78,130 results

1. TRE-mCherry-p2A-Chrimson-TS

   Plasmid

   #158702

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   TRE-mcherry-p2A-Chrimson-TS
   

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC081: pAAV.CMV.mCherry

   Plasmid

   #205744

   Purpose

   AAV vector for expressing CMV-driven mCherry reporter gene.

   Depositor

   Guei-Sheung Liu

   Article

   Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

   Insert

   mCherry
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. PMP34-mCherry-EGFP-Tet-On

   Plasmid

   #109017

   Purpose

   Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

   Depositor

   Jacob Corn

   Article

   Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

   Insert

   PMP34 (SLC25A17 Human)
   

   Use

   Lentiviral

   Tags

   mCherry-eGFP

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Aug. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMito-TrxRFP1

   Plasmid

   #98997

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   TrxRFP1 and mitochondrial localization sequence
   

   Tags

   His-tag and Mitochondria localization sequence

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. hNedd4-2

   Plasmid

   #83433

   Purpose

   full length Nedd4L with His Xpress tag

   Depositor

   Christie Thomas

   Article

   Itani et al Am J Physiol Renal Physiol. 2005 Aug;289

   Insert

   human Nedd4L with C2 domain (NEDD4L Human)
   

   Tags

   His Tag (6x) and Xpress Epitope Tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. TRE-UBC-hCD24-T2A-GFP

   Plasmid

   #205456

   Purpose

   lentiviral plasmid for expression of human CD24

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   hCD24 (CD24 Human)
   

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEYFP-Plk1(499)

   Plasmid

   #39843

   Depositor

   Jonathon Pines

   Article

   Lindon et al J Cell Biol. 2004 Jan 19;164(2):233-41.

   Insert

   Plk1 (PLK1 Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   Oct. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC0040-LwaCas13a crRNA backbone

   Plasmid

   #103851

   Purpose

   Backbone for cloning guides that are compatible with LwaCas13a. Contains a 5' direct repeat. Clone using BbsI (BpiI). F overhang AAAC. R overhang AAAA

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-LwaCas13aDR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA4/HisMaxB-YAP1-S127A

   Plasmid

   #18988

   Depositor

   Marius Sudol

   Article

   Oka et al J Biol Chem. 2008 Oct 10;283(41):27534-2

   Insert

   Yes-kinase associated protein S127A mutant (YAP1 Human)
   

   Tags

   His

   Expression

   Mammalian

   Mutation

   Serine 127 changed to Alanine, mutated protein t…

   Available Since

   Sept. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pDEST40-MCU-V5-HIS

    Plasmid

    #31731

    Depositor

    Vamsi Mootha

    Article

    Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

    Insert

    MCU (MCU Human)
    

    Tags

    V5-HIS6X

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. Galphai1-mTurquoise2

    Plasmid

    #69620

    Purpose

    The heterotrimeric G-protein subunit, Gαi isoform 1, tagged with mTurquoise2

    Depositor

    Dorus Gadella

    Article

    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

    Insert

    Galphai1-mTurquoise2
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

12. dCas13d-BFP

    Plasmid

    #215720

    Purpose

    Catalytically inactive dCasRx with 2A-BFP

    Depositor

    Chaolin Zhang

    Article

    Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

    Insert

    dCasRx
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    May 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. xCas9(3.7)-BE4

    Plasmid

    #108381

    Purpose

    Mammalian expression vector for xCas9(3.7)-BE4

    Depositor

    David Liu

    Article

    Hu et al Nature. 2018 Feb 28. pii: nature26155. d

    Insert

    xCas9(3.7)-BE4
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Available Since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF1627

    Plasmid

    #143144

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    FOXJ3 (FOXJ3 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTwist-SARS-CoV-2 Spike-deltaC18 JN.1

    Plasmid

    #216526

    Purpose

    Encodes SARS-CoV-2 spike for JN.1 pseudovirus production

    Depositor

    Alejandro Balazs

    Article

    Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

    Insert

    SARS-CoV-2 Spike JN.1 (S Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Mammalian

    Mutation

    See Depositor Comments.

    Promoter

    CAG

    Available Since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. K-SPOTIT1.0

    Plasmid

    #171215

    Purpose

    Expresses a kappa-opioid receptor-based circular permuted green fluorescent protein sensor

    Depositor

    Wenjing Wang

    Article

    Kroning et al Angew Chem Int Ed Engl. 2021 Mar 4. doi:

    Insert

    K-SPOTIT1.0
    

    Use

    Lentiviral

    Tags

    FLAG tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC1-HyPer-3

    Plasmid

    #42131

    Depositor

    Vsevolod Belousov

    Article

    Bilan et al ACS Chem Biol. 2012 Dec 20.

    Insert

    HYPER 3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid

    #131461

    Purpose

    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

    Depositor

    Joshua Breunig

    Article

    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

    Insert

    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    

    Use

    Cre/Lox and Synthetic Biology; Mosaic analysis fo…

    Tags

    Trp53-V5, H3F3A-EGFP-AU1

    Expression

    Mammalian

    Mutation

    Pdgfra D842V

    Promoter

    none (4x polyA to mitigate episomal expression)

    Available Since

    July 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid

    #63726

    Purpose

    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

    Depositor

    Mark Kay, Joseph Wu

    Article

    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103

    Insert

    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    

    Tags

    IRES-dtomato

    Expression

    Mammalian

    Mutation

    codon-optimised

    Promoter

    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-PGK-Neo-PIP-NLS-mVenus

    Plasmid

    #118619

    Purpose

    Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-NLS-mVenus
    

    Use

    Lentiviral

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    hPGK

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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