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Showing: 701 - 750 of 1735 results

  1. pJJF439_PU6_empty_sgRNAscaffold - sgRNA cloning backbone

    Plasmid
    #164266
    Purpose
    sgRNA cloning backbone for expression of sgRNA in C. elegans. Contains BbsI sites for insertion of spacer sequences. U6 promoter from W05B2.8 gene.
    Depositor
    Nikolaus Rajewsky
    Article
    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Worm
    Mutation
    Promoter
    U6 promoter from W05B2.8
    Available since
    April 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    164266-plasmid-map-sequence-id-325780

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  10. pCFJ2060 - [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)

    Plasmid
    #159873
    Purpose
    Three-fragment Gateway compatible protein tag for expression in C. elegans
    Depositor
    Christian Froekjaer-Jensen
    Article
    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:
    Insert
    [1-2] ENTR - Tag - Snap (intron(1), syntrons(3), no_atg, no_stop)
    Use
    Tags
    Expression
    Worm
    Mutation
    Not applicable
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    159873-plasmid-map-sequence-id-319100

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  15. pJW1592

    Plasmid
    #121059
    Purpose
    GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.
    Depositor
    Jordan Ward
    Article
    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045
    Insert
    GFP^SEC^BioTag::AID*::3xFlag
    Use
    CRISPR and Cre/Lox
    Tags
    3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    121059-plasmid-map-sequence-id-235964

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  16. L4404

    Plasmid
    #1648
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    T7p, lacZN, OriF1>>, OriF1<<, T3p
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1648-plasmid-map-sequence-id-258

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  21. L4058

    Plasmid
    #1634
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    IVSA, pat3MbLS, gfpN, IVSR, gfpS65C, IVSS, IVST, …
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1634-plasmid-map-sequence-id-244

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  23. pMS74 (synthetic guide::∆HYGR::unc-54 3' UTR^SEC)

    Plasmid
    #154838
    Purpose
    Insertion of split hygromycin landing pad into ChrII:8420157 site C. elegans strains via CRISPR with SEC selection
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    synthetic guide::∆HYGR::unc-54 3' UTR
    Use
    CRISPR and Cre/Lox
    Tags
    Expression
    Worm
    Mutation
    ∆HYGR is promoterless and encodes aa 60-341
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154838-plasmid-map-sequence-id-307904

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  24. L3786

    Plasmid
    #1593
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    let858enh>>, let858enh<<, let858-455,…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1593-plasmid-map-sequence-id-203

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  25. pDD373

    Plasmid
    #137021
    Purpose
    SapTrap GFP-GLO donor
    Depositor
    Bob Goldstein, Daniel Dickinson
    Insert
    C. elegans GLO-optimized GFP
    Use
    CRISPR; Saptrap donor for cloning repair templat…
    Tags
    C. elegans GLO-optimized GFP
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    137021-plasmid-map-sequence-id-274046

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  26. L3787

    Plasmid
    #1594
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    hsp16-415', hsp16-25', T7p, gfpN, IVSR,…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1594-plasmid-map-sequence-id-204

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  36. pAS1-his-24::tagRFP

    Plasmid
    #124329
    Purpose
    Expressing Nuclear Localized tagRFP
    Depositor
    Andrew Leifer
    Insert
    tagRFP (his-24 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    124329-plasmid-map-sequence-id-241910

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  37. pPD8_33

    Plasmid
    #1468
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    SV40NLS, lacZN, lacZC
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1468-plasmid-map-sequence-id-79

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  38. L4663

    Plasmid
    #1665
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    Tags
    myo3-2341, myo3Tsa, SV40NLS, gfpN, IVSR, gfpY66W,…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1665-plasmid-map-sequence-id-275

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  41. L4400

    Plasmid
    #1647
    Depositor
    Andrew Fire
    Type
    Empty backbone
    Use
    RNAi
    Tags
    OriF1<<, OriF1>>, lacZN, T7p, unc2213…
    Expression
    Worm
    Mutation
    Promoter
    Available since
    March 7, 2005
    Availability
    Academic Institutions and Nonprofits only
    1647-plasmid-map-sequence-id-257

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  47. nud-1::L4440

    Plasmid
    #11337
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 12, 2006
    Availability
    Academic Institutions and Nonprofits only

  48. pCS2+ oDam_f_GFPoNLS

    Plasmid
    #85819
    Purpose
    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    oDam_f_GFPoNLS
    Use
    Tags
    oNLS (optimized nuclear localization signal) C te…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85819-plasmid-map-sequence-id-170478

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Showing: 701 - 750 of 1735 results