4,454 results
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Plasmid#138118PurposeGateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with D172R mutation, without promoterDepositorInsertMb2Cas12a v1
UseCRISPR; Gateway compatible cas12a entry cloneTagsNLSExpressionPlantMutationD172RAvailable SinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB216
Plasmid#68607PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsNuclear localization signal-tagged tdTomato (tdTo…ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDGB1a2R+pNOS_Red-F_NOSt
Plasmid#170888PurposeGoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Reverse orientationDepositorInsertRed-F
UseLuciferase and Synthetic BiologyExpressionPlantMutationp.S286Y Red mutantPromoterpNOSAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFGC5941-PacI-ath-STTM829.2
Plasmid#84158Purposetarget miRNA829.2 for destruction in Arabidopsis thalianaDepositorInsertSTTM829.2
ExpressionPlantPromoter2x35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV6:T35s
Plasmid#164741PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr3g095180.1
TagsTerminator 35SExpressionPlantPromoterLotus japonicus UbiquitinAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LjUBQ:MtGLV2:T35s
Plasmid#164740PurposeOverexpression of Peptide coding gene (Binary vector)DepositorInsertMedtr5g012400.1
TagsTerminator 35SExpressionPlantPromoterLotus japonicus UbiquitinAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSC2
Plasmid#91180PurposeT-DNA vector for combinatorial deletion of NCR genes in medicago truncatula (Csy4 array of 6 gRNAs under the control of CmYLCV promoter)DepositorInsertgRNAs targeting NCR genes
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TMEg
Plasmid#61209PurposeFluorescent reporter for plasma membrane and trunk peri-arbuscular membrane MtBCPsp-GFP-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-GFP-BCP
TagsEGFPExpressionPlantPromoterMtBCP1Available SinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TLEr
Plasmid#61188PurposeFluorescent reporter for transitory late endosome mCherry-MtRAB7A2 expressed under MtBCP1 promoterDepositorInsertMtRAB7A2
TagsmCherryExpressionPlantPromoterMtBCP1Available SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pZA57
Plasmid#174770PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-4xMyc, Kmr plant selection marker
Tags4xMycExpressionPlantMutationWTAvailable SinceDec. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ292 (AaCas12b)-D570A-TV
Plasmid#136380PurposeCRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation systemDepositorInsertAaCas12b (D570A)-TV
UseCRISPRExpressionPlantMutationD570AAvailable SinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB423
Plasmid#68688PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsTriple repeats of Citrine (3xCitrine)ExpressionPlantAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
MIGS4.2 (FF575)
Plasmid#35252DepositorTypeEmpty backboneExpressionPlantAvailable SinceMarch 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
P315-FBP_10
Plasmid#139701PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing CPH and NpgaDepositorInsertpUBQ1:NnH3H:tNos, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseExpressionPlantPromotervariousAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTX208
Plasmid#89268PurposeExpress STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01 and OsYSA-gRNA02DepositorInsertOsYSA-gRNA01 and OsYSA-gRNA02
UseCRISPRTagsSV40 NLSExpressionPlantPromoterMaize Ubiquitin1 promoterAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+pAtUbq10
Plasmid#170877PurposePhytobrick - pAtUBQ10DepositorInsertpAtUBQ10
ExpressionPlantMutation441C>T to remove BbsI restriction siteAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2+NtEUt
Plasmid#170883PurposePhytobrick - NtEUtDepositorInsertNtEUt
ExpressionPlantAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD+AtHSP18.2t
Plasmid#170884PurposePhytobrick - AtHSP18.2tDepositorInsertHSP18.2t
ExpressionPlantAvailable SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEELA
Plasmid#79752Purposebinary DEST vector for plant expressionDepositorTypeEmpty backboneExpressionPlantPromoter2xPro35SAvailable SinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-FIT2
Plasmid#96990PurposeExpress mouse FIT2 gene in plants.DepositorAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC32-SEIPIN3
Plasmid#96975PurposeExpress Arabidopsis SEIPIN3 gene in plants.DepositorAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB401+Citrine
Plasmid#128432PurposeModified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.DepositorTypeEmpty backboneUseGateway destination vectorTagsCitrine, yellow fluorescence proteinExpressionPlantAvailable SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHWBF06exp
Plasmid#130831PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertSUN2
TagsmCherry-FLAG-HAExpressionPlantPromoterCaMV 35SAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAtCas9-dead_1
Plasmid#91165PurposeCas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:hpt IIDepositorInsertAtCas9_D10A+H840A (dead)
UseCRISPRExpressionPlantMutationD10A, H840AAvailable SinceJuly 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEG302_dCAS9-MQ1(C141S,S317A)_g4+g10+g18
Plasmid#172317PurposeCRISPR dCas9 directly fused to a catalytic inactive version of bacterial DNA methyltransferase MQ1 to target to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(C141S,S317A)_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
