74,931 results

1. pCI-neo FlucDM EGFP

   Plasmid

   #90172

   Purpose

   EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation

   Depositor

   Franz-Ulrich Hartl

   Article

   Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do

   Insert

   FLuc EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R188Q, R261Q in FLuc

   Promoter

   Available since

   Aug. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. YE1-BE4max

   Plasmid

   #138155

   Purpose

   C-to-T base editor

   Depositor

   David Liu

   Article

   Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103

   Insert

   rAPOBEC1(YE1)-nSpCas9-UGI-UGI
   

   Use

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Within rAPOBEC1 W90Y + R126E; within Cas9 D10A

   Promoter

   CMV

   Available since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lenti-Cas9-gRNA-GFP

   Plasmid

   #124770

   Purpose

   3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

   Depositor

   Jason Sheltzer

   Article

   Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTRIPZ (M)-HT-Cbx2

   Plasmid

   #82510

   Purpose

   Expresses Halotag-Cbx2 fusion proteins in mammalian cells

   Depositor

   Xiaojun Ren

   Insert

   Chromobox Homolog 2 (CBX2 Human)
   

   Use

   Lentiviral

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   aggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaacc…

   Available since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINE GFP nanobody:Halo:His6

   Plasmid

   #111090

   Purpose

   Bacterial expression of a fusion protein consisting of a GFP nanobody (Dr. Brett Collins, Addgene plasmid # 49172), the Halo tag and a His6 tag.

   Depositor

   Lennart Wirthmueller

   Article

   Chen et al Front Plant Sci. 2018 Nov 1;9:1581. doi:

   Insert

   GFP Nanobody
   

   Use

   Tags

   Halo and His6

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7 promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. mAG-GAL3

   Plasmid

   #62734

   Purpose

   Mammalian vesicle leakage reporter

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   mAG-Galectin 3
   

   Use

   Lentiviral

   Tags

   mAzami-Green

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-Alpha

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV_Stargazin-mTurquoise2-iLID

   Plasmid

   #161001

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   Stargazin-mTurquoise2-iLID
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. HBx

   Plasmid

   #65463

   Purpose

   expresses HBx in mammalian cells

   Depositor

   Wang-Shick Ryu

   Article

   Cha et al Hepatology. 2004 Jun;39(6):1683-93.

   Insert

   HBV X protein
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA 3.1(Hygro) myc-hPolQ-Flag

   Plasmid

   #73132

   Purpose

   To express in mammalian cells human PolQ

   Depositor

   Agnel Sfeir

   Article

   Mateos-Gomez et al Nature. 2015 Feb 12;518(7538):254-7. doi

   Insert

   Polymerase theta (POLQ Human)
   

   Use

   Tags

   Flag and myc

   Expression

   Mammalian

   Mutation

   Promoter

   cmv promoter

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLVX-opto-hCaspase-1

    Plasmid

    #208790

    Purpose

    Inducibly expresses human Caspase1, fused to light-activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    Caspase1 (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    Cry2 mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-NLS-HA-TurboID

    Plasmid

    #215075

    Purpose

    Expresses NLS-HA-TurboID in mammalian cells from a lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    TurboID (birA )
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HLA_DRB1_04_01

    Plasmid

    #165013

    Purpose

    Expression of HLA-DRB1*04:01 cDNA

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    HLA-DRB1*04:01 (HLA-DRB1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiCas9-EGFP

    Plasmid

    #63592

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and EGFP from EFS promoter. 3rd generation lentiviral backbone.

    Depositor

    Phil Sharp, Feng Zhang

    Article

    Chen et al Cell. 2015 Mar 12;160(6):1246-60. doi: 1

    Inserts

    Cas9
    EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and P2A

    Expression

    Mammalian

    Mutation

    Promoter

    EFS-NS

    Available since

    April 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLVX TRE 3G BFP

    Plasmid

    #128070

    Purpose

    Lentiviral expression of Tet inducible TagBFP

    Depositor

    Oskar Laur

    Insert

    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    no

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.22-PGK1-HRE::dLUC

    Plasmid

    #128095

    Purpose

    Hypoxia response element real-time luciferase reporter

    Depositor

    Chi Van Dang

    Article

    Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

    Insert

    Hypoxia response element (x3) from PGK1 promoter (PGK1 Human)
    

    Use

    Luciferase

    Tags

    drives expression of destabilized luciferase (2CP…

    Expression

    Mammalian

    Mutation

    Promoter

    inserted PGK1 hypoxia response elements (HRE) (x3)

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFP5290 ss-FIREmate-HA- KDEL-IVS-IRES- ss-SBP-mApple- FIREtag-GPI

    Plasmid

    #216694

    Purpose

    Synchronize trafficking of GPI-AP from the ER (RUCH system)

    Depositor

    Franck Perez

    Article

    Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

    Inserts

    FIREmate-KDEL
    SBP-mApple-FIREtag-GPI
    

    Use

    Tags

    FIREtag, IL2 signal sequence, Streptavidin Bindin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.2_mTagBFP2

    Plasmid

    #113725

    Purpose

    Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

    Depositor

    Vanessa LaPointe

    Article

    Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

    Insert

    mTagBFP2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG654 pFAST

    Plasmid

    #172861

    Purpose

    Expresses pFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

    Insert

    pFAST
    

    Use

    Tags

    Myc tag (C terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. plenti6-SLC7A11/xCT-V5

    Plasmid

    #170427

    Purpose

    Eepresses SLC7A11 (also named xCT) in mammalian cells

    Depositor

    Boyi Gan

    Article

    Koppula et al J Biol Chem. 2017 Aug 25;292(34):14240-1

    Insert

    solute carrier family 7 member 11 (SLC7A11 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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