81,052 results

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AAVS1-Bxb1-LP-v2-TC

Plasmid

#194326

Purpose

AAVS1 targeting construct with the Bxb1 landing pad version 2 cassette.

Depositor

Richard Davis

Article

Blanch-Asensio et al Stem Cell Res. 2023 Feb;66:102991. doi:

Insert

loxP-EBFP2-attP-BleoR-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pSpCas9_T2A_iRFP670 sgAAVS1

Plasmid

#192477

Purpose

All-in-one plasmid for cutting non-targeting locus in DSB Spectrum V1, V2, or V3 cells as a control

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

SpCas9

Tags

T2A iRFP670

Expression

Mammalian

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-EGFP-caax

Plasmid

#190155

Purpose

Expresses membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Has Service

AAV1 and AAV8

Insert

EGFP-caax

Use

AAV

Expression

Mammalian

Promoter

MBP

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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ERK-KTR-mStrawberry reporter

Plasmid

#158686

Purpose

This plasmid is a ERK pathway reporter. It has CMV promoter driving the expression of ERK-KTR fused to mStrawberry-pGK-BSD

Depositor

Ravid Straussman

Article

Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

Insert

mStrawberry

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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PB-TO-NFIA.1-SOX9 (bi-directional promoter)

Plasmid

#182306

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into astrocytes via NFIA and SOX9 expression

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

NFIA (NFIA Human)
SOX9 (SOX9 Human)
puroR-T2A-mycNLS-mTagBFP2

Use

Piggybac

Tags

None and T2A-mycNLS-mTagBFP2

Expression

Mammalian

Promoter

TRE3G-Bi-directional

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA-BSD

Plasmid

#175583

Purpose

Expresses sgRNA for CasRx in mammalian cell

Depositor

Qi Xie

Article

Xia et al Nucleic Acids Res. 2021 Jul 21;49(13):73

Insert

sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Nov. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMRX-IPU-GFP-MYH9

Plasmid

#168273

Purpose

Expresses MYH9 in mammalian cells

Depositor

Noboru Mizushima

Article

Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

Insert

MYH9 (MYH9 Human)

Tags

FLAG

Expression

Mammalian

Available Since

June 8, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-HA-BACE1

Plasmid

#165032

Purpose

HA-BACE1

Depositor

Juan Bonifacino

Article

Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

Insert

BACE1 (BACE1 Human)

Tags

HA (embedded after propeptide cleavage site)

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-SFPQ

Plasmid

#166960

Purpose

Expresses FLAG-tagged SFPQ protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

SFPQ (SFPQ Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

Plasmid

#166015

Purpose

mammalian expression of HCoV-OC43 soluble spike trimer protein

Depositor

Dominic Esposito

Article

Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

Insert

HCoV-OC43 S (spike)

Tags

3C-His8-Strep2x2

Expression

Mammalian

Promoter

CMV51

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDY32 AAVS1-PuroR-9xTet0-minCMV-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#161928

Purpose

Donor plasmid for integrating minCMV promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

Surface marker and Citrine activation reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pJT039 AAVS1-PuroR-9xTet0-pEF-IGKleader-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#161927

Purpose

Donor plasmid for integrating pEF promoter-driven dual surface marker/fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

Surface marker and Citrine repression reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

pEF

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGIPz-mPD-L1

Plasmid

#121488

Purpose

Expression of mouse PD-L1 WT

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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Nrxn3b-Nav1.6-MKII-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

Plasmid

#163029

Purpose

FLiCRE TF for neuronal expression

Depositor

Alice Ting

Article

Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

Insert

Nrxn3b-MKII-hLOV1-TEVcs-tTA

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pEGBacmam-NGS Wnt-Fc

Plasmid

#159629

Purpose

Express Fc-tagged Fzd7/8 subtype NGS Wnt in mammalian cells for organoid research

Depositor

Chris Garcia

Article

Miao et al Cell Stem Cell. 2020 Aug 14. pii: S1934-

Insert

Fzd7/8 subtype NGS Wnt Fc

Tags

Fc tag and His tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG R2pH-LAMP1-3xFLAG

Plasmid

#157940

Purpose

expresses ratiometric sensor of lysosomal lumenal pH

Depositor

Massimiliano Stagi

Article

Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080

Insert

R2pH-LAMP1-3xFLAG (Lamp1 Mouse)

Tags

3XFLAG, mCherry, and pHluorin

Expression

Mammalian

Promoter

Chick beta actin pomoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-PspCas13b-gRNA-Actb1216

Plasmid

#155368

Purpose

PspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylation

Depositor

David Liu

Article

Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

Insert

PspCas13b gRNA targeting Actb 1216

Use

CRISPR

Expression

Mammalian

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pCSDest-HA-TMPRSS2

Plasmid

#154963

Purpose

TMPRSS2 with N-terminal HA

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

HA-TMPRSS2 (TMPRSS2 Human)

Tags

HA

Expression

Mammalian

Available Since

July 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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tet_pLKO.1_puro_shNRF2 #2

Plasmid

#136585

Purpose

Expresses an inducible short hairpin targeting human NRF2 sequence

Depositor

Kevin Janes

Article

Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

Insert

shNRF2v2 (NFE2L2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

June 13, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-mCherry-CDK4KTR

Plasmid

#126680

Purpose

Fluorescent reporter for CDK4/6 activity

Depositor

Hee Won Yang

Article

Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

Insert

RB cterm

Use

Lentiviral

Expression

Mammalian

Available Since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pHLS-EF1a-FKBP12-Gag(HIV)

Plasmid

#138476

Purpose

Expresses FKBP12 fused Gag for packaging FRB-SpCas9 into NanoMEDIC particle.

Depositor

Akitsu Hotta

Article

Gee et al Nat Commun. 2020 Mar 13;11(1):1334. doi:

Insert

HIV Gag

Tags

FRBP12 and Lynn Myristolation site

Expression

Mammalian

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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enAsABE8e

Plasmid

#138506

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(8e)-dAsCas12a (En)

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-mCh

Plasmid

#120169

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and mCherry (fluorescent tag)

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pBT280

Plasmid

#122610

Purpose

express evoFERNY-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoFERNY-BE4max

Expression

Mammalian

Mutation

H102P D104N

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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PB-gRNA-Puro

Plasmid

#121121

Purpose

Expression of a gRNA together with a Puromycin resistance

Depositor

Pablo Navarro

Article

Heurtier et al Nat Commun. 2019 Mar 7;10(1):1109. doi:

Insert

gRNA

Use

CRISPR

Expression

Mammalian

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCF525-EF1a-Hygro-P2A-mCherry-lenti

Plasmid

#115796

Purpose

Expresses mCherry in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Article

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

mCherry

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available Since

Oct. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-eYFP

Plasmid

#117382

Purpose

An AAV genome that expresses the fluorescent protein eYFP from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV_ABEmax_3xHA

Plasmid

#112098

Purpose

A:T-to-G:C base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

Insert

ABEmax_3xHA

Use

CRISPR

Tags

3xHA

Expression

Mammalian

Promoter

CMV

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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TATA-GFP

Plasmid

#110833

Purpose

negative control

Depositor

Michael Lewis

Article

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

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81,052 results