78,128 results

1. pcDNA3.1-MS2-HA-HsSmg7_T

   Plasmid

   #147556

   Purpose

   Mammalian Expression of HsSmg7

   Depositor

   Elisa Izaurralde

   Article

   Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

   Insert

   HsSmg7 (SMG7 Human)
   

   Expression

   Mammalian

   Mutation

   two silent mutation G216A, A1521G and one non sil…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 7a sgRNA for EJ7-GFP and 4-μHOM reporters

   Plasmid

   #113620

   Purpose

   sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   7a sgRNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

3. pPRIME-CMV-LNGFR-FF3

   Plasmid

   #11666

   Purpose

   3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of LNGFR and miR30-based shRNA targeting firefly luciferase

   Depositor

   Stephen Elledge

   Article

   Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

   Insert

   FF3
   

   Use

   Lentiviral

   Tags

   LNGFR

   Expression

   Mammalian

   Available Since

   March 1, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-MS2-HA-HsStaufen2-T1_V

   Plasmid

   #147741

   Purpose

   Mammalian Expression of HsStaufen2-T1

   Depositor

   Elisa Izaurralde

   Insert

   HsStaufen2-T1 (STAU2 Human)
   

   Expression

   Mammalian

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAS

   Plasmid

   #206994

   Purpose

   Plasmid expressing both SpCas9 and SaCas9

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   SpCas9 - P2A - SaCas9 (NEWENTRY, NEWENTRY )
   

   Tags

   P2A

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDNR-gRNA

   Plasmid

   #206990

   Purpose

   A plasmid for expression of SaCas9-sgRNA targeting donor plasmids

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Insert

   Donor plasmid-targeting SaCas9-gRNA
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-IRES-puro-NLS-dPguCas13b-EGFP-NLS-3xFlag

   Plasmid

   #132400

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dPguCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   H151A; H1121A

   Available Since

   Jan. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. PABPC1

   Plasmid

   #155805

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   PABPC1 (PABPC1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgRNA-S1b (BsaI)-CBh-eCas12f1a

   Plasmid

   #233079

   Purpose

   Expression vector for sgRNA-S1b and eCas12f1a

   Depositor

   Kyoungmi Kim

   Article

   Park et al Nat Commun. 2025 Jan 15;16(1):677. doi:

   Insert

   U6-sgRNA-S1b (BsaI)-Cbh-bpNLS-eCas12f1a-2xFLAG-P2A-EGFP
   

   Use

   CRISPR

   Tags

   2xFLAG

   Expression

   Mammalian

   Promoter

   hU6, CBh

   Available Since

   April 25, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-DHB-mVenus-p2a-mCherry-CDK4KTR

    Plasmid

    #126679

    Purpose

    Fluorescent reporter for cyclin E/A-CDK and CDK4/6 activities

    Depositor

    Hee Won Yang

    Article

    Yang et al Elife. 2020 Apr 7;9. pii: 44571. doi: 10

    Insert

    Rb (a.a 886-928)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7_CC_Vpx-SIV_WPRE_IVT_Template

    Plasmid

    #216792

    Purpose

    Template for in vitro transcription of Vpx (SIV).

    Depositor

    Daniel Bauer

    Article

    Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

    Insert

    Vpx (SIV)
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4-NHK-MYC-His6

    Plasmid

    #185350

    Purpose

    Assessing of ubiquitination in cells

    Depositor

    Allan Weissman

    Article

    Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

    Insert

    a1-antitrypsin Hong Kong Null mutant
    

    Tags

    Myc, 6xHis

    Expression

    Mammalian

    Mutation

    NHK mutant

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-myc-Skp2

    Plasmid

    #19947

    Depositor

    Yue Xiong

    Article

    Ohta et al Cancer Res. 2001 Feb 15. 61(4):1347-53.

    Insert

    S-phase kinase-associated protein 2 (p45) (SKP2 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Available Since

    April 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-ZFN-AAVS1

    Plasmid

    #89707

    Purpose

    Mammalian expression plasmid for zinc finger nuclease targeting human AAVS1 locus

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Viruses. 2017 Nov 2;9(11). pii: v9110325

    Insert

    ZFN-AAVS1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-C1-mTrim21

    Plasmid

    #105516

    Purpose

    Expression of m-Cherry-tagged wildtype mouse Trim21 in mammalian cells

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCI-SEP GluR2(R)

    Plasmid

    #24001

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR2 (Gria2 Rat)
    

    Tags

    GluR1 signal peptide (aa1-22) and SEP (supereclip…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 5, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-AKAP79-AKAR4

    Plasmid

    #181957

    Purpose

    Genetically encoded FRET-based PKA activity reporter fused to AKAP79.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    AKAP79-AKAR4 (AKAP5 Human)
    

    Tags

    AKAP79, Cerulean, HIS tag, and cpVenus(E172)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3.1-V5-PTK2B

    Plasmid

    #127233

    Purpose

    Eukaryotic expression of PTK2B

    Depositor

    Kai Johnsson

    Article

    Haruki et al PLoS One. 2012;7(5):e37598. doi: 10.1371

    Insert

    PTK2B
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV500 A-C/EBPβ

    Plasmid

    #33363

    Depositor

    Charles Vinson

    Insert

    Cebpb (Cebpb Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Dominant Negative (see comments)

    Promoter

    CMV

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.2 TDP-43 NLS1, 5F-L YFP

    Plasmid

    #84913

    Purpose

    Mammalian expresion of TDP-43 NLS1, 5F-L YFP

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    K82A, R83A, K84A, F147L, F149L, F194L, F229L, F23…

    Promoter

    CMV

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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