78,128 results

1. pAAV-M13-TevC-IRES-SP6-Cre

   Plasmid

   #171620

   Purpose

   M13-TevC with Cre

   Depositor

   Hyungbae Kwon

   Article

   Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

   Insert

   M13-TevC-IRES-SP6-Cre
   

   Use

   AAV and Synthetic Biology

   Tags

   6x His

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Calreticulin-SuperNova-KDEL

   Plasmid

   #131857

   Purpose

   Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

   Depositor

   Geert van den Bogaart

   Article

   Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

   Insert

   SuperNova
   

   Tags

   KDEL ER-retention signal and Signal sequence of h…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLVX-EF1a-EGFP-TA Golgin84-IRES-Puromycin

   Plasmid

   #133028

   Purpose

   Expresses EGFP-tagged Golgi apparatus marker

   Depositor

   David Andrews

   Article

   Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

   Insert

   Golgin84 (GOLGA5 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   deleted amino acids 1-673

   Promoter

   Human elongation factor 1 alpha

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti CMV/TO Puro DEST (670-1)

   Plasmid

   #17293

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. LSB-hsa-let-7a-5p

   Plasmid

   #103146

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-let-7a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-let-7a-5p target
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   EF-1a

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. Halo-Sec23A

   Plasmid

   #166892

   Purpose

   mammalian expression of Sec23A tagged with HaloTag

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

   Insert

   SEC23A (SEC23A Human)
   

   Tags

   HaloTag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. hPGK-rtTA3

   Plasmid

   #195883

   Purpose

   Lentiviral construct of hPGK driven rtTA3

   Depositor

   Elaine Fuchs

   Article

   Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

   Insert

   rtTA3
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. mApple-LAMP1-phLuorin-N-8

   Plasmid

   #54918

   Purpose

   Localization: Lysosome Membrane, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   LAMP1 phLuorin
   

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Lysosomes-20 double labeled with phLuorin

   Promoter

   CMV

   Available Since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-SADB19P

   Plasmid

   #32631

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   SADB19P
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-pTH-iCre:EGFP-WPREpA

    Plasmid

    #213142

    Purpose

    Truncated rat TH promoter expressing iCre fused to EGFP

    Depositor

    Ulrik Gether

    Article

    Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

    Insert

    iCre
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    Truncated TH promoter from rat (Rattus norvegicus…

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. TALEN-CCR5L18-NtermN1

    Plasmid

    #51444

    Purpose

    Expresses TALEN targeting left CCR5A site (L18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Left (L18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 EL variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-Syn-ChrimsonR-GFP

    Plasmid

    #58806

    Purpose

    AAV expression of ChrimsonR-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    ChrimsonR-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K176R

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-PspCas13b-GEMSgRNA2

    Plasmid

    #204768

    Purpose

    pU6-PspCas13b-gRNA-Actb1216 backbone (Addgene ID: 155368) containing guide RNA #2 targeting the GEMS m6A reporter mRNA sequence.

    Depositor

    Kate Meyer

    Article

    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    guide RNA targeting GEMS m6A sensor mRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lmx1a-in situ probe

    Plasmid

    #45071

    Depositor

    Kathleen Millen

    Insert

    Lmx1a LIM homeobox transcription factor 1 alpha (residues 94-1149) (Lmx1a Mouse)
    

    Expression

    Mammalian

    Mutation

    Lmx1a residues 94-1149

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid

    #139996

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-hNurr1-NE

    Plasmid

    #102363

    Purpose

    Overexpression of NR4A2 nuclear receptor subfamily 4 group A member 2 in mammalian cells

    Depositor

    Shu Leong Ho

    Insert

    NR4A2 nuclear receptor subfamily 4 group A member 2 (NR4A2 Human)
    

    Tags

    NE

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK463 (BSD-P2A-mAB)

    Plasmid

    #214389

    Purpose

    mAID-BromoTag for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-mAID-BromoTag
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. TALEN-CCR5R18-NtermN1

    Plasmid

    #51445

    Purpose

    Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

    Depositor

    David Liu

    Article

    Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

    Insert

    TALEN CCR5 Right (R18) N1 N-term
    

    Use

    TALEN

    Tags

    3xFLAG and Fok1 KK variant

    Expression

    Mammalian

    Mutation

    K150Q

    Promoter

    CMV

    Available Since

    April 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-10nm-EBFP2

    Plasmid

    #231184

    Purpose

    Expresses a synthetic linker, tagged with EBFP2, Stabilizing the ER-mitochondrial distance at 10 nm

    Depositor

    Dmitry Lim

    Article

    Dematteis et al Commun Biol. 2024 Oct 10;7(1):1294. doi:

    Insert

    10nm-EBFP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGfaABC1D-nLac

    Plasmid

    #53131

    Purpose

    expression of lacZ driven by human GfaABC1D promoter

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2008 Apr;56(5):481-93. doi: 10.100

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains ABC1D regions of Gfa2 promoter, -1757 to…

    Promoter

    human Gfa2

    Available Since

    Oct. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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