78,868 results

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pcDNA3.1 myc-RNF114

Plasmid

#58295

Purpose

mammalian expression of myc tagged RNF114

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Tags

his and myc

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
CROPseq-multi-EBFP2-NLS (pRW1447)

Plasmid

#216219

Purpose

Entry vector to clone sgRNA(s) into the CROPseq-multi construct with nuclear-localized EBFP2 expression

Depositor

Paul Blainey

Article

Walton et al bioRxiv 2024.03.17.585235

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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tdTomato-TOMM20-N-10

Plasmid

#58137

Purpose

Localization: Outer Mitochondrial Membrane, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

TOMM20 (TOMM20 Human)

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only
pLEX-FLAG-NRAS-IRES-Puro

Plasmid

#120574

Purpose

Expresses Flag-N-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

NRAS (NRAS Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-CDK4

Plasmid

#172612

Purpose

Expresses HA-tagged CDK4 in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CDK4 (CDK4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-nuc-GsCT

Plasmid

#205517

Purpose

Nuclear-targeted C-terminal fragment of human Gαs protein (Gαs inhibitory peptide).

Depositor

Jin Zhang

Article

Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

Insert

nuc-GsCT (GNAS Human, Synthetic)

Tags

Histone H2A and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

Plasmid

#42333

Purpose

This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

Depositor

Feng Zhang

Article

Cong et al Science. 2013 Jan 3.

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

HA

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-eNme2-T.1-ABE8e

Plasmid

#185668

Purpose

Expresses eNme2-T.1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-neNme2-T.2

Use

CRISPR

Expression

Mammalian

Mutation

Nme2Cas9 D16A/E47K/V68M/T123A/D152G/E154K/T396A/H…

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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Lyso-GA

Plasmid

#209874

Purpose

Dimerization dependent fluorescent protein GA anchored to cytosolic face of the lysosomal membrane with human LAMP1

Depositor

Sarah Cohen

Article

Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25

Insert

ddGFP A

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-anti-mCherry(LaM6) PAGER(Gq)

Plasmid

#230020

Purpose

AAV vector expressing anti-mCherry PAGER(Gq) in neurons

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaM6-TEVcs-ALFA-hM4D

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pEF6a-CD28-PafA

Plasmid

#113400

Purpose

Expresses CD28-PafA in mammalian cell

Depositor

Min Zhuang

Article

Liu et al Nat Methods. 2018 Aug 13. pii: 10.1038/s

Insert

CD28-PafA

Tags

myc

Expression

Mammalian

Promoter

EF-1α

Available Since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pFUW-tetO-Meis1

Plasmid

#139824

Purpose

doxycycline-inducible expression of mouse Meis in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2018 Dec 7;3(30). pii: 3/30

Insert

Meis1 (Meis1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TRE-mCMV

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-ZO-2

Plasmid

#27422

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Available Since

April 15, 2011

Availability

Academic Institutions and Nonprofits only
p3662 HPV-16 E2

Plasmid

#22457

Depositor

Peter Howley

Article

Sakai et al J Virol. 1996 Mar . 70(3):1602-11.

Insert

E2 WT

Expression

Mammalian

Available Since

Nov. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pCI-MS2V5-PABPC1

Plasmid

#65807

Purpose

expression of PABPC1-MS2 fusion. MS2 tethers PABPC1 to mRNAs containing MS2 stem loops.

Depositor

Niels Gehring

Article

Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

Insert

PAB1 (PABPC1 Human)

Tags

MS2 stem loops and V5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-ATG3 WT

Plasmid

#129296

Purpose

Expresses 3xFLAG-ATG3 WT in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-ZDHHC17

Plasmid

#231665

Purpose

Expresses human ZDHHC17protein

Depositor

Chunhong Ma

Article

Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

Insert

ZDHHC17 (ZDHHC17 Human)

Tags

3×Flag Tag

Expression

Mammalian

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-LATS2

Plasmid

#172987

Purpose

constitutive expression of FLAG-tagged LATS2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-LATS2 (LATS2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pT2-shP53

Plasmid

#124261

Purpose

Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

Depositor

Maria Castro

Article

Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

Insert

shP53

Expression

Mammalian

Available Since

April 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-IP-PEX3-EGFP

Plasmid

#228142

Purpose

Expresses PEX3-GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

Insert

PEX3 (PEX3 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-14-3-3 ϵ

Plasmid

#48797

Purpose

expresses human myc-14-3-3ϵ in mammalian cells

Depositor

Huda Zoghbi

Article

Chen et al Cell. 2003 May 16;113(4):457-68.

Insert

YWHAE 14-3-3 ϵ (YWHAE Human)

Tags

haemagglutinin

Expression

Mammalian

Promoter

cmv

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-20nm-EBFP2

Plasmid

#231185

Purpose

Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 20 nm

Depositor

Dmitry Lim

Article

Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

Insert

20nm-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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VAMP3-mCherry

Plasmid

#92423

Purpose

Expression of VAMP3 C-terminally conjugated to mCherry.

Depositor

Geert van den Bogaart

Article

Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

Insert

VAMP3 (Vamp3 Mouse)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Myc shRNA

Plasmid

#15662

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Myc shRNA (MYC Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

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sgRNA_expression_vector

Plasmid

#210212

Purpose

An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

Depositor

Albert Jeltsch

Article

Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

Type

Empty backbone

Expression

Bacterial and Mammalian

Promoter

U6

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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SV40-eGFP-Z1

Plasmid

#127638

Purpose

Minimal backbone plasmid for mammalian eGFP expression from SV40 promoter with zeocin/bleocin bacterial resistance

Depositor

Jordan Green

Insert

eGFP

Expression

Mammalian

Promoter

SV40

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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AP2u2-mCherry

Plasmid

#27672

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Ap2m1 (Ap2m1 Rat)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-VP4SA11

Plasmid

#89165

Purpose

Express Rotavirus SA11 VP4 in mammalian cells

Depositor

Takeshi Kobayashi

Article

Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

Insert

Rotavirus SA11 VP4

Expression

Mammalian

Promoter

T7 promotor

Available Since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Tfap2c

Plasmid

#70269

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2c

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

transcription factor AP-2, gamma (Tfap2c Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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CYFIP1.mCherry

Plasmid

#122051

Purpose

Expresses human CYFIP1 in mammalian cells.

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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78,868 results