82,530 results
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Plasmid#106249PurposeLentiviral vector with puro selection for expression of S. aureus dCas9-KRABDepositorInsertdead S. aureus Cas9 KRAB T2A PuroR
UseCRISPR and LentiviralTagsHA-NLS and NLS-KRABExpressionMammalianMutationD10A and N580APromoterhUbCAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX551-CMV-SpCas9
Plasmid#107024PurposeAAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang labDepositorInsertSpCas9
UseAAV and CRISPRExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENN.AAV.CamKII.GCaMP6f.WPRE.SV40
Plasmid#100834PurposeAAV expression of ultrasensitive protein calcium sensor from CamKII promoterDepositorHas serviceAAV1, AAV5, and AAV9InsertGCaMP6f
UseAAVExpressionMammalianMutationGCaMP3-T302L R303P A317E D380Y T381R S383T R392GPromoterCamKIIAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.GfaABC1D.PI.Lck-GFP.SV40
Plasmid#105598PurposeAAV expression of membrane bound EGFP from GfaABC1D promoterDepositorPublicationHas serviceAAV5InsertLck-EGFP
UseAAVExpressionMammalianPromoterGfaABC1DAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pTRIP-CMV-Puro-2A-cGAS
Plasmid#102612PurposeLentivector expressing cGASDepositorAvailable sinceJan. 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pC0053-CMV-dPspCas13b-GS-ADAR2DD(E488Q)-delta-984-1090
Plasmid#103869PurposedPspCas13b-ADAR2DD(E488Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAVDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsUseCRISPRTagsGS and HIV NESExpressionMammalianMutationChanged histidne 133 to alanine and histidine 105…Available sinceNov. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VN-Tau (P301L)
Plasmid#87634Purposeexpresses hTau fused to the N-terminal part of VenusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAPT (MAPT Human)
TagsN-terminal part of VenusExpressionMammalianMutationMutation P301LPromoterCMVAvailable sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiGuide-Hygro-iRFP670
Plasmid#99377PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Hygro-P2A-iRFP670
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1a and hU6Available sinceAug. 18, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-TRE-mRuby2
Plasmid#99114PurposeAn AAV genome with tet-inducible expression of the fluorescent protein mRuby2DepositorInsertmRuby2
UseAAVExpressionMammalianPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AICSDP-23:TJP1-mEGFP
Plasmid#87429PurposeHomology arms and linker-mEGFP sequence for N-terminus tagging of human TJP1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTJP1 Homology Arms with mEGFP-linker (TJP1 Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …Available sinceMarch 21, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
Hygro-Cas9 donor
Plasmid#86883PurposeDonor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus with hygromycin selectionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthSpCas9
UseCRISPRTags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianAvailable sinceMarch 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
human MMP-9 promoter
Plasmid#53434Purposepromoter activity assayDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMMP-9 promoter (MMP9 Human)
UseLuciferaseTagsluciferaseExpressionMammalianPromoterMMP9Available sinceFeb. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDisplay-HRP-KDEL
Plasmid#85582PurposeExpresses HRP-KDEL in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHRP-KDEL
TagsV5ExpressionMammalianAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJL-SaBE3
Plasmid#85169PurposeExpresses SaBE3 in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSaBE3
ExpressionMammalianPromoterCMVAvailable sinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmScarlet-i_alphaTubulin_C1
Plasmid#85047PurposeIn vivo visualization of microtubules (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAlpha-Tubulin (TUBA1B Human)
TagsmScarlet-iExpressionMammalianPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRE5
Plasmid#62377PurposeFluorescent reporter to quantify the ratio of two alternative splicing patterns.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdsRed and GFP
ExpressionMammalianAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PA-Cdc42
Plasmid#75263PurposeLight-activated Cdc42DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCell Division Cycle 42 (CDC42 Human)
Tags6xHIs, LOV2, and mVenusExpressionBacterial, Insect, and Mamm…MutationF56WPromoterCMVAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry
Plasmid#75470PurposeFlp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cellsDepositorHas serviceAAV1InsertReversed ChR2(H134R)-mCherry
UseAAV and Synthetic BiologyExpressionMammalianPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
jk100 pSinEGdsp(p1:MAPPnL;p2:gfp:barcodelandingpad:4xboxB)
Plasmid#79785Purposegenomic Sindbis plasmid, encoding MAPPnL and a GFP RNA with a barcode landing padDepositorInsertMAPP-nL
UseSynthetic Biology; Sindbis virus genomic plasmidExpressionMammalianAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1_SEPT2
Plasmid#71547Purposemammalian expression of Sept 2 with a GFP fusionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSeptin 2 (Septin2 Mouse)
TagseGFPExpressionMammalianPromoterCMVAvailable sinceApril 1, 2016AvailabilityAcademic Institutions and Nonprofits only