78,036 results

1. pBVboostFGII WPRE 2A-CVB3

   Plasmid

   #202076

   Purpose

   Bacterial expression of 2A protease from coxsackievirus B3 (CVB3). Contains C-terminal His6-tag.

   Depositor

   Vesa Hytönen

   Article

   Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

   Insert

   CVB3 2A protease with HisTag
   

   Tags

   6xHis-tag

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   polh, CAG, T7

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDx_mCherry-SYFP2

   Plasmid

   #189759

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mCherry-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mCherry-SYFP2
   

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Promoter

   CMV & Rha

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SFRS10

   Plasmid

   #156007

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   SFRS10 (TRA2B Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-Halo,IRES-NGFR

   Plasmid

   #164520

   Purpose

   All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

   Depositor

   Mitchell Guttman

   Article

   Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLEX-uORF-HA-birA*-N-Ras(Q61K/T35A)-IRES-Puro

   Plasmid

   #120565

   Purpose

   Expresses HA-birA*-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, T35A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLEX-uORF-HA-birA*-N-Ras(Q61K/Y40A)-IRES-Puro

   Plasmid

   #120567

   Purpose

   Expresses HA-birA*-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, Y40A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX-uORF-HA-birA*-N-Ras(Q61K/E37A)-IRES-Puro

   Plasmid

   #120566

   Purpose

   Expresses HA-birA*-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

   Depositor

   Paul Khavari

   Article

   Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

   Insert

   NRAS (NRAS Human)
   

   Use

   Lentiviral

   Tags

   HA-birA*

   Expression

   Mammalian

   Mutation

   Q61K, E37A

   Promoter

   CMV

   Available Since

   Jan. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Xl-VSP1 R152Q

   Plasmid

   #51881

   Purpose

   expression of Xl-VSP1 mutant in Xenopus oocytes or mammalian cells

   Depositor

   Laurinda Jaffe

   Article

   Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

   Insert

   VSP1 R152Q (tpte2 Frog)
   

   Use

   Xenopus oocyte expression

   Expression

   Mammalian

   Mutation

   R152Q: near the extracellular side of the fourth …

   Promoter

   CMV

   Available Since

   May 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUB-GFP

   Plasmid

   #11155

   Purpose

   Mammalian expression vector for expression of GFP (Ubiquitin C promoter)

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

   Has Service

   Cloning Grade DNA

   Insert

   Ubiquitin C promoter (UBC Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 25, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHCMV-10A1

    Plasmid

    #15805

    Depositor

    Miguel Sena-Esteves

    Article

    Sena-Esteves et al J Virol Methods. 2004 Dec 15. 122(2):131

    Insert

    10A1 Murine leukemia virus envelope mRNA
    

    Expression

    Mammalian

    Available Since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti SpBsmBI sgRNA Puro

    Plasmid

    #62207

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. EGFP-hAgo2

    Plasmid

    #21981

    Depositor

    Phil Sharp

    Article

    Leung et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

    Insert

    Argonaute 2 (AGO2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. AbVec2.0-IGHG4

    Plasmid

    #99578

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotype

    Depositor

    Hedda Wardemann

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-C-6His-mEsrra

    Plasmid

    #173152

    Purpose

    mouse estrogen-related receptor alpha (Esrra) expression plasmid in mammalian cells

    Depositor

    Brijesh Kumar Singh

    Article

    Tripathi et al bioRxiv 2021.11.16.468891

    Insert

    ORF clone Estrogen-related receptor alpha (Esrra) (Esrra Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Mutation

    Wild type

    Available Since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. CBF:H2B-Venus

    Plasmid

    #44211

    Depositor

    Anna-Katerina Hadjantonakis

    Article

    Nowotschin et al BMC Dev Biol. 2013 Apr 25;13:15. doi: 10

    Insert

    CBF1-Responsive-Element
    

    Expression

    Mammalian

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-EF1a-BbChT

    Plasmid

    #45186

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-hSyn-nucGFP

    Plasmid

    #140190

    Purpose

    Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

    Depositor

    Lorenz Studer

    Article

    Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

    Insert

    GFP-NLS
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLifeAct-mTurquoise2

    Plasmid

    #36201

    Purpose

    In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    LifeAct
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-CMV 3xFLAG-LC3B-GFP

    Plasmid

    #123107

    Purpose

    Expresses 3xFLAG-LC3B-EGFP in mammalian cells, for monitoring ATG4 activity towards LC3B

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    3xFLAG and EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. GFP-PIK3C2A

    Plasmid

    #161988

    Purpose

    Mammalian expression vector containing PIK3C2A tagged with GFP

    Depositor

    David Bryant, Volker Haucke

    Article

    Posor et al Nature. 2013 Jul 11;499(7457):233-7. doi

    Insert

    PIK3C2A (PIK3C2A Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Dec. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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