81,793 results
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pCMV4-p105
Plasmid#23288DepositorInsertp105 (NFKB1 Human)
ExpressionMammalianAvailable SinceApril 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3xFLAG-CMV10-hAtg13
Plasmid#22872DepositorAvailable SinceFeb. 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
phGluc
Plasmid#22522DepositorInsertGaussia luciferase
ExpressionMammalianAvailable SinceDec. 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-USP3
Plasmid#22582DepositorAvailable SinceNov. 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-AKT-PH[R25C]-GFP
Plasmid#18837DepositorAvailable SinceJuly 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag1-NS3
Plasmid#17645DepositorAvailable SinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pJ3M-Mst1 K59R
Plasmid#12204DepositorAvailable SinceAug. 11, 2006AvailabilityAcademic Institutions and Nonprofits only -
evoBxb1
Plasmid#222338PurposePlasmid for expressing evolved Bxb1 in the mammalian cellsDepositorInsertCodon-optimized evolved Bxb1
ExpressionMammalianPromoterCMVAvailable SinceJune 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PIEZO1-HaloTag
Plasmid#207834PurposePIEZO1 fusion for biochemistry and live imagingDepositorAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHage2-EF1a-TERT-IRES-Puro
Plasmid#182590Purposeexpresses TERT in mammalian cellsDepositorAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpG-P2A-EGFP (RTW4177)
Plasmid#139988PurposeCMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337RPromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLifeAct-HyPer7
Plasmid#136464PurposeMammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imagingDepositorInsertHyPer7
TagsLifeActExpressionMammalianPromoterCMVAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX458M-53BP1-DN1S
Plasmid#131045PurposePlasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.DepositorInsertSpCas9-53BP1-DN1S (TP53BP1 Human)
UseCRISPRTagsGFP and SpCas9ExpressionMammalianMutationOnly expressing residues 1231-1644 of complete 53…PromoterCBHAvailable SinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR
Plasmid#118154PurposeCatalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.DepositorInsertKRAB-dCas9-P2A-BlastR
UseCRISPR and LentiviralTagsHAExpressionMammalianMutationSee depositor comments belowPromoterEF1a core and U6Available SinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-sadCas9-VP64
Plasmid#115790PurposeAn AAV vector expressing S. aureus dCas9 fused to VP64.DepositorInsertSa-dCas9
UseAAVTagsNLS and NLS-VP64ExpressionMammalianPromoterCMVAvailable SinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (mCherry)
Plasmid#102945PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (mCherry)DepositorInsertSIINFEKL (OVAL Synthetic, Chicken)
UseRetroviralExpressionMammalianMutationOVA257-264PromoterLTRAvailable SinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXPR_047
Plasmid#107145Purposemeasures Cas9 activityDepositorInsertGFP + sgRNA for GFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceMarch 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo Fluc EGFP
Plasmid#90170PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteinsDepositorInsertFLuc EGFP
ExpressionMammalianAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucDM EGFP
Plasmid#90172PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutationDepositorInsertFLuc EGFP
ExpressionMammalianMutationR188Q, R261Q in FLucAvailable SinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only
