1,748 results

1. pJH3626

   Plasmid

   #179630

   Purpose

   Pacr-2(s)::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans A/B-MN-specific expression of miniSOG-SL2::RFP

   Depositor

   Mei Zhen

   Article

   Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

   Insert

   tomm20::miniSOG (tomm-20 Nematode)
   

   Use

   Tags

   RFP

   Expression

   Worm

   Mutation

   Promoter

   Pacr-2(s)

   Available since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCFJ2334 - [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)

   Plasmid

   #159850

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - ce-GFP(PATCs(900bp), NLS, no_atg, no stop)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSEM415 - mosTI unc-119 Pmex-5 - gfp(nls) - tbb-2

   Plasmid

   #182358

   Purpose

   Positive control for mosTI(unc-119). unc-119 rescue and germline nuclear GFP expression after mosTI insertion.

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Pmex-5

   Available since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pHW581

   Plasmid

   #198818

   Purpose

   In vivo calcium indicator. Presence of calcium (Ca2+) increases reporter signal intensity. Based on GCaMP7, improved SNR, fast kinetics

   Depositor

   Paul Sternberg

   Article

   Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

   Insert

   15xUAS::GCaMP7f-SL2-mKate2::let-858 3'UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGC185

   Plasmid

   #19624

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<4xNLS-tdimer2(12)::let-858(3’)
   

   Use

   Flp/frt

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAH34 (Phsp-16.41::cGAL-N::let-858 3'UTR)

   Plasmid

   #107133

   Purpose

   cGAL-N driver under the control of C. elegans hsp-16.41 promoter

   Depositor

   Paul Sternberg

   Article

   Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

   Insert

   NLS::cGAL(DBD)::gp41-1-N-intein
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   C. elegans hsp-16.41 promoter

   Available since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. XW09

   Plasmid

   #65833

   Purpose

   Punc-4c-QS-SL2-mcherry3'UTR-unc-54

   Depositor

   Kang Shen

   Article

   Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

   Insert

   Neurospora crassa OR74A repressor (qa-1S)
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   Promoter

   Punc-4c

   Available since

   Jan. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSN444

   Plasmid

   #108331

   Purpose

   Expresses Sat2 for Nourseothricin resistance selection of C. elegans

   Depositor

   Shinsuke Niwa

   Article

   Obinata et al PLoS One. 2018 May 9;13(5):e0197128. doi

   Insert

   Streptothricin acetyl transferase 2
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rps-27 promoter

   Available since

   Aug. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMS050

   Plasmid

   #91826

   Purpose

   Codon-optimized mScarlet-I donor for the SapTrap cloning system

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   mScarlet-I-C1
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMB70

    Plasmid

    #47943

    Purpose

    Can be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDD288

    Plasmid

    #137017

    Purpose

    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    mNG-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Pvha6-AgDD

    Plasmid

    #80626

    Purpose

    create protein aggregates in C. elegans

    Depositor

    Thomas Wandless

    Article

    Miyazaki et al Nat Commun. 2016 May 27;7:11689. doi: 10

    Insert

    NES-L106P-sfGFP (FKBP1A Human)
    

    Use

    Tags

    MLALKLAGLDI

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJA281

    Plasmid

    #22513

    Depositor

    Julie Ahringer

    Article

    Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

    Insert

    Pmex-5::mCherry (w introns w/o stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMDJ12 - pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]

    Plasmid

    #159799

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | mNeonGreen (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEY51

    Plasmid

    #191054

    Purpose

    mec-3 fluorescent neural reporter driving nuclear CyOFP1 and mTagBFP2 expression (refer to NeuroPAL paper for expression)

    Depositor

    Oliver Hobert, Eviatar Yemini

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    mec-3 (mec-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTK73.ChrII_ttTi5605.sgRNA

    Plasmid

    #194058

    Purpose

    sgRNA targeting ChrII_ttTi5605 locus of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ChrII_ttTi5605 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTK73.ChrV_oxTi365.gRNA

    Plasmid

    #194052

    Purpose

    sgRNA targeting ChrV_oxTi365 locus of the C. elegans genome

    Depositor

    Kota Mizumoto

    Article

    Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

    Insert

    ChrV_oxTi365 sgRNA
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Jan. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBN41

    Plasmid

    #86716

    Purpose

    C. elegans injection marker

    Depositor

    Peter Askjaer

    Article

    Morales-Martinez et al J Cell Sci. 2015 Mar 15;128(6):1090-6. d

    Insert

    Pmyo-2::GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Feb. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDSP15 (5’Δ HygR + loxP + MCS + 5’Δ mScarlet-I)

    Plasmid

    #193853

    Purpose

    Promoterless insertion vector for split HygR/mScarlet-I landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' rps-0::HygR + 5' mScarlet-I
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rps-0p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGLOW79

    Plasmid

    #177339

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-3::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-3

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge