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Mammalian Expression

76,333 results

Showing: 201 - 240 of 76333 results

  1. pCMV-enCas12a-A3A-Y130F

    Plasmid
    #193652
    Purpose
    Expression of base editor enCas12a-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    dLb-enCas12a-hA3A-Y130F, EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    dLbCas12a-D156R/G532R/K538R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193652-plasmid-map-sequence-id-422135

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  2. pLEX307-CTSB-blast

    Plasmid
    #158464
    Purpose
    Constitutive lentiviral expression of human CTSB.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSB (CTSB Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158464-plasmid-map-sequence-id-308167

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  4. pRSV-PKImut-v2

    Plasmid
    #45067
    Purpose
    Expression of intactive PKI (PKA inhibitor) mutant
    Depositor
    Richard Maurer
    Insert
    cAMP-dependent protein kinase inhibitor alpha
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Arg 20 & 21 to Glycines
    Promoter
    RSV
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
    45067-plasmid-map-sequence-id-62502

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  5. pLenti-C-Myc-DDK-IRES-Puro LARP7

    Plasmid
    #200725
    Purpose
    Lentiviral expression of Flag-Tagged human LARP7
    Depositor
    Claudio Alarcón
    Insert
    LARP7 (LARP7 Human)
    Use
    Lentiviral
    Tags
    Myc-DDK from backbone plasmid
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    200725-plasmid-map-sequence-id-403779

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  16. pD649-HAsp-AGER-Fc(DAPA)-AviTag-6xHis

    Plasmid
    #156761
    Purpose
    Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.
    Depositor
    Chris Garcia
    Article
    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.
    Insert
    AGER (AGER Human)
    Use
    Tags
    Fc(DAPA)-AviTag-6xHis
    Expression
    Mammalian
    Mutation
    Fc region of human IgG contains D265A and P329A m…
    Promoter
    CMV/SP6
    Available since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    156761-plasmid-map-sequence-id-302631

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  17. pTS2355_Tier3(PB)-iRFP_PuroR

    Plasmid
    #169654
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven iRFP670 and PuroR expression
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169654-plasmid-map-sequence-id-365633

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  20. pAAV2/6 v1

    Plasmid
    #240485
    Purpose
    AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein
    Depositor
    Addgene Research Program, Meghan Rego
    Insert
    AAV2 Rep and AAV6 Cap
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 25, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    240485-plasmid-map-sequence-id-474484

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  22. pLKO.1 hygro

    Plasmid
    #24150
    Purpose
    3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. Uses hygromycin for selection.
    Depositor
    Bob Weinberg
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for shRNA
    Available since
    March 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24150-plasmid-map-sequence-id-376525

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  26. pJRH-1362 scFv entry plasmid

    Plasmid
    #201912
    Purpose
    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Insert
    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201912-plasmid-map-sequence-id-403308

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  28. CROPseq-Guide-Puro

    Plasmid
    #86708
    Purpose
    Makes CRISPR gRNAs detectable by single-cell RNA-seq. The gRNA is expressed as part of the puromycin resistance mRNA transcribed by RNA Pol II and in its functional form from the hU6 promoter.
    Depositor
    Christoph Bock
    Article
    Datlinger et al Nat Methods. 2017 Jan 18. doi: 10.1038/n
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    Feb. 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    86708-plasmid-map-sequence-id-317051

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  32. pcDNA3-EGFP

    Plasmid
    #13031
    Purpose
    Mammalian expression vector for cloning your gene fused to EGFP
    Depositor
    Doug Golenbock
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 3, 2006
    Availability
    Academic Institutions and Nonprofits only
    13031-plasmid-map-sequence-id-305137

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Showing: 201 - 240 of 76333 results