1,779 results

1. pHygG2r

   Plasmid

   #200069

   Purpose

   hygR selection nls-mNG marked rapid RMCE integration vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

   Type

   Empty backbone

   Use

   Recombination mediated cassette exchange

   Expression

   Worm

   Available Since

   May 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNMSB84

   Plasmid

   #199309

   Purpose

   MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

   Depositor

   Michael Krieg

   Article

   Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

   Insert

   flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ChR2-HDRC::SL2::jRGECO1a::let-858 3'UTR
   

   Expression

   Worm

   Mutation

   mTagBFP2 from pJJR81, ChR2(H134R;D156C), C. elega…

   Promoter

   flp-18

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJW2171

   Plasmid

   #163095

   Purpose

   CRISPR repair template: add insertion site specific homology arms by PCR before insertion

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::mNeonGreen (dpi)::AID*::3xFLAG::10aa linker
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Silent mutations to remove piRNA sites

   Available Since

   Jan. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSF11-wElectra1

   Plasmid

   #184932

   Purpose

   Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   Electra1
   

   Expression

   Worm

   Promoter

   tag-168

   Available Since

   Aug. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJW1659

   Plasmid

   #154314

   Purpose

   NT Donor-cassette for SapTrap

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   30aa linker::AID*::3xFLAG for NT slot
   

   Use

   CRISPR

   Expression

   Worm

   Available Since

   Aug. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. BiP 2: C. elegans BiP (hsp-4)-GFP

   Plasmid

   #21896

   Depositor

   David Ron

   Article

   Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

   Insert

   BiP (hsp-4 Nematode)
   

   Tags

   GFP

   Expression

   Worm

   Mutation

   Fusion between the GFP and hsp-4 takes place at A…

   Available Since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDD284

   Plasmid

   #66825

   Purpose

   TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   TagRFP-T-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized TagRFP-T

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pSEM414 - mosTI unc-119 Peft-3 - gfp(nls) - tbb-2

   Plasmid

   #182357

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and ubiquitous nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Expression

   Worm

   Promoter

   Peft-3

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPD95.75-wVenus

   Plasmid

   #37464

   Depositor

   Hitoshi Sawa

   Article

   Yamamoto et al PLoS Genet. 2011 Oct;7(10):e1002308. Epu

   Type

   Empty backbone

   Expression

   Worm

   Promoter

   none

   Available Since

   Aug. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159845

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJW1821

    Plasmid

    #163092

    Purpose

    CRISPR repair template: add insertion site specific homology arms by PCR before insertion

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    mScarlet^SEC (Lox511I)^::3xMyc
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJJR82

    Plasmid

    #75027

    Purpose

    EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    EGFP^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized EGFP

    Expression

    Worm

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJJR83

    Plasmid

    #75028

    Purpose

    mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Article

    Ramalho et al Development. 2020 Jul 22;147(14):dev1880

    Insert

    mCherry^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized mCherry

    Expression

    Worm

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBN1

    Plasmid

    #86710

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Rodenas et al Mol Biol Cell. 2012 Mar;23(5):930-44. do

    Insert

    Plmn-1::mCherry::his-58::pie-1 3'UTR
    

    Expression

    Worm

    Available Since

    Feb. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJW1816

    Plasmid

    #154320

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^3xMyc
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

    Plasmid

    #168173

    Purpose

    Expression of BeCyclOp in BWMs of C. elegans

    Depositor

    Alexander Gottschalk

    Article

    Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

    Insert

    BeCyclOp (codon optimized for C. elegans)
    

    Expression

    Worm

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSEM237 - pEXP[Prpl-3 | histamine | rpl-3 UTR]

    Plasmid

    #159796

    Purpose

    Histamine based negative selection marker to paralyze animals with arrays

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

    Insert

    pEXP[Prpl-3 | histamine | rpl-3 UTR]
    

    Expression

    Worm

    Mutation

    Not applicable

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. TU#867 (Punc-119sid-1)

    Plasmid

    #25811

    Depositor

    Martin Chalfie

    Article

    Calixto et al Nat Methods. 2010 Jul;7(7):554-9. doi: 1

    Insert

    systemic RNA interference-deficient 1 (sid-1 Nematode)
    

    Expression

    Worm

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pCFJ1415

    Plasmid

    #84828

    Purpose

    Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    C. elegans codon optimized GFP
    

    Expression

    Bacterial and Worm

    Promoter

    Peft-3

    Available Since

    Dec. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDD315 (mTurquoise2^SEC^2xHA)

    Plasmid

    #73343

    Purpose

    mTurquoise2^SEC^2xHA vector with ccdB sites for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Zhang et al J Cell Biol. 2023 Sep 4;222(9):e20230210

    Insert

    mTurquoise2-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    2xHA and C. elegans codon-optimized mTurquoise2

    Expression

    Worm

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge