78,128 results

1. pcDNA5/FRT/TO_H2B-HaloTag10-T2A-EGFP

   Plasmid

   #175521

   Purpose

   Nuclear expression of HaloTag10 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   H2B-HaloTag10
   

   Expression

   Mammalian

   Mutation

   HaloTag10 = HaloTag7-Q165W

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9 TadDE

   Plasmid

   #193837

   Purpose

   Express TadDE (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-DualEditor-Cas9D10A-2xUGI
   

   Expression

   Mammalian

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cherry-Fibrillarin-C1

   Plasmid

   #134539

   Purpose

   Expresses Cherry-Fibrillarin fusion protein

   Depositor

   Susan Janicki

   Article

   Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

   Insert

   Fibrillarin (FBL Human)
   

   Tags

   Cherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDONR221_SLC25A31

   Plasmid

   #131969

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC25A31 (SLC25A31 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3-cdk5GFP

   Plasmid

   #1346

   Depositor

   Li-Huei Tsai

   Insert

   CDK5 (CDK5 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. mCherry-PCNA-19-SV40NLS-4

   Plasmid

   #55117

   Purpose

   Localization: Nucleus, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   PCNA-19 SV40NLS (PCNA Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

7. DDX5

   Plasmid

   #155523

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   DDX5 (DDX5 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNANOG-Luc

   Plasmid

   #25900

   Depositor

   Ren-he Xu

   Article

   Xu et al Cell Stem Cell. 2008 Aug 7. 3(2):196-206

   Insert

   NANOG promoter (NANOG Human)
   

   Tags

   Luciferase

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLJM1 polylinker

   Plasmid

   #164143

   Purpose

   Lentiviral expression vector pLJM1 with extended polylinker

   Depositor

   Richard Possemato

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVITRO1-Trastuzumab-IgA1/κ

    Plasmid

    #61888

    Purpose

    Expresses HER2/neu receptor specific humanized IgA1/κ antibody isotype (Trastuzumab-IgA1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Alpha 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Expression

    Mammalian

    Promoter

    mEF1 Prom and rEF1 Prom

    Available Since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-G-Flamp1-mut

    Plasmid

    #188568

    Purpose

    control probe of G-Flamp1, insensitive to cAMP

    Depositor

    Jun Chu

    Article

    Wang et al Nat Commun. 2022 Sep 12;13(1):5363. doi:

    Insert

    G-Flamp1-mut
    

    Tags

    His tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEBB Flag GCN5

    Plasmid

    #74784

    Purpose

    express of GCN5 in mammalian cells

    Depositor

    Ezra Burstein

    Article

    Mao et al Genes Dev. 2009 Apr 1;23(7):849-61. doi:

    Insert

    GCN5 (KAT2A Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    EF-1alpha promoter

    Available Since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hCas9

    Plasmid

    #51142

    Purpose

    Expresses hCas9 under the CAG promoter for CRISPR

    Depositor

    Izuho Hatada

    Article

    Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

    Insert

    hCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

14. AAV-P(Cry1)-DIO-intron336-dLUC

    Plasmid

    #110056

    Purpose

    Cry1 transcription luciferase reporter

    Depositor

    Eric Erquan Zhang

    Article

    Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

    Insert

    Cry1 promoter and luciferase (Cry1 Mouse)
    

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Promoter

    Cry1

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLVX-HA-UBE3A-IRES-Puro

    Plasmid

    #107349

    Purpose

    Expresses HA tagged UBE3A in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3A (UBE3A Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 23, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pBluescriptSKII+ U6-sgRNA(F+E) empty

    Plasmid

    #74707

    Purpose

    Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion of a spacer sequence

    Depositor

    Eugene Yeo

    Article

    Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

    Insert

    U6 promoter driving sgRNA with Bbsi sites for insertion of spacer sequence
    

    Expression

    Mammalian

    Available Since

    July 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAC_N_IRF1

    Plasmid

    #167760

    Purpose

    Expresses IRF1 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    IRF1 (IRF1 Human)
    

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Available Since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. hE-cadherin/Δp120

    Plasmid

    #45770

    Depositor

    Barry Gumbiner

    Article

    Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

    Insert

    hE-cadherin/Δp120 (CDH1 Human)
    

    Expression

    Mammalian

    Mutation

    Amino acids 762-764 converted from EED to AAA to …

    Promoter

    CMV

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

19. Nrxn3b-Nav1.6-MKII-f-hLOV1-TEVcs(ENLYFQ/M)-tTA-VP16

    Plasmid

    #163031

    Purpose

    FLiCRE TF with fast kinetics for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    Nrxn3b-MKII-f-hLOV1-TEVcs-tTA
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-neo-mOVA

    Plasmid

    #25099

    Depositor

    Maria Castro

    Article

    Yang et al Proc Natl Acad Sci U S A. 2010 Mar 9. 10

    Insert

    membrane bound ovalbumin
    

    Expression

    Bacterial and Mammalian

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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