the wild type TEV: S219V …

    Promoter

    CMV

    Available Since

    June 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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