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pSIQ

Plasmid

#216238

Purpose

All-in One IQ transgene switch, empty vector

Depositor

Hyunju Ro

Article

Hong et al Mol Ther Methods Clin Dev. 2024 Feb 2;32

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLgw EcoDam-V5-RFC1

Plasmid

#59209

Purpose

Mammalian DamID lentiviral vector for fusing gene of interest with Dam-V5 using Gateway cloning

Depositor

Bas van Steensel

Article

Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

Type

Empty backbone

Use

Lentiviral; Damid

Tags

Dam and V5

Expression

Mammalian

Promoter

Heat Shock Minimal Promoter

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-AID-BLM-UGI-T2A-mTagBFP2

Plasmid

#221222

Purpose

Express HACE Editor as a fusion of AID cytidine deaminase and BLM helicase with mTagBFP2 reporter

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

AID-BLM-UGI-T2A-mTagBFP2

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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5'UTR CGG 99x FMR1-EGFP

Plasmid

#63091

Purpose

Contains expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 as found in the Fragile X Tremor Ataxia Syndrome (FXTAS). These repeats are in frame with EGFP.

Depositor

Nicolas Charlet-Berguerand

Insert

expanded CGG repeats (99 repeats) within the 5'UTR of FMR1 (FMR1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1alpha-SARS-CoV-2-nsp8-2xStrep-IRES-Puro

Plasmid

#141374

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp8 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMK1254

Plasmid

#133057

Purpose

Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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HiBiT-FZD7

Plasmid

#195845

Purpose

Expresses human FZD7 with an N-terminal HiBiT

Depositor

Gunnar Schulte

Article

Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

Insert

FZD7 (FZD7 Human)

Tags

HiBiT

Expression

Mammalian

Promoter

CMV

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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Human SHIP1 (clone hp51CN)

Plasmid

#123347

Purpose

Expression of human SHIP1

Depositor

Christophe Erneux

Article

Drayer et al Biochem Biophys Res Commun. 1996 Aug 5;2

Insert

INPP5D (INPP5D Human)

Expression

Mammalian

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLJC6-2XFLAG-TMEM192

Plasmid

#104435

Purpose

Expresses 2XFLAG-TMEM192 in mammalian cells

Depositor

David Sabatini

Article

Wyant et al Cell. 2017 Oct 19;171(3):642-654.e12. do

Insert

TMEM-192 (TMEM192 Human)

Use

Lentiviral

Tags

2XFLAG

Expression

Mammalian

Promoter

Ubiquitin

Available Since

July 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO mTRPM7

Plasmid

#45482

Depositor

Andrew Scharenberg

Article

Nadler et al Nature. 2001 May 31;411(6837):590-5.

Insert

TRPM7 (Trpm7 Mouse)

Use

Tetracycline inducible

Tags

Flag

Expression

Mammalian

Promoter

Tetracycline-controlled CMV

Available Since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA6 MCV LTco

Plasmid

#40200

Depositor

Patrick Moore

Article

Shuda et al J Clin Invest. 2011 Sep;121(9):3623-34.

Insert

MCPyV LT codon optimized

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Ataxin3Q28

Plasmid

#22122

Depositor

Henry Paulson

Article

Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

Insert

Ataxin 3 (ATXN3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Oct. 1, 2009

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-hCA4-IV77

Plasmid

#244906

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-IV77

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pZFN-AAVS1_ELD

Plasmid

#159297

Purpose

ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI

Depositor

Kosuke Yusa

Insert

ZFN targeting the AAVS1 locus

Expression

Mammalian

Promoter

CMV promoter

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-IRES-GFP

Plasmid

#35396

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
GFP

Use

Retroviral

Expression

Mammalian

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
HaloKu70 sgRNA

Plasmid

#207583

Purpose

sgRNA for the insert of the HaloTag at the endogenous loci of Ku70.

Depositor

Jens Schmidt

Article

Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

Insert

GAGCAGTAGCCAACATGTCA

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCI-Puro_hCOL4A4-Myc-His

Plasmid

#198093

Purpose

Express hCOL4A4 in mammalian cells

Depositor

Jeffrey Miner

Insert

COL4A4 (COL4A4 Human)

Tags

TEV-Myc-His

Expression

Mammalian

Promoter

CMV, beta-globin/IgG chimeric intron

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP18

Plasmid

#22572

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP18 (USP18 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-Dest-pA-pgk-bsd

Plasmid

#74918

Purpose

Gateway destination piggyBac vector

Depositor

Jose Silva

Article

dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

Type

Empty backbone

Expression

Mammalian

Available Since

May 26, 2016

Availability

Academic Institutions and Nonprofits only