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Plasmid#91205PurposeTransformation, Type: non-T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only
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pFH14
Plasmid#125799PurposeLevel0 SaCas9, Arabidopsis codon optimizedDepositorInsertSaCas9, Arabidopsis codon optimized
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHDE-35S-Cas9-mCherry
Plasmid#78931PurposeFor CRISPR/Cas9 mediated gene editing in Arabidopsis; provides a visual screen for Cas9-free plantsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNK869
Plasmid#219737PurposeMoClo-compatible Level 0 - promoter + 5U vector carrying promoter AtPD7DepositorInsertpromoter of serine carboxypeptidase-like gene AtSCPL30, AtPD7
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAtPD7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UnaG
Plasmid#203485PurposeFor recombinant protein production in Escherichia coli. His-tagged UnaG under the control of the T7 promoter.DepositorInsertUnaG
UseTagsExpressionBacterial and PlantMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_100
Plasmid#91198PurposeTransformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: noneDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GB4070
Plasmid#187811PurposeModule for the constitutive expression of Cup2:Gal4AD and gRNA-1 DFR, and the copper-inducible expression of dCasEV2.1 (dCas9:EDLL and MS2:VPR).DepositorInsertcopper-inducible dCasEV2.1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZA72
Plasmid#174783PurposeIn planta gene expressionDepositorInsertGG_XopJ4, Kmr plant selection marker
UseTagsExpressionPlantMutationWT + STOP codonPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPSup_iGFP
Plasmid#149420PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted just upstream of the minimal promoter. The GFP reporter gene harbors the IV2 intron.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC364
Plasmid#91213Purposeprotoplast vector expressing 2 gRNAs targeting tomato ARF8A (CmYLCV promoter, Csy4 processing)DepositorInsert2 gRNAs targeting tomato ARF8A
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AL13Rb
Plasmid#161006PurposePP7-tagged reporter without a promoterDepositorTypeEmpty backboneUseTagsPP7 and H2B-mScarletExpressionPlantMutationPromoterAvailable sinceDec. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135B2.0
Plasmid#167159PurposeGolden Gate entry vector to express the 5th gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAtU3Available sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pH-dCas9-PBE
Plasmid#98162PurposeExpresses CRISPR base editor in riceDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseRiceTagsExpressionPlantMutationPromoterUbiAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-mNeonGreen-GW
Plasmid#209352PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mNeonGreen fusion protein.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHdzCas9-KRAB
Plasmid#92341PurposeExpression dzCas9-KRAB in microalgae. CRISPR/Cas based plant genome editing and gene regulation, Hyg resistanceDepositorTypeEmpty backboneUseCRISPR; Plant expressionTagsKRABExpressionPlantMutationPromoterCaMV35SAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID-GFP
Plasmid#209403PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertPM-3XHA-TurbOID
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-NES-TurboID-GFP
Plasmid#209401PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a nuclear export signal (NES) in plantaDepositorInsertNES-3XHA-TurboID
UseTagsGFPExpressionPlantMutationTurboID gene sequence C249APromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGG-GFP-TurboID-3XNLS
Plasmid#209400PurposeTransiently expressing and visualizating the subcellular localization of TurboID fused with 3X nuclear localization signal (NLS) in plantaDepositorInsert3XHA-TurboID-3XNLS
UseTagsGFPExpressionPlantMutationPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable sinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pARSCL504
Plasmid#198636PurposeExpresses a transgene encoding RUBYDepositorInsert35S:Ω:Ruby
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pH-Ubi-miniSdd7-nCas9-PBE
Plasmid#204856PurposeFor cytosine base editing using miniSdd7 in monocotyledon plants (Agrobacterium-mediated transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1, OsU3, 35SAvailable sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYI001
Plasmid#170833Purpose(GUSPlus::tOCS in pFP100)*DepositorInsertGUSPlus::tOCS
UseTagsExpressionPlantMutationPromoterAvailable sinceOct. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAtC
Plasmid#78098PurposePlant expression of Cas9 and gRNA empty backbone; Hygro ResistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterU6Available sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECNUS4
Plasmid#184887PurposeABE cloning vector, insertion of gRNA sequence via Golden Gate with BsaI and T4 ligase, blue-white screening. Replicates in E.coli and Agrobacterium, binary vector for Agrobacterium T-DNA deliveryDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterRPS5AAvailable sinceJuly 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
P427-FBP_13
Plasmid#139704PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpSlH4:NnCPH:tSlH4, pSlRBCS2:AsNpga:tAtug7, pUBQ1:NhH3H:tNos, p35S:NnHisps:t35S, pAtELF4:NnLuz:trbcse9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH47802-p35S::tdTomato-ER::tNOS
Plasmid#160220PurposetdTomato fluorescent protein-ER localization, 35 promoter, tNOS terminator, transcriptional unit, inserted at the MoClo toolkit level 1 acceptor position 1 reverseDepositorInsertp2x35S::TdTomato-ER::tNOS
UseSynthetic Biology; Moclo toolkit level 1 acceptor…TagsExpressionPlantMutationPromoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2
Plasmid#96993PurposeExpress GFP-tagged mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationE2D (please see depositor comments below)Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTC394
Plasmid#91224Purposeprotoplast vector expressing gRNA24 targeting tomato ANT1 (control without TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGC1::YC3.60
Plasmid#107061PurposeExpressing Ca sensor yellow cameloen 3.6 under guard cell specific promoterDepositorInsertYC3.6
UseTagsExpressionPlantMutationPromoterGC1 (At1g22690)Available sinceApril 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPSint
Plasmid#149421PurposePlasmid for STARR-seq in tobacco leaves. Enhancer candidates can be inserted into the IV2 intron in the GFP reporter gene.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceJuly 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTC392
Plasmid#91223Purposeprotoplast vector expressing gRNA23 targeting tomato ANT1 (control without TREX2 expression)DepositorInsertgRNA targeting tomato ANT1
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGE666
Plasmid#153231PurposeRecipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAsDepositorTypeEmpty backboneUseCRISPRTagsGFPExpressionPlantMutationPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUB-spHRed
Plasmid#84734PurposeExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stomaDepositorInsertpHRed
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pB-dCas9-PBE
Plasmid#98159PurposeExpresses CRISPR base editor in maizeDepositorInsertNLS-APOBEC1-XTEN-dCas9-UGI-NLS
UseMaizeTagsExpressionPlantMutationPromoterUbiAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPPEM
Plasmid#162467PurposeDestination Expression vector for monocot plant primer editingDepositorInsertCas9H840A
UseTagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlaninePromotermaize UbiAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ146-ZmUbi-tRNA
Plasmid#158404PurposeGateway entry clone and Golden Gate recipient for pYPQ131-tRNA2.0 to pYPQ136-tRNA2.0; assembly of 6 gRNAsDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterMaize ubiquitin 1Available sinceJuly 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
UPG
Plasmid#161003Purposeubiquitous expression of PCP-GFPDepositorInsertPCP-GFP
UseTagsExpressionPlantMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35S:DsRed:Tnos (GB0361)
Plasmid#68220PurposeTranscriptional unit for DsRed plant expression driven by the 35S promoterDepositorInsertDsRed
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB412
Plasmid#74806PurposeGateway cloning compatible binary vector for N-terminal fusion with FLAG (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZmUBQ:GW
Plasmid#132358PurposeExpression of CDS from the Zea mays ubiquitin promotorDepositorTypeEmpty backboneUseTagsYFPExpressionPlantMutationPromoterpZmUBQAvailable sinceOct. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-miniSdd7-PBE
Plasmid#204859PurposeFor cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only