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GFP-FBXL11

Plasmid

#126543

Purpose

Expresses GFP-tagged FBXL11 in mammalian cells

Depositor

Michele Pagano

Publication

Rona et al Elife. 2018 Jul 9;7. pii: 38771. doi: 10

Insert

FBXL11 (KDM2A Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

LTR

Available since

July 3, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLX209-neo-WRN K577M

Plasmid

#125790

Purpose

constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN K577M (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

K577M (helicase-dead)

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLX209-neo-WRN E84A

Plasmid

#125789

Purpose

constitutive, ectopic expression of an exonuclease-dead mutant of human WRN (E84A)

Depositor

Francisca Vazquez

Publication

Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

Insert

myc-WRN E84A (WRN Human)

Tags

5x myc

Expression

Mammalian

Mutation

E84A (exonuclease-dead)

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMF1707

Plasmid

#125584

Purpose

Expresses cytosolic roGFP2 (c-roGFP2) in mammalian cells

Depositor

Marc Fransen

Publication

Ivashchenko et al Mol Biol Cell. 2011 May;22(9):1440-51. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

c-roGFP2

Expression

Mammalian

Promoter

CMV

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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SaABEmax

Plasmid

#119814

Purpose

A-to-G base editor

Depositor

David Liu

Publication

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ecTadA-ecTadA*(7.10)-nSaCas9

Expression

Mammalian

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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RFP-ATF4

Plasmid

#125237

Purpose

ATF4 expression vector

Depositor

Rozita Yarmand

Publication

Bagheri-Yarmand et al J Biol Chem. 2015 May 1;290(18):11749-61

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ATF4 (ATF4 Human)

Tags

RFP

Expression

Mammalian

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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SECURE BE3(R33A)-P2A-EGFP (pJUL1085)

Plasmid

#123614

Purpose

CAG promoter expression plasmid for rAPOBEC1(R33A)-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP (SECURE variant BE3-R33A).

Depositor

Keith Joung

Publication

Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

BE3(R33A)-P2A-EGFP

Expression

Mammalian

Mutation

R33A in rAPOBEC1, D10A in SpCas9

Promoter

CAG

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG PSAM4 5HT3 LC IRES GFP

Plasmid

#123066

Purpose

Chemogenetic activator expression plasmid

Depositor

Scott Sternson

Publication

Magnus et al Science. 2019 Mar 14. pii: science.aav52

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)

Expression

Mammalian

Mutation

L131G Q139L Y217F

Promoter

CAG

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 WT-Puro

Plasmid

#123319

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Publication

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Plasmid

#107311

Purpose

Encoding scFv-p65-HSF1 driven by EF1α promoter

Depositor

Hui Yang

Publication

Zhou et al Nat Neurosci. 2018 Jan 15. pii: 10.1038/

Insert

EF1α-scFv-p65-HSF1-T2A-EGFP-WPRE-PolyA

Use

Lentiviral

Expression

Mammalian

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHBS1389 IBB-GFP-mCherry3E

Plasmid

#118803

Purpose

Fluorescence-based TDP43-dependent splicing reporter

Depositor

Rajat Rohatgi

Publication

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

Synthetic

Expression

Mammalian

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pZ148-BhCas12b-sgRNA-scaffold

Plasmid

#122448

Purpose

Expresses sgRNA for BhCas12b in mammalian cells

Depositor

Feng Zhang

Publication

Strecker et al Nat Commun. 2019 Jan 22;10(1):212. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

U6-BhCas12b-sgRNA-scaffold, CMV-mCherry

Expression

Mammalian

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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Tom20-CIB-GFP

Plasmid

#117242

Purpose

Light activated recruitment to OMM in mammalian cells with Cry2/CIB optogenetic switch

Depositor

Robert Hughes

Publication

Godwin et al J Biol Chem. 2019 Nov 8;294(45):16918-16

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CIBN

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLEX-FHH-Empty Vector-IRES-Puro

Plasmid

#120568

Purpose

For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

Depositor

Paul Khavari

Publication

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Type

Empty backbone

Use

Lentiviral

Tags

Flag-HA-6xHis

Expression

Mammalian

Promoter

CMV

Available since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3 - hSRSF6

Plasmid

#120419

Purpose

overexpression of human SRSF6

Depositor

Gillian Bates, Andreas Neueder

Publication

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (SRSF6 Human)

Expression

Mammalian

Promoter

CMV immediate/early

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA-3-VEGFR3

Plasmid

#119230

Purpose

Plasmid encodes full-length human VEGFR-3 gene cloned in mammalian expression vector.

Depositor

Kari Alitalo

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

VEGFR3 (FLT4 Human)

Expression

Mammalian

Promoter

CMV

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pZ M2rtTA_CAGG TetON-Sox10

Plasmid

#115240

Purpose

RMCE donor vector for inducible expression of SOX10 constitutive expression of m2rtTA (generated from plasmid #112668)

Depositor

Catherine Verfaillie

Publication

Garcia-Leon et al Stem Cell Reports. 2018 Feb 13;10(2):655

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SOX10 (SOX10 Human)

Expression

Mammalian

Available since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HDAC2-H141A

Plasmid

#115346

Purpose

Expresses mouse HDAC2 cDNA with H141A point mutation

Depositor

Javier Gonzalez-Maeso

Publication

Kurita et al Nat Neurosci. 2012 Sep;15(9):1245-54. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Histone deacetylase 2 (Hdac2 Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

H141A

Promoter

CMV

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PTEN

Plasmid

#116866

Purpose

Recruitable PTEN dual-specificity phosphatase

Depositor

Gerry Hammond

Publication

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PTEN (PTEN Human)

Tags

FKBP12 and mCherry

Expression

Mammalian

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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M3-PAmcherry1

Plasmid

#114189

Purpose

studying clustering of M3 receptors by superresolution microscopy (PALM)

Depositor

Aleksandra Radenovic

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Muscarin acetylcholine receptor 3 (CHRM3 Human)

Tags

3xHA and PAmCherry

Expression

Mammalian

Promoter

SV40

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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