82,530 results
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Plasmid#92195PurposeLentiviral overexpression vector to make stable bicistronic cell line for α-Syn screenDepositorInsertDsRed-IRES-SNCA-EGFP
UseLentiviralExpressionMammalianAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-mTurquoise2
Plasmid#99125PurposeAn AAV genome that expresses the fluorescent protein mTurquoise2 from the hSyn1 promoterDepositorInsertmTurquoise2
UseAAVExpressionMammalianPromoterhSyn1Available sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEGFP-PI35P2
Plasmid#92419PurposeFluorescent reporter for phosphatidylinositol (3,5)-bisphosphate (PI(3,5)P2). Mouse MCOLN1 residues 1–68 fused to eGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMCOLN1 (Mcoln1 Mouse)
TagseGFPExpressionMammalianMutationSynthetic gene, codon optimized for human.PromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCDNA5-Flag-BRD4-BD
Plasmid#90005Purposeexpresses BD mutant BRD4DepositorAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-Blimp1
Plasmid#73995PurposePlasmid expressing mouse Blimp1DepositorAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAIP hIL2 co
Plasmid#90513PurposeLentiviral vector for expression of human codon optimized IL2 with puromycin resistance.DepositorAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDUAL SRBI (GFP)
Plasmid#86979PurposeLentiviral expression construct encoding SR-B1 and GFP from separate promotersDepositorAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lenti-LbCpf1-Blast
Plasmid#84751PurposeExpresses human codon-optimized LbCpf1 protein and blasticidin resistance from EFS promoter. Lentiviral backbone.DepositorInsertLbCpf1
UseCRISPR and LentiviralTagsNLS-3xHA (C terminal on insert)ExpressionMammalianPromoterEFS-NSAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)
Plasmid#69138PurposeFor the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
TagsnoneExpressionMammalianAvailable sinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNTI194
Plasmid#84266PurposeExpresses Avi-tagged RPL1 and humanized birADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsTagsAvi tag, Flag, Gly/Ser linker, and TEV siteExpressionMammalianMutationR33A, A34E (*see below)PromoterCMV and P2A peptideAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-CASI-Cas9C-P2A-turboGFP
Plasmid#80942PurposeExpresses Cas9C in mammalian cells; co-expresses turboGFP.DepositorInsertCas9C
UseAAV, CRISPR, and Synthetic BiologyExpressionMammalianAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-Zdk1
Plasmid#81047Purposemammalian expression of Zdk1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertZdk1
Tags6x His tag, FLAG, and mVenusExpressionBacterial, Insect, and Mamm…Available sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMito-miRFP703
Plasmid#80000PurposeMitochondria labeling with near-infrared fluorescent protein miRFP703DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcytochrome C oxidase subunit VIII (COX8A Human)
TagsmiRFP703ExpressionMammalianPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGCaMP6m-N3-TPC2
Plasmid#80147PurposeGCaMP6 is a genetically encoded fluorescent Ca2+ sensor . TPC2-GCaMP6 allows the visualization of Ca2+ release from intracellular Ca2+ stores in real timeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTPCN2 (TPCN2 Human)
TagsGCaMP6mExpressionMammalianPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCIP
Plasmid#79009PurposeFor expression in mouse ESCs or other eukaryotic cells. Contains pCAGGS-IRES-Puro-bpA, with a NheI-MfeI cloning site in between pCAGGS and IRES.DepositorTypeEmpty backboneExpressionMammalianPromoterpCAGGsAvailable sinceJuly 5, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Synapsin sHRPa-NRX in FSW lentiviral vector
Plasmid#73147PurposeLarge fragment of split HRP fused to the extracellular terminus of neurexin3B. This is a lentiviral plasmid, but can also be used for transfections. Synapsin promoter.DepositorInsertsHRPa-NRX
UseLentiviralTags2x HA tag and Flag tagExpressionMammalianPromoterSynapsinAvailable sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG sHRPb-NLG
Plasmid#73144PurposeSmall fragment of split HRP fused to the extracellular terminus of NLG1. CAG promoter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsHRPb-NLG (Nlgn1 Mouse, Synthetic)
TagsV5 tagExpressionMammalianPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cytosolic FAK biosensor
Plasmid#78300PurposeFRET biosensor. Visualization of cytosolic FAK activity in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCytosolic FAK biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Lyn FAK biosensor
Plasmid#78299PurposeFRET biosensor. Visualization of FAK activity at lipid rafts in live cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn Fak biosensor
ExpressionMammalianPromoterCMVAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRubiC-T2A-Cas9
Plasmid#75347PurposeUbiquitin promoter expresses mCherry and humanized spCas9 (PX330) via a T2A motif. For cell transfection or use in retroviral (MMuLV) packaging. Use Pac1 and/or BstB1 sites to insert sgRNAs from PXL.DepositorInsertCas9
UseCRISPR and RetroviralTagsmCherry (via t2a)ExpressionMammalianPromoterUbiquitinAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only