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MIGS3.1 (FF537)

Plasmid

#35249

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Promoter

CaMV 35S

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pDest_pK7WG2_pRPS5A_CTP OleGFP

Plasmid

#171723

Purpose

ptpTALECD vector assembly, Destination vector;

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2

Expression

Plant

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pTaCas9-D10A_5

Plasmid

#91170

Purpose

Cas9 only expression, Cas9 Type: TaCas9_D10A (nickase), Plant Selection: PvUbi2:hpt II

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

TaCas9_D10A (nickase)

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEGB3_alpha2 35:PhyB:VP16 (GB2745)

Plasmid

#160630

Purpose

TU for the expression of chimeric construct comprising the N-terminal fragment of A. thaliana PhyB (amino acids 1-650) fused to the VP16 activation domain and a nuclear localization sequence. Part of a red light-controlled synthetic genetic switch

Depositor

Diego Orzaez

Insert

PhyBVP16

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI and BsmBI sites removed

Promoter

35S

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM395

Plasmid

#84121

Purpose

target miRNA395 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM395

Expression

Plant

Promoter

2x35S

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pJG488

Plasmid

#91196

Purpose

WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

nTaCas9_H840A+gUbi8+gUbi1+donor

Use

CRISPR

Expression

Plant

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pBRN168

Plasmid

#79668

Purpose

DsREDmonomer-based BiFC vector for transient expression of RN (1-168) in plants.

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

DsRED-monomer (1–168)/RN168

Expression

Plant

Promoter

CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

Plasmid

#192421

Purpose

To act as the 2-input state of the OR gate.

Depositor

Ryan Lister

Article

Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

Insert

Act2::FRT-loxP-OCS-FRT-loxP::Rluc

Use

Synthetic Biology

Tags

PEST

Expression

Plant

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBGC155-MXMT

Plasmid

#79818

Purpose

GFP(S65T)-based BiFC vector for plants (Positive control: MXMT)

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Insert

CaMXMT1

Use

Reporter plasmid

Tags

GC155/GFP (155–239)

Expression

Plant

Promoter

CaMV 35S promoter and CaMV35S

Available Since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pBGN155

Plasmid

#74992

Purpose

GFP(S65T)-based BiFC vector for transient expression of sGN (1-155) in plants

Depositor

Yutaka Kodama, Masamitsu Wada

Article

Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

Type

Empty backbone

Use

Reporter plasmid

Tags

GFP (1–155)/GN155

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB102-Citrine-NLS

Plasmid

#79136

Purpose

Binary vectors for expression of Citrine fluorescent protein with a nuclear localization signal in plants

Depositor

Yutaka Kodama

Article

Ogasawara et al Plant Cell Environ. 2013 Feb 20. doi: 10

Insert

Citrine-NLS

Tags

NLS (Nuclear Localization Signal)

Expression

Plant

Promoter

CaMV 35S promoter

Available Since

Aug. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ292-D570A

Plasmid

#129679

Purpose

Gateway entry clone for CRISPR-Cas12b systems

Depositor

Yiping Qi

Article

Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

Insert

AaCas12b

Use

CRISPR; Gateway compatible aacas12b entry clone

Expression

Plant

Mutation

D570A; AaCas12b is rice codon optimized

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM166

Plasmid

#84197

Purpose

Disturb the expression of miR166 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR166

Expression

Bacterial, Plant, and Yeast…

Promoter

2x35S

Available Since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pG20_Venus_Blp

Plasmid

#159704

Purpose

pGREEN-based cloning construct for expression in planta

Depositor

Hans-Henning Kunz

Article

Pratt et al MicroPubl Biol. 2020 Oct 7;2020. doi: 10

Type

Empty backbone

Tags

mVenus

Expression

Plant

Available Since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM773a/b

Plasmid

#84141

Purpose

target miRNA773a/b for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM773a/b

Expression

Plant

Promoter

2x35S

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFH69

Plasmid

#128189

Purpose

Level1 ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

ZmUBIp::ABE7.10 base changer (human codon optimized):NOSt

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL080

Plasmid

#137889

Purpose

iSTU-CRISPR/Cas9 with OsCDPK2 intron, NU structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL085

Plasmid

#137893

Purpose

iSTU-CRISPR/Cas9 with RcCat intron, tRNA structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL083

Plasmid

#137892

Purpose

iSTU-CRISPR/Cas9 with RcCat intron, NU structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB125

Plasmid

#68579

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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