TF101-STTM-miR165/166
Plasmid#84816PurposeTurning off/down miR165/166 in SoybeanDepositorInsertSTTM-miR165/166
ExpressionPlantPromoterunknownAvailable SinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0058
Plasmid#117539PurposeLevel1 Golden Gate Cassette: sgRNA cassette for SaCas9, targeting NbPDS gene in N. benthamianaDepositorInsertsgRNA (SaCas9)
ExpressionPlantAvailable SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN2
Plasmid#96977PurposeExpress GFP-tagged Arabidopsis SEIPIN2 gene in plants.DepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-SEIPIN1
Plasmid#96976PurposeExpress GFP-tagged Arabidopsis SEIPIN1 gene in plants.DepositorAvailable SinceJuly 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:ER-GFP1–10
Plasmid#125664PurposeBinary vector for the inducible expression of ER-targeted GFP1–10 fragment that can be used to detect a protein tagged with GFP11DepositorInsertER-GFP1–10
UseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceAug. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd SUP
Plasmid#115489PurposeCRISPR-Cas9 SunTag system to target NtDRMcd to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
35S-frGFP-nosT
Plasmid#80128PurposeTransient expression vector for frGFP in plantsDepositorTypeEmpty backboneTagsfrGFPExpressionPlantPromoterCaMV35SAvailable SinceDec. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJG493
Plasmid#91197PurposeWDV replicon T-DNA for gene targeting in wheat scutella, no Cas9 control (gUbi1+gUbi8+donor)DepositorInsertgUbi1+gUbi8+donor
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMU103
Plasmid#73934PurposeThis is hpRNA construct for gene silencing in soybean seeds. Soybean seed specific promoter, Glycinin gene promoter, is used.DepositorInsertbar and GUS cassettes
ExpressionPlantPromoter35SAvailable SinceJuly 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCO489
Plasmid#139732PurposeModule plasmid, individual component of Fungal Bioluminescent Pathway, LuzDepositorInsertpMOD_D_AtuMas:NnLuz:trbcSE9
UseLuciferaseExpressionPlantPromoterAtuMASAvailable SinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0040
Plasmid#117607PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_NbPDS(pEPOR1CB0058)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
tRNA-gRNA position [2_n-1] (GB1206)
Plasmid#75407PurposetRNA and scaffold for the assembly of GBoligomers for the intermediate position (positon [2_n-1]) of a polycistronic tRNA-gRNA (3-part multiplexing)DepositorInserttRNA-gRNA position [2_n-1]
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEG302 5aa SunTag VP64 g4+g17 (FWA)
Plasmid#119672PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with two guide RNAsDepositorInsertg17_U6_g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
ExpressionPlantPromoter2x35S+5'UTR OMEGA (pICH51288)Available SinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
PABE-2
Plasmid#115623PurposeTargeted A to G in wheat plants or monocotyledons protoplastsDepositorInsertwtTadA-TadA7.10-nCas9-NLS
UseCRISPRExpressionPlantAvailable SinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pZA71
Plasmid#174785PurposeIn planta gene expressionDepositorInsertGG_synthesized NbJIM2 (NbJIM2syn)-3xFLAG, Kmr plant selection marker
Tags3xFLAGExpressionPlantMutationWTAvailable SinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA68
Plasmid#174781PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-L17E/D481V-eGFP, Kmr plant selection marker
TagseGFPExpressionPlantMutationL17E/D481VAvailable SinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA66
Plasmid#174779PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-D481V-eGFP, Kmr plant selection marker
TagseGFPExpressionPlantMutationD481VAvailable SinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA65
Plasmid#174778PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-eGFP, Kmr plant selection marker
TagseGFPExpressionPlantMutationWTAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA67
Plasmid#174780PurposeIn planta gene expressionDepositorInsertGG_NbZAR1syn-L17E-eGFP, Kmr plant selection marker
TagseGFPExpressionPlantMutationL17EAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1 P35S:SV40-AcrIIA4_CONb:Tnos (GB3344)
Plasmid#160635PurposeTU for SV40-AcrIIA4 protein Codon optimized for N. benthamiana expression under the regulation of the 35S promoter and Tnos terminatorDepositorInsertAcrIIA4
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable SinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRATIO2251
Plasmid#141305PurposeGateway cloning compatible ratiometric binary vectorDepositorTypeEmpty backboneTagsFirefly Luciferase or LUC2 and mScarlet-IExpressionPlantPromoterUBQ10 promoterAvailable SinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB106-OEP7(1–50)
Plasmid#100602PurposeBinary vector for expression of Citrine-fused AtOEP7(1-50) (OEP7(1–50)-Citrine) to visualize chloroplast outer envelope membrane in plantsDepositorInsertOEP7(1–50)-Citrine (OEP7 Mustard Weed)
ExpressionPlantAvailable SinceFeb. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB221
Plasmid#68612PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagsModified EAR motif plant-specific repression doma…ExpressionPlantPromoterMpEF1alpha promoterAvailable SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB330
Plasmid#68658PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneTagstdTomatoExpressionPlantPromoterCauliflower mosaic virus 35S promoterAvailable SinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only