78,043 results

1. siRNA-resistant mNG-ORP8

   Plasmid

   #195170

   Purpose

   mammalian expression of siRNA-resistant ORP8 tagged with mNeonGreen

   Depositor

   Gia Voeltz

   Article

   Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

   Insert

   ORP8 (OSBPL8 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Wobble mutations for siRNA resistance from K174 t…

   Promoter

   CMV

   Available Since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. gRNA-MS2x2-TagRFP657

   Plasmid

   #184441

   Purpose

   sgRNA with MS2 aptamer, TagRFP657 as selection marker

   Depositor

   Rhys Allan

   Article

   Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

   Insert

   sgRNA-MS2x2, TagRFP657
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHH21-BNA-Luc

   Plasmid

   #187924

   Purpose

   Expresses Luciferase reporter RNA construct under the 5' and 3' UTRs of NA segment of Influenza B/Brisbane/60/2008 virus by RNA polymerase I in mammalian cells

   Depositor

   Arindam Mondal

   Article

   Kedia et al Front Microbiol. 2022 May 25;13:868367.

   Insert

   Reporter RNA (RNA encoding firefly luciferase under the viral promoter of segment 6 of Influenza B/Brisbane/60/2008 virus)
   

   Expression

   Mammalian

   Promoter

   RNA polymease I Promoter

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-PE2-SpG

   Plasmid

   #159978

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

   Insert

   PE2-SpG
   

   Tags

   BpSV40 NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHCMV_CHIKV_E1E2

   Plasmid

   #207261

   Purpose

   Mammalian expression of Chikungunya virus E1E2 protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   CHIKV_E1E2 (CHIKVgp2 Chikungunya virus)
   

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available Since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-J chain

   Plasmid

   #145146

   Purpose

   Mammalian expression plasmid for J chain of polymeric antibody classes

   Depositor

   Erik Procko

   Article

   Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

   Insert

   immunoglobulin J chain (JCHAIN Human)
   

   Expression

   Mammalian

   Mutation

   Codon-optimized for human cell expression.

   Promoter

   CMV

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-APOBEC1-YTHmut

   Plasmid

   #131637

   Purpose

   Expresses APOBEC1 fused to a mutant YTH domain with diminished m6A binding

   Depositor

   Kate Meyer

   Article

   Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

   Insert

   APOBEC1-YTHmut-HA (YTHDF2 Rat, Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   YTH domain lacks AA 385-409 comprising the m6A-bi…

   Promoter

   CMV

   Available Since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mCherry KNL1 wt hardened

   Plasmid

   #45224

   Depositor

   Iain Cheeseman, Michael Lampson

   Article

   Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

   Insert

   mCherry KNL1 resistant to KNL1 siRNA (KNL1 Human)
   

   Tags

   TEV S15

   Expression

   Mammalian

   Available Since

   June 10, 2013

   Availability

   Academic Institutions and Nonprofits only

9. pLV-mTurquoise2-IL-1beta-mNeonGreen

   Plasmid

   #166783

   Purpose

   FRET analysis (pro-IL-1beta cleavage) in living cells

   Depositor

   Hao Wu

   Article

   Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

   Insert

   mTurquoise2-IL-1b-mNeongreen (IL1B Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen and mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

    Plasmid

    #139446

    Purpose

    Lentiviral vector genome to ectopically express Firefly luciferase

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    FLAG-tag Firefly luciferase
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9n(BB) (PX460)

    Plasmid

    #48873

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Has Service

    Cloning Grade DNA

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRNA-CleanCap-NanoLuc

    Plasmid

    #188247

    Purpose

    Template plasmid to transcribe mRNA encoding NanoLuc

    Depositor

    Howard Chang

    Article

    Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

    Insert

    NanoLuc
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Sept. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. TLCV2-APOBEC1-YTHmut

    Plasmid

    #178950

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTHmut-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Promoter

    tight TRE

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-hSyn-hChR2(H134R)-mCherry

    Plasmid

    #26976

    Purpose

    AAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV5, AAV8, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    hSyn

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pC13N-iCAG.copGFP

    Plasmid

    #66578

    Purpose

    CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs

    Depositor

    Jizhong Zou

    Article

    Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

    Insert

    copGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSP2-48-merTetO-EFS-BLaR

    Plasmid

    #118712

    Purpose

    Contains an optimized 48-mer TetO repeat for imaging of desired loci

    Depositor

    Huimin Zhao

    Article

    Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

    Inserts

    Blasticidin resistance gene
    EFS-NS promoter
    

    Expression

    Mammalian

    Promoter

    EFS-NS

    Available Since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. SuExp-hPLD3

    Plasmid

    #201243

    Purpose

    express full length human PLD3 protein

    Depositor

    David Nemazee

    Article

    Yuan et al Structure. 2024 Jun 6;32(6):766-779.e7.

    Insert

    PLD3 (PLD3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. FAS promoter luciferase

    Plasmid

    #8890

    Depositor

    Bruce Spiegelman

    Article

    Kim et al J Clin Invest. 1998 Jan 1. 101(1):1-9.

    Insert

    Fatty Acid Synthetase Promoter (Fasn Rat, unknown - rat or mouse)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    Nov. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. phage UbiC tagRFP-T-DDX6

    Plasmid

    #119947

    Purpose

    Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    DDX6 (DDX6 Human)
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK462 (Hygro-P2A-mAB)

    Plasmid

    #214388

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Hygro-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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