80,773 results

Showing: 6501 - 6520 of 80773 results

Results per page:

Sort results by:

mEGFP-HRas G12V

Plasmid

#18666

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Tags

monomeric EGFP

Expression

Mammalian

Mutation

G12V mutation in HRas

Available Since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0

Plasmid

#174081

Purpose

Expression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.

Depositor

Ryan Mehl

Article

Jang et al J Am Chem Soc. 2020 Apr 22;142(16):7245-

Insert

NES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0

Tags

NES-Flag

Expression

Mammalian

Mutation

L270G, Y271L, N311G, C313A

Promoter

CMV

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1 PRDM16

Plasmid

#15503

Depositor

Bruce Spiegelman

Article

Seale et al Cell Metab. 2007 Jul . 6(1):38-54.

Insert

PRDM16 (Prdm16 Mouse)

Expression

Mammalian

Available Since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV(MinDis).iGluSnFR

Plasmid

#41732

Purpose

Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

Insert

GltI based Glutamate Biosensor

Use

Glutamate biosensor

Tags

Myc, PDGFR transmembrane helix (aa 513-561), and …

Expression

Mammalian

Mutation

cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

Promoter

CMV

Available Since

Jan. 3, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
YFP-CL1

Plasmid

#11950

Depositor

Nico Dantuma

Article

Menendez-Benito et al Hum Mol Genet. 2005 Oct 1. 14(19):2787-9

Insert

YFP-CL1

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
NLS-Laconic/pCMV-myc-nuc

Plasmid

#46307

Purpose

a nuclear-targeted, genetically-encoded sensor for lactate

Depositor

Luis Felipe Barros

Article

Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

Insert

NLS-Laconic

Use

Lactate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-puro U6 sgRNA BfuAI stuffer

Plasmid

#50920

Purpose

U6 driven sgRNA cloning vector where guide sequences are inserted between BfuAI sites

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXs-Nanog

Plasmid

#13354

Purpose

Retroviral expression of mouse Nanog

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

Nanog homeobox (Nanog Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
AbVec_IGHC

Plasmid

#183701

Purpose

Encode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.

Depositor

David Nemazee

Insert

IGHG1 (IGHG1 Human)

Expression

Mammalian

Promoter

SP6

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
miniCMV eGFP hPGK BSD

Plasmid

#188519

Purpose

Backbone for CRISPR activation (CRISPRa) isogenic PAM testing

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Type

Empty backbone

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLARRYv2-T-Sapphire

Plasmid

#233209

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with T-Sapphire marker

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pWN_U6-B2M-miniGag

Plasmid

#228957

Purpose

miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag

Expression

Mammalian

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti SYN-FLAG-dCas9-VPR

Plasmid

#114196

Purpose

Lentivirus compatible dCas9-VPR construct driven by the human SYN promoter

Depositor

Jeremy Day

Article

Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

Insert

dCas9-VPR

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SYN

Available Since

Sept. 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFPN3-Sstr3

Plasmid

#35623

Depositor

Kirk Mykytyn

Article

Berbari et al Mol Biol Cell. 2008 Apr;19(4):1540-7. Ep

Insert

Sstr3 (Sstr3 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES-Ap2a2-FKBP

Plasmid

#46944

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-2 alpha subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Article

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap2a2-FKBP

Tags

Mainly mouse Ap2a2 cDNA, with a fragment of brain…

Expression

Mammalian

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3 Flag WDR5

Plasmid

#15552

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Wdr5 (Wdr5 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-VEGFR2-mCherry

Plasmid

#139606

Purpose

Expresses a VEGFR2-mCherry fusion protein in mammalian cells

Depositor

Michael Welsh

Article

Pietila et al Cells. 2019 Dec 15;8(12). pii: cells8121

Insert

KDR (KDR Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-Myc-GFP

Plasmid

#83375

Purpose

Expression of Myc tagged GFP

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

Myc-GFP

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-N1-Cdc45

Plasmid

#211426

Purpose

mammalian expression of human Cdc45 C-terminally fused to EGFP

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
(559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

Plasmid

#163024

Purpose

Expression of CRISPRi with U6-gRNA (Bsa1 sites)

Depositor

Tonia Rex

Article

Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

Insert

Mammalian codon-optimized SadCas9

Use

AAV

Tags

KRAB, SV40 polyA, and myc NLS

Expression

Mammalian

Promoter

hEF-1a

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge