4,233 results
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Plasmid#197931PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the N-terminal and nucleoplasmin NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTagsFLAG tag and SV40 NLS and nucleoplasmin NLSExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBirA-P19
Plasmid#127919PurposeTransient expression of biotin ligase BirA and p19 in plant protoplastDepositorInsertBirA
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pnCas9-miniSdd7-PBE
Plasmid#204859PurposeFor cytosine base editing using miniSdd7 in monocotyledon plants (transient transformation)DepositorInsertminiSdd7-nSpCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A in SpCas9PromoterZmUbi-1Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRATIO4212
Plasmid#141313PurposeGateway cloning compatible dual fluorescent ratiometric binary vectorDepositorTypeEmpty backboneUseTagsmScarlet-I and mVenusExpressionPlantMutationPromoterUBQ10 promoterAvailable sinceSept. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDGE161
Plasmid#79453Purposerecipient plasmid [multiplexing]; pPcUbi:Cas9, FAST, nptIIDepositorInsertccdB (ccdB(letD) )
UseCRISPRTagsExpressionPlantMutationBsaI site eliminatedPromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPPEDs
Plasmid#162469Purposeprotoplast test vector of pPPEDDepositorInsertsCas9H840A
attR gateway destination cassette
UsePrime editing destination vector for transient ex…TagsMLV reverse transcriptaseExpressionPlantMutationChange histidine 840 to AlaninePromoterAvailable sinceDec. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
P305-FBP_4
Plasmid#139695PurposeT-DNA encoding Fungal Bioluminescent Pathway Components, complete pathwayDepositorInsertpLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
UseLuciferaseTagsExpressionPlantMutationPromotervariousAvailable sinceMay 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUTKan-3xHA-GFP-TOM5
Plasmid#130674PurposeUbiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial membrane of plantaDepositorInsertTranslocase of outer mitochondrial membrane 5 (TOM5 Mustard Weed)
UseTags3xHA and sGFPExpressionPlantMutationPromoterUbiquitin10Available sinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
PEP109E
Plasmid#97395Purposebinary construct of cytosolic mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMDC43-FIT2
Plasmid#96993PurposeExpress GFP-tagged mouse FIT2 gene in plants.DepositorInsertMmFIT2 (Fitm2 Mouse)
UseTagsGFPExpressionPlantMutationE2D (please see depositor comments below)Promoter35SAvailable sinceAug. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSC12
Plasmid#104786PurposepNB184-Cas9 entry cassetteDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB229
Plasmid#68620PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagstdTomatoExpressionPlantMutationPromoterAvailable sinceNov. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pUB-spHRed
Plasmid#84734PurposeExpresses pHRed in plant cells-biosensor for pHRed targeted to chloroplasts stomaDepositorInsertpHRed
UseTagsRbcS targeting sequenceExpressionPlantMutationPromoterubiqutin- 10 gene promoter (pUBQ10) of ArabidopsisAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35S
Plasmid#211839PurposePlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.DepositorInsertHR1b
UseSynthetic BiologyTagsGFPExpressionPlantMutationR181A R185APromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationPromoterUBIQUITIN10Available sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK-N. tabacum trnI/trnA
Plasmid#123579PurposeMoChlo kitDepositorInsertN. tabacum trnI/trnA integration site
UseChloroplast transformation vectorTagsExpressionPlantMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMB-TMEr
Plasmid#61208PurposeFluorescent reporter for plasma membrane and peri-arbuscular membrane MtBCPsp-mCherry-BCP expressed under MtBCP1 promoterDepositorInsertMtBCPsp-mCherry-BCP
UseTagsmCherryExpressionPlantMutationPromoterMtBCP1Available sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pOT2-poly-cis
Plasmid#141177PurposeTo clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoterDepositorTypeEmpty backboneUseRNAi and Synthetic BiologyTagsExpressionPlantMutationPromoterCaMV 35S promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265E6
Plasmid#213469PurposeGateway entry clone (attL1 & attR5) TadA-CDa-32aa linker-zSpCas9-D10A-2xUGI for C-T base editingDepositorInsertTadA-CDa-32aa linker-zSpCas9-D10A-2xUGI
UseCRISPR; Gateway compatible tada-cda-32aa linker-z…TagsNoExpressionPlantMutationPromoterAvailable sinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
dpYPQ230-D156R-ABE4
Plasmid#195370PurposedLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GGGGS)x6DepositorInsertecTadA8e-(GGGGS)x6-dLbCas12a-D156R
UseCRISPR; Gateway compatible ectada8e-(ggggs)x6-dlb…Tags5' and 3' NLSExpressionPlantMutationPromoterZmUBI1Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only