78,133 results

1. pLVX-TetOne-Puro-mitoEcSTH-FLAG

   Plasmid

   #218629

   Purpose

   This plasmid contains human codon optimized sequence of mitoEcSTH which is a soluble transhydrogenase from E. coli that can be used to increase NADH/NAD+ ratio in mammalian cells.

   Depositor

   Valentin Cracan

   Article

   Pan et al Nat Chem Biol. 2023 Oct 26. doi: 10.1038

   Insert

   mitoEcSTH
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   FLAG and MTS

   Expression

   Mammalian

   Available Since

   May 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-KDEL_TNF-SBP-RpHLuorin2

   Plasmid

   #171722

   Purpose

   Synchronize trafficking of TNFa-RpHLuorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   TNF (TNF Human)
   

   Tags

   Streptavidin Binding Protein (SBP) and ratiometri…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRK-FLAG-UbxD8

   Plasmid

   #53777

   Purpose

   express FLAG-tagged human UbxD8 gene

   Depositor

   Yihong Ye

   Article

   Xu et al J Biol Chem. 2013 Jun 21;288(25):18068-7

   Insert

   UbxD8 (FAF2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAcGFP1-N1-Dsup-C

   Plasmid

   #90022

   Purpose

   To express GFP-tagged C-terminal region of Dsup in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Hashimoto et al Nat Commun. 2016 Sep 20;7:12808. doi: 10

   Insert

   Dsup
   

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   C-terminal region alone

   Promoter

   CMV

   Available Since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmRFP_a_tubulin_IRES_puro2b

   Plasmid

   #21041

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   alpha Tubulin (TUBA1B Human)
   

   Tags

   pmRFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 9, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMGS7 (AAVS1sgRNA)

   Plasmid

   #126582

   Purpose

   A CRISPR/Cas9 construct to target the AAVS1 locus in humans

   Depositor

   Michael Guertin

   Article

   Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

   Insert

   AAVS1 sgRNA
   

   Expression

   Mammalian

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR221-SLCO2B1_STOP

   Plasmid

   #161032

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLCO2B1 (SLCO2B1 Human)
   

   Expression

   Mammalian

   Available Since

   June 21, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. PKC alpha CAT

   Plasmid

   #21234

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC alpha (PRKCA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta 1-325

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF2003

   Plasmid

   #142902

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   FOXD3 (FOXD3 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. EGFP-PIK3C2G

    Plasmid

    #171946

    Purpose

    Expresses PIK3C2G in mammalian cells

    Depositor

    Cheng-han Yu

    Article

    Feng et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    PIK3C2G (PIK3C2G Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-cox8-iPASnFR-L-mScarlet3-3xFLAG

    Plasmid

    #225240

    Purpose

    Mammalian expression of iPASnFR-L in mitochondria

    Depositor

    Loren Looger

    Article

    Tamura et al bioRxiv 2024.08.21.609037

    Insert

    iPASnFR-L
    

    Tags

    3xFLAG and mScarlet3

    Expression

    Mammalian

    Available Since

    Sept. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5/FRT/TO_LAMP1-HaloTag11-T2A-Tomn20-HaloTag9

    Plasmid

    #175523

    Purpose

    Co-expression of HaloTag11 (lyzosome) and 9 (mitochondrial surface) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    LAMP1-HaloTag11 and Tomm20-HaloTag9
    

    Expression

    Mammalian

    Mutation

    HaloTag11 = HaloTag7-M175W and HaloTag9 = HaloTag…

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHAGE-TO-nmdCas9-3XmCherry

    Plasmid

    #64110

    Purpose

    Expression of Nm dCas9-3XmCherry in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Nm dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XmCherry

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIG-V5-Snail2-IRES-nls-GFP

    Plasmid

    #44282

    Depositor

    Martin Cheung

    Article

    Liu et al Proc Natl Acad Sci U S A. 2013 Feb 19;11

    Insert

    V5-Snail2 (SNAI2 Chicken)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CMV-IE-enhancer and chicken beta-actin promoter

    Available Since

    Aug. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEF-HA/Net1A

    Plasmid

    #28262

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1A (Net1 Mouse)
    

    Tags

    2x HA

    Expression

    Mammalian

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pMIR-21 Reporter

    Plasmid

    #71870

    Purpose

    Mammalian expression vector for the analysis of miR-21 activity

    Depositor

    Mien-Chie Hung

    Article

    Shen et al Nature. 2013 May 16;497(7449):383-7. doi

    Insert

    Reverse complementary sequence of miR-21
    

    Tags

    firefly luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. TFORF2324

    Plasmid

    #144010

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TAF1 (TAF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-synapsin-HaloCaMP1b-EGFP

    Plasmid

    #138328

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1b
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    March 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. mKO2-2A-mTurquoise2

    Plasmid

    #98838

    Purpose

    Produces equimolar levels of two fluorescent proteins. It can be used to measure practical brightness and as a negative control for FRET

    Depositor

    Dorus Gadella

    Article

    Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

    Insert

    mKO2-2A-mTurquoise2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAG-YTB

    Plasmid

    #26721

    Depositor

    Edward Callaway

    Article

    Marshel et al Neuron. 2010 Aug 26. 67(4):562-74.

    Insert

    venusYFP, TVA950, Rabies Glycoprotein (RABVgp4, CD320 Chicken, Rabies virus (strain SAD B19))
    

    Expression

    Mammalian

    Available Since

    Feb. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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