75,815 results

1. pCMVtrunc msfGFPΔC10-beta-actin

   Plasmid

   #231548

   Purpose

   Mammalian expression of human beta actin fused to C-terminally truncated monomeric superfolder GFP, for actin filament organization measurements using fluorescence polarization microscopy

   Depositor

   Manos Mavrakis

   Article

   Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   msfGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMVtrunc

   Available since

   Jan. 30, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBiT3.1-C_WDR38:1-314

   Plasmid

   #211329

   Purpose

   Mammalian expression of full-length human WDR38. C-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   WDR38:M1-T314 (WDR38 Human)
   

   Use

   Tags

   GSSGGSSGVSGWRLFKKIS

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5'-3xFLAG minimal CMV pCDNA5

   Plasmid

   #212044

   Purpose

   LR destination vector for integration into N2a rtTA3 expressing cells with an FRT site in the Rosa26 locus

   Depositor

   Benjamin Blencowe

   Article

   Roth et al Mol Cell. 2023 Dec 7;83(23):4222-4238.e1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD649-HAsp-IGSF8-COMP5AP-AviTag-9xHis

   Plasmid

   #157476

   Purpose

   Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

   Depositor

   Chris Garcia

   Article

   Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

   Insert

   IGSF8 (IGSF8 Human)
   

   Use

   Tags

   COMP5AP-AviTag-9xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/SP6

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-UtrCH

   Plasmid

   #26740

   Depositor

   William Bement

   Article

   Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

   Insert

   Utrophin
   

   Use

   Reporter for f-actin

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Calponin homology domain of utrophin (aa# 1-261)

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP_CD47_SU

   Plasmid

   #65473

   Purpose

   GFP tagged CD47 with its own short 3'UTR

   Depositor

   Christine Mayr

   Article

   Berkovits et al Nature. 2015 Apr 20. doi: 10.1038/nature

   Inserts

   Signal peptide of CD47
   GFP
   CD47 coding region continued and short 3’UTR.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3

   Plasmid

   #172922

   Purpose

   For enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3

   Depositor

   GENIE Project

   Has Service

   AAV1 and AAV9

   Insert

   axon-jGCaMP8m-P2A-mRuby3
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain (N terminal on insert…

   Expression

   Mammalian

   Mutation

   Promoter

   hSynapsin1

   Available since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Str-KDEL_TNF-SBP-mCherry

   Plasmid

   #65279

   Purpose

   synchronize trafficking of TNF from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   TNF (TNF Human)
   

   Use

   Tags

   Streptavidin Binding Protein (SBP) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHCMV_SARS-CoV-2_Sdel18

   Plasmid

   #207307

   Purpose

   Mammalian expression of SARS2 coronavirus S protein

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   SARS-CoV2_S (S SARS2 coronavirus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mammalian codon optimization

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PCDNA3-GFP1-9 T2A mCherry

    Plasmid

    #124430

    Purpose

    Expresses GFP1-9 and T2A mCherry in mammalian cells

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    GFP1-9 T2A mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSFFV_sfCherry2_TagBFP

    Plasmid

    #83031

    Purpose

    Expresses full length sfCherry2 and TagBFP fusion in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry+full length_TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    3 mutations in sfCherry full length: E118Q, T128I…

    Promoter

    SFFV

    Available since

    Jan. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV500 A-FOS

    Plasmid

    #33353

    Depositor

    Charles Vinson

    Article

    Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

    Insert

    FOS (Fos Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative of c-Jun; heterodimerizes and i…

    Promoter

    CMV

    Available since

    Dec. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3 CB1

    Plasmid

    #13391

    Purpose

    Expresses mouse cannabinoid receptor 1 (CB1) in mammalian cells

    Depositor

    Mary Abood

    Article

    Abood et al Biochem Pharmacol. 1997 Jan 24. 53(2):20

    Insert

    CB1 (Cnr1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3-TopBP1-mCer

    Plasmid

    #211414

    Purpose

    mammalian expression of human TopBP1 C-terminally fused to mCerulean

    Depositor

    Helmut Pospiech

    Insert

    TopBP1 (TOPBP1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. L444P GBA pcDNA3.1

    Plasmid

    #188582

    Purpose

    Ectopic expression L444P GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L444P

    Promoter

    CMV

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CMVTO-TEVP

    Plasmid

    #179657

    Purpose

    Tobacco Etch Viral Protease

    Depositor

    Xiaojing Gao

    Article

    Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

    Insert

    TEVP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-CMV-puro_FlnaABD-TS-16flag

    Plasmid

    #234868

    Purpose

    Expresses control FlnA-based tension sensor that lacks dimerization domain and therefore does not report tension.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CMV-puro_FlnaABD-15-TS-16-24flag

    Plasmid

    #234869

    Purpose

    Expresses the FlnA actin binding domain and Ig15 , a FRET construct containing GFP-F40-RFP, the FlnA Ig 16 and Ig 24 domains.

    Depositor

    Michael Murrell

    Article

    Amiri et al Nat Commun. 2023 Dec 4;14(1):8011. doi:

    Insert

    FlnA Actin binding domain, Ig 15, GFP-F40-RFP, FlnaA Ig 16, FlnA Ig 24
    

    Use

    Lentiviral

    Tags

    GFP, RFP, FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AiP14573: pAAV-AiE0452h-minBG-FlpO-WPRE3-BGHpA (Alias: CN4573)

    Plasmid

    #230871

    Purpose

    AiE0452h is an enhancer sequence, designed to drive AAV-mediated transgene expression in striatal indirect pathway MSNs (D2-MSNs)

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Insert

    FlpO
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

    Plasmid

    #141383

    Purpose

    Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

    Depositor

    Nevan Krogan

    Article

    Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

    Insert

    SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)
    

    Use

    Lentiviral

    Tags

    2xStrep

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    EF1alpha

    Available since

    April 8, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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