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pWXC077

Plasmid

#222960

Purpose

C. elegans codon-optimized mScarlet3 with 3 introns cloned into pPD95.75 backbone

Depositor

Oliver Hobert

Publication

Cao et al Genetics. 2024 Oct 7;228(2):iyae126. doi

Insert

mScarlet3

Expression

Worm

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMDJ20 - pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Plasmid

#159808

Purpose

Optimized enzyme for genome editing in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP [Psmu-1 | Cre Recombinase (PATCs) | smu-1 UTR]

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1586

Plasmid

#121057

Purpose

mKate2^SEC^AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

mKate2-T^SEC^AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, C. elegans codon optimized mKate2, and au…

Expression

Worm

Available since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pBN42

Plasmid

#86713

Purpose

C. elegans injection marker

Depositor

Peter Askjaer

Publication

Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

Insert

Pmyo-3::GFP

Expression

Worm

Available since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSR49

Plasmid

#69259

Purpose

phlh-8::tagBFP2

Depositor

Sander van den Heuvel

Publication

Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

Insert

TagBFP2

Use

Cre/Lox

Expression

Worm

Mutation

codon-optimzed index 1.0, TagBFP2 contains 2 sing…

Promoter

hlh-8

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pEft-3::Cerulean-Venus::Tomm-7

Plasmid

#68046

Purpose

Ubiquitously expressed mitochondrial Tomm-7 tagged wtih oxCerulean-oxVenus (dFP, or double fluorescent protein) for monitoring mitophagy.

Depositor

Dana Miller

Publication

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pEft-3::Cerulean-Venus::Tomm-7

Expression

Worm

Available since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2

Plasmid

#61260

Purpose

Orsay virus RNA2 genome segment expression plasmid for virus reverse genetics

Depositor

David Wang

Publication

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Expression

Worm

Promoter

heat shock promoter

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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lis-1::L4440

Plasmid

#11336

Depositor

Guy Caldwell

Insert

lis-1 (lis-1 Nematode)

Use

RNAi

Expression

Worm

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only
L4816

Plasmid

#1682

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, gfpN, IVSR, gfpY66W, IVSS, gf…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGLOW77

Plasmid

#177338

Purpose

C. elegans mNeonGreen co-injection marker

Depositor

Ryan Doonan

Publication

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

Pmyo-2::mNeonGreen

Tags

mNeonGreen

Expression

Worm

Promoter

myo-2

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Plasmid

#159850

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS256

Plasmid

#73715

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS262

Plasmid

#73718

Purpose

C. elegans Pan-neuronal 2xNLS-FLP-D5 expression vector

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Psnt-1::2xNLS-FLP-D5

Expression

Worm

Mutation

G5D

Promoter

Psnt-1

Available since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pDYH5

Plasmid

#52032

Purpose

Expression of GFP-tagged DAF-16 in C. elegans

Depositor

Meng-Qiu Dong

Publication

Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

Insert

DAF-16 (daf-16 Nematode)

Tags

GFP and HIS

Expression

Worm

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pdestMB14

Plasmid

#26415

Depositor

Marc Vidal

Publication

Dupuy et al Genome Res. 2004 Oct;14(10B):2169-75.

Insert

attR4-ccdB-attR2

Expression

Bacterial and Worm

Available since

April 23, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lag-2 promoter:GFP (pJK590)

Plasmid

#26814

Depositor

Judith Kimble

Publication

Gao et al Proc Natl Acad Sci U S A. 1995 Oct 10. 9

Insert

lag-2 promoter

Tags

GFP

Expression

Worm

Available since

Dec. 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pJH4.67

Plasmid

#26948

Depositor

Geraldine Seydoux

Publication

Gallo et al Dev Biol. 2008 Nov 1. 323(1):76-87.

Insert

gamma-tubulin (tbg-1 Nematode)

Tags

GFP

Expression

Worm

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

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TU#712

Plasmid

#15280

Depositor

Martin Chalfie

Publication

Zhang et al Cell. 2004 Oct 1. 119(1):137-44.

Insert

nzyfp

Expression

Worm

Mutation

S65G, V68L, S72A, and T203Y

Available since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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L3785

Plasmid

#1592

Depositor

Andrew Fire

Type

Empty backbone

Tags

myo3-2341, myo3Tsa, T7p, gfpN, IVSR, gfpS65C, IVS…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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