74,970 results

1. pBT281

   Plasmid

   #122611

   Purpose

   express evoAPOBEC1-BE4max in mammalian cells

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   evoAPOBEC1-BE4max
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   E4K H109N H122L D124N R154H A165S P201S F205S

   Promoter

   Available since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-ATF4 mScarlet NLS

   Plasmid

   #115969

   Purpose

   ER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I

   Depositor

   David Andrews

   Article

   Nougarede et al Assay Drug Dev Technol. 2018 Aug 8. doi:

   Insert

   activating transcription factor 4 (ATF4 Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   HA tag, c-myc NLS, and mScarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-sapphireCKAR

   Plasmid

   #118468

   Purpose

   cp-T-sapphire-based single-color biosensor for monitoring Protein Kinase C activity.

   Depositor

   Jin Zhang

   Article

   Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

   Insert

   sapphireCKAR
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

   Plasmid

   #90086

   Purpose

   This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Lyso-ExRai-AMPKAR

   Plasmid

   #192449

   Purpose

   ExRai-AMPKAR targeted to the lysosomal membrane.

   Depositor

   Jin Zhang

   Article

   Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

   Insert

   Lyso-ExRai-AMPKAR
   

   Use

   Tags

   Full-length LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFRT/TO/HIS/FLAG/HA-PGK1

   Plasmid

   #38071

   Depositor

   Markus Landthaler

   Article

   Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

   Insert

   PGK1 phosphoglycerate kinase 1 (PGK1 Human)
   

   Use

   Tags

   HIS/FLAG/HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF.hICN1.Ubc.GFP

   Plasmid

   #17626

   Depositor

   Linzhao Cheng

   Article

   Yu et al Stem Cells. 2006 Apr . 24(4):876-88.

   Insert

   ICN1 (NOTCH1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-SUN1_916

   Plasmid

   #125850

   Purpose

   Expresses SUN1_916 tagged with GFP in mammalian cells

   Depositor

   Miki Hieda

   Article

   Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

   Insert

   SUN1_916 (SUN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   stop codon

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. GPR161-Tango

    Plasmid

    #66334

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR161 (GPR161 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only