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82,530 results

Showing: 7541 - 7560 of 82530 results

  8. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid
    #117687
    Purpose
    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.
    Depositor
    Erik Sontheimer
    Publication
    Gao et al Methods Enzymol. 2019;616:365-383. doi:
    Inserts
    Kanamycin cassette
    TetR-P2A-BFP
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and hPGK
    Available since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    117687-plasmid-map-sequence-id-228342

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  10. pEJS654 All-in-One AAV-sgRNA-hNmeCas9

    Plasmid
    #112139
    Purpose
    Delivery of human-codon-optimized Cas9 from Neisseria meningitidis (NmeCas9) and its single-guide RNA in a single AAV vector for in vivo genome editing.
    Depositor
    Erik Sontheimer
    Publication
    Ibraheim et al Genome Biol. 2018 Sep 19;19(1):137. doi:
    Inserts
    sgRNA scaffold
    Human-codon-optimized NmeCas9
    Use
    AAV, CRISPR, and Mouse Targeting
    Expression
    Mammalian
    Promoter
    U1a and U6
    Available since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112139-plasmid-map-sequence-id-217823

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  15. pEGFPC1-hVAP-A KD/MD

    Plasmid
    #104449
    Purpose
    Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells
    Depositor
    Catherine Tomasetto
    Publication
    Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    vesicle-associated membrane protein-associated protein A (VAPA Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    changed K94 to D and M96 to D
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104449-plasmid-map-sequence-id-201409

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Showing: 7541 - 7560 of 82530 results