82,515 results
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Plasmid#105539PurposeAAV expression of EGFP from hSyn promoterDepositorHas serviceAAV1InsertEGFP
UseAAVExpressionMammalianPromoterhSynAvailable sinceMarch 1, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
JG1211: CAG-human dLbCpf1(D832A)-NLS-3xHA-VPR
Plasmid#104567PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to VPR activatorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman codon optimized ‘dead’ Cpf1 fused to HSV VPR activation domain
UseCRISPRTagsNLS-3xHA-VPRExpressionMammalianMutationD832APromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.Syn.GCaMP6s.WPRE.SV40
Plasmid#100843PurposeAAV expression of ultrasensitive protein calcium sensor from Synapsin promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6s
UseAAVExpressionMammalianMutationGCaMP3-K78H T302L R303P D380Y T381R S383T R392GPromoterSynapsinAvailable sinceNov. 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-pro-Il1b-C-Flag
Plasmid#75131PurposeExpresses C-terminal flag-tagged pro-IL-1β in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertinterleukin 1 beta (Il1b Mouse)
Tags3XFLAGExpressionMammalianMutation(please see depositor comments below)PromoterCMVAvailable sinceSept. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLuc EGFP
ExpressionMammalianMutationR188Q, R261Q in FLucAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIP-AMPKa1_WT
Plasmid#79010PurposeFor mammalian expression of WT AMPK alpha1.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAMPK alpha 1 (PRKAA1 Human)
ExpressionMammalianPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-Flag::UbvG08 I44A, deltaGG
Plasmid#74939PurposeUbiquitin variant that binds to and occludes the ligand binding site of the 53BP1 Tudor domain (i53). Blocks 53BP1 from accumulating at sites of DNA damage. Potent selective inhibitor of 53BP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUbiquitin
TagsFlagExpressionMammalianMutationUbvG08 with I44A mutation and no terminal GlycinesPromoterCMVAvailable sinceJune 30, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pCAG-SpCas9-GFP-U6-gRNA
Plasmid#79144PurposeAll-in-one CRISPR/Cas9 vector with CAG promoter for expression in human ESC/iPSCDepositorInsertSpCas9
UseCRISPRTags2A-GFPExpressionMammalianPromoterCAGAvailable sinceJune 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBB Flag GCN5
Plasmid#74784Purposeexpress of GCN5 in mammalian cellsDepositorAvailable sinceJune 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMK294 (mAID-mCherry2-Bsr)
Plasmid#72832PurposeC-terminal tagging with mAID-mCherry2 using CRISPR/Cas9DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmAID-mCherry2-Bsr
UseCRISPRTagsmAID-mCherry2ExpressionMammalianAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Ornithine transcarbamylase (OTC)
Plasmid#71877PurposeFor mitochondrial unfolded protein response researchDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOTC (Otc Rat)
TagsNoneExpressionMammalianPromoterCAGAvailable sinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDH-PGK
Plasmid#72268PurposeExpress gene of interest under PGK (phosphoglycerate kinase I) promoterDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterPGKAvailable sinceJan. 11, 2016AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
GFP_CD47_LU
Plasmid#65474PurposeGFP tagged CD47 with its own long 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSignal peptide of CD47
GFP
CD47 coding region continued and long 3’UTR (with proximal polyadenylation signal changed from AAUAAA to ACUCAA).
ExpressionMammalianAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-PBRM1
Plasmid#65387PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPBRM1 (PBRM1 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-BRD3
Plasmid#65377PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBRD3 (BRD3 Human)
TagsEmGFP and V5ExpressionMammalianMutationnonePromoterCMVAvailable sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOTTC407 - pAAV EF1a eGFP
Plasmid#60058PurposeAn AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.DepositorInsertenhanced Green Fluorescent Protein
UseAAVExpressionMammalianPromoterEF1aAvailable sinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLentiCMV Puro DEST p38KTRmCerulean3
Plasmid#59155PurposeLentiviral vector to express p38 KTR mCerulean3 under CMV promoter (With Puromycin Resistance)DepositorInsertp38 Kinase Translocation Reporter (MAPK14 Mouse, Human)
UseLentiviralTagsmCerulean3ExpressionMammalianPromoterCMVAvailable sinceSept. 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Beta-Catenin-20
Plasmid#55001PurposeLocalization: Tight Junctions, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBeta-Catenin (Ctnnb1 Mouse)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn Con/Fon hChR2(H134R)-EYFP
Plasmid#55645PurposeCre-on/Flp-on ChR2-EYFP under the Synapsin promoterDepositorHas serviceAAV5, AAV8, AAV9, and AAV RetrogradeInsertChR2(H134R)-EYFP
UseAAV; Cre on/flp on chr2-eyfpTagsEYFPExpressionMammalianPromoterhSynAvailable sinceAug. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by