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P527

Plasmid

#139741

Purpose

Individual component of Caffeic Acid biosynthesis pathway, RcTAL

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

TAL

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pECNUS_MP01

Plasmid

#194350

Purpose

CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

Depositor

Eunyoung Chae

Article

Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

Insert

pcoCas9, FAST-Red

Use

Tags

Expression

Plant

Mutation

Promoter

RPS5A

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSW683 - pAHP6>>

Plasmid

#115998

Purpose

destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

Depositor

Sebastien Wolf

Article

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

pAHP6:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR

Use

Tags

HDEL and SP(ER)

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL86900OD

Plasmid

#86178

Purpose

GG compatible single transcript acceptor NPTII

Depositor

Mark Youles

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pYL1300UaUf

Plasmid

#183173

Purpose

Plant binary vector with ccdB flanked by Ua and Uf unique nucleotide sequences.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAGM65881

Plasmid

#153215

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pYPQ133A2.0

Plasmid

#99891

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pDL

Plasmid

#208978

Purpose

Dual-luciferase plasmid to measure enhancer activity in plants

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2024 Mar 21:koae088. doi: 10

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pFH17

Plasmid

#125803

Purpose

Level0 LbCas12a, rice codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, rice codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFLIPglu-600uDelta13

Plasmid

#12991

Depositor

Wolf Frommer

Article

Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

Insert

FLIPglu F16A Delta13

Use

Plant nanosensor

Tags

ECFP and EYFP

Expression

Plant

Mutation

F16A in binding protein

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only
pDGE651

Plasmid

#153229

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Gma-pCAMBIA-1301-STTM171

Plasmid

#84080

Purpose

Disturb the expression of miR171 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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PEP115E

Plasmid

#97401

Purpose

binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Use

Tags

Golgi target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAtCas9_3

Plasmid

#91158

Purpose

Cas9 only expression, Cas9 Type: AtCas9, Plant Selection: 2x35S:bar

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

AtCas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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LBJJ491

Plasmid

#117513

Purpose

AtYAO promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pYAO:AATG:BsaI

Use

Tags

Expression

Plant

Mutation

Promoter

AtYAO

Available since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMpGWB106-MTS-Citrine

Plasmid

#79135

Purpose

Binary vectors for expression of Citrine fluorescent protein with mitochondria localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

SD3(1-50)

Use

Tags

Citrine

Expression

Plant

Mutation

Promoter

CaMV 35S promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRGEB34

Plasmid

#158152

Purpose

Construction of inPTG-Cas9 plasmids with a truncated 5'-UTR intron for plant genome editing

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM81975

Plasmid

#219515

Purpose

Silencing of Nb RT

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Nb RT

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM10440

Plasmid

#219513

Purpose

Transient expression of At DFR

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At DFR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM54418

Plasmid

#219512

Purpose

Transient expression of Pz DFR

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

Pz DFR

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM10764

Plasmid

#219511

Purpose

Transient expression of At F3'H

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At F3'H

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGM10775

Plasmid

#219510

Purpose

Transient expression of At ANS

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Biotechnol J. 2023 Dec 20. doi: 10

Insert

At ANS

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

May 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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SPDK3182

Plasmid

#97404

Purpose

binary construct of ER-targeted sfYFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

ER-sfYFP1-10

Use

Tags

ER target

Expression

Plant

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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SPDK3184

Plasmid

#97405

Purpose

binary construct of cytosolic sfCFP1-10 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfCFP1-10

Use

Tags

cytosol

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pICSL50004

Plasmid

#50316

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, mCherry variant of RFP (Discosoma sp.)

Use

Tags

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Promoter

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pMCS-YFP-GW

Plasmid

#209361

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, with YFP tag

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pUBQ10-YFP-GW

Plasmid

#209362

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter driving expression of N-terminal YFP fusion protein.

Depositor

Lucia Strader

Article

Michniewicz et al BMC Res Notes. 2015 Mar 3;8:63. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCMU-MITr

Plasmid

#61198

Purpose

Fluorescent reporter for motochondria ScCOX4sp-mCherry expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

ScCOX4sp-mCherry

Use

Tags

mCherry

Expression

Plant

Mutation

Promoter

AtUBQ10

Available since

April 23, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL4723-P1

Plasmid

#191786

Purpose

Level P Golden gate accepter

Depositor

Wendy Harwood

Article

Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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