78,128 results

1. DD-Cas9 with filler sequence and Cre-ERT2 (EDCICE)

   Plasmid

   #90086

   Purpose

   This plasmid contains destabilized Cas9 and has Cre-ERT2 after IRES sequence. This vector also contains filler sequence which required to be removed for cloning of desired sgRNA

   Depositor

   Raffaella Sordella

   Article

   Senturk et al Nat Commun. 2017 Feb 22;8:14370. doi: 10

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Destabilized Domain and Flag

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. V5-APEX2-LMNA

   Plasmid

   #129275

   Purpose

   Expression of APEX2 at nuclear lamina

   Depositor

   Alice Ting

   Article

   Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

   Insert

   V5-APEX2-LMNA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CAG-DIO-HA-HCARD-P2A-mCitrine

   Plasmid

   #228902

   Purpose

   For AAV packaging and Cre-dependent expression of a peripherally restricted DREADD

   Depositor

   Bryan Roth

   Article

   Kang et al Cell. 2024 Dec 26;187(26):7433-7449.e20.

   Has Service

   AAV8

   Insert

   hydroxycarboxylic acid receptor 2 (Hcar2 Mouse)
   

   Use

   AAV and Synthetic Biology

   Tags

   HA and P2A mCitrine

   Expression

   Mammalian

   Mutation

   R108K

   Promoter

   Chicken Beta Actin

   Available Since

   Dec. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Zeo-mEGFP

   Plasmid

   #214145

   Purpose

   Expresses the monomeric EGFP in eukaryotic cells

   Depositor

   Bertrand Collet

   Article

   Chaumont et al J Immunol. 2024 Jul 26:ji2400076. doi: 1

   Insert

   mEGFP
   

   Expression

   Mammalian

   Mutation

   A207K L221K F223R

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-TRE-ChR2-YFP

   Plasmid

   #171622

   Purpose

   TRE-ChR2-YFP reporter

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   TRE-ChR2-YFP
   

   Use

   AAV and Synthetic Biology

   Expression

   Mammalian

   Promoter

   TetO

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-GalT

   Plasmid

   #11929

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Cole et al Science. 1996 Aug 9. 273(5276):797-801.

   Insert

   GalT (GALT Human)
   

   Expression

   Mammalian

   Mutation

   Amino acids 1-60 of the human galactosyltransfera…

   Available Since

   Sept. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV HA hRB-wt

   Plasmid

   #58905

   Purpose

   mammalian expression of wild type human Rb

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-HA-UBA6

   Plasmid

   #136995

   Purpose

   Expresses human UBA6 in mammalian cells

   Depositor

   Marcus Groettrup

   Article

   Pelzer et al J Biol Chem. 2007 Aug 10;282(32):23010-4

   Insert

   UBA6 (UBA6 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pC1-SypHer3s-dmito

   Plasmid

   #108119

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-dmito
   

   Tags

   ratiometric YFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-EF1alpha-SARS-CoV-2-N-2xStrep-IRES-Puro

    Plasmid

    #141391

    Purpose

    Lentiviral expression of SARS-CoV-2 nucleocapsid (N) protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-N (N SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available Since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. p6sheLLr

    Plasmid

    #37318

    Depositor

    John Schiller

    Article

    Pastrana et al Virology. 2005 Jul 5;337(2):365-72.

    Insert

    HPV6L1+L2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

    Plasmid

    #214995

    Purpose

    Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    EEA1 (EEA1 Human)
    

    Use

    Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Actin-C-18

    Plasmid

    #54967

    Purpose

    Localization: Actin, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Actin (ACTB Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. LSB-hsa-let-7a-5p

    Plasmid

    #103146

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-let-7a-5p target
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. Halo-Sec23A

    Plasmid

    #166892

    Purpose

    mammalian expression of Sec23A tagged with HaloTag

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    SEC23A (SEC23A Human)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. hPGK-rtTA3

    Plasmid

    #195883

    Purpose

    Lentiviral construct of hPGK driven rtTA3

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    rtTA3
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-HA-MyD88

    Plasmid

    #12287

    Depositor

    Bruce Beutler

    Article

    Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    MyD88 (MYD88 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. hIR-GFP

    Plasmid

    #22286

    Depositor

    Joseph Bass

    Article

    Ramos et al Proc Natl Acad Sci U S A. 2007 Jun 19. 1

    Insert

    INSR insulin receptor (INSR Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2 LRP6-eGFP

    Plasmid

    #180143

    Purpose

    to make movies of LRP6 vesicles

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDH-CMV-human MYCN-EF1-mRFP

    Plasmid

    #193708

    Purpose

    Lentiviral expression of human MYCN

    Depositor

    Martine Roussel

    Insert

    MYCN (MYCN Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge