78,002 results

1. pWZL Blast myc

   Plasmid

   #10674

   Depositor

   William Hahn

   Article

   Boehm et al Mol Cell Biol. 2005 Aug . 25(15):6464-74

   Insert

   c-myc (MYC Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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2. GFP-PH-TAPP1

   Plasmid

   #161985

   Purpose

   Expresses GFP-tagged PH-Tapp1 in mammalian cells (PI(3,4)P2 biosensor)

   Depositor

   Dario Alessi, David Bryant

   Article

   Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

   Insert

   PH-TAPP1 (PLEKHA1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. iF53 PB-Zim3-dCas9-mScarlet-Hygro

   Plasmid

   #204726

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Expression

   Mammalian

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MEF2C-HA

   Plasmid

   #32515

   Depositor

   Andrew Lassar

   Article

   Kozhemyakina et al Mol Cell Biol. 2009 Nov;29(21):5751-62.

   Insert

   Mef2c (Mef2c Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSLQ2818 pPB: CAG-PYL1-KRAB-IRES-Puro-WPRE-SV40PA PGK-ABI-tagBFP-SpdCas9

   Plasmid

   #84241

   Purpose

   Expresses ABA-inducible KRAB-Sp dCas9

   Depositor

   Stanley Qi

   Article

   Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

   Inserts

   ABI-tagBFP-Sp dCas9
   PYL1-KRAB
   

   Use

   CRISPR and Synthetic Biology; Piggybac

   Tags

   2xNLS (SV40), ABI, HA tag, IRES-Puro, PYL1, and t…

   Expression

   Mammalian

   Promoter

   CAG and PGK

   Available Since

   Nov. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. Halo-TOMM20-N-10

   Plasmid

   #123284

   Purpose

   Localization: Outer Mitochondrial Membrane

   Depositor

   Kevin McGowan

   Insert

   TOMM20 (TOMM20 Human)
   

   Tags

   HaloTag7

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.GFAP.Cre.WPRE.hGH

   Plasmid

   #105550

   Purpose

   AAV expression of Cre from GFAP promoter

   Depositor

   James M. Wilson

   Has Service

   AAV PHP.eB and AAV5

   Insert

   Cre
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   May 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKS035 - pCAGGS-3XFLAG-Rad21-mKate2-bpA

   Plasmid

   #156445

   Purpose

   For transient expression of mouse RAD21 (SCC1) tagged with mKate2

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   mouse RAD21 (Rad21 Mouse)
   

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGGS-synPCB2.1

   Plasmid

   #139478

   Purpose

   Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

   Depositor

   Kazuhiro Aoki

   Article

   Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

   Insert

   MTS-Fnr-Fd-P2A-MTS-PcyA-FLAG-P2A-MTS-HA-HO1
   

   Expression

   Mammalian

   Promoter

   CAG promoter

   Available Since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. DRD2-Tango

    Plasmid

    #66269

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    DRD2 (DRD2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMax_mOrange

    Plasmid

    #177827

    Purpose

    Host Cell Reactivation (HCR) control mOrange plasmid (pMax backbone)

    Depositor

    Leona Samson, Zachary Nagel

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFH3.3_Cre-MS2x12

    Plasmid

    #205559

    Purpose

    Express Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Cre
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CMV-synaptophysin-Lime

    Plasmid

    #201016

    Purpose

    Expresses Lime in synaptic vesicle lumen by fusion to synaptophysin

    Depositor

    Jaime de Juan-Sanz

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Synaptophysin-Lime (Syp Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. iRFP670-PIP4K2C

    Plasmid

    #202750

    Purpose

    expresses fluorescent protein-tagged enzyme

    Depositor

    Gerry Hammond

    Article

    Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

    Insert

    iRFP670:PIP4K2C (PIP4K2C Human)
    

    Expression

    Mammalian

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCB6 ZO1myc

    Plasmid

    #30317

    Depositor

    James Anderson, Alan Fanning

    Article

    Fanning et al J Biol Chem. 1998 Nov 6. 273(45):29745-5

    Insert

    Zonula Occludens-1 (TJP1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    none

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

16. eMscL WT

    Plasmid

    #107454

    Purpose

    eMscL WT is optimized for mammalian rodent neuronal expression to the plasma membrane through a neuron-specific promoter and a voltage-gated channel targeting motif

    Depositor

    Francesco Difato

    Article

    Soloperto et al J Cell Sci. 2018 Jan 29. pii: jcs.210393

    Insert

    bacterial mechanosensitive ion channel of large conductance (mscL Escherichia Coli)
    

    Use

    Adeno-associated viral

    Tags

    Kir2.1 ER export signal (FCYENEV) and tdTomato fl…

    Expression

    Mammalian

    Promoter

    human synapsin 1

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PE6e

    Plasmid

    #207855

    Purpose

    Mammalian expression of PE6e prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6e
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Cas9(H840A, K918A, K775R)

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT-TODestFLAGHA_HuB

    Plasmid

    #65755

    Purpose

    expresses FLAGHA-tagged HuB in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL2 (ELAVL2 Human)
    

    Tags

    FLAG HA and V5

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. HTR1A-Tango

    Plasmid

    #66404

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    HTR1A (HTR1A Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3 HA-humanTRAF2 (1084)

    Plasmid

    #66932

    Purpose

    Mammalian Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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