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pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

Plasmid

#47550

Purpose

Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

Depositor

Bob Goldstein

Article

Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

Inserts

Cas9
ttTi5605 sgRNA

Use

CRISPR

Tags

HA and NLS

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Promoter

U6 and eef-1A.1 (eft-3)

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pSEZ-1

Plasmid

#51868

Purpose

Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

Depositor

Meng-Qiu Dong

Article

Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

Insert

psdhb-1::mtLS-cpYFP

Expression

Worm

Promoter

sdhb-1

Available Since

March 26, 2014

Availability

Academic Institutions and Nonprofits only

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pSEM371 - ce-unc-119 array integration

Plasmid

#182640

Purpose

Co-injection fragment for extra-chromosomal array integration at ce-unc-119 locus

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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coel::GFP

Plasmid

#8937

Depositor

Piali Sengupta

Article

Miyabayashi et al Dev Biol. 1999 Nov 15. 215(2):314-31.

Insert

unc-122 promoter (unc-122 Nematode)

Tags

GFP

Expression

Worm

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pJM483

Plasmid

#52987

Purpose

A secreted Lactadherin (sGFP::LactC1C2) Phosphatidylserine sensor

Depositor

Ding Xue

Article

Mapes et al Curr Biol. 2012 Jul 24;22(14):1267-75. d

Insert

Lactaherin (Mfge8 Mouse)

Tags

GFP (w/ introns) and signal sequence

Expression

Worm

Mutation

deleted native signal sequence, deleted C-termina…

Promoter

hsp-16.41

Available Since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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Ttx-3-mCherry-SL2-Post-SPHERE-SF-iGluSnFR

Plasmid

#187100

Purpose

Glutamate sensor for localization to membrane contatct

Depositor

Kotaro Oka

Article

Shindo et al bioRxiv 2022

Insert

Post-SPHERE-SF-iGluSnFR

Tags

mCherry-SL2

Expression

Worm

Promoter

Ttx-3

Available Since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHygG2

Plasmid

#200066

Purpose

hygR selection nls-mNG marked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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Pmec-18 caspase-3 (p12)::nz [TU#813]

Plasmid

#16082

Depositor

Martin Chalfie

Article

Chelur et al Proc Natl Acad Sci U S A. 2007 Feb 13. 1

Insert

caspase-3 (p12) :: nz (CASP3 Human)

Expression

Worm

Available Since

June 23, 2008

Availability

Academic Institutions and Nonprofits only

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TetR in pENTR1A (559-1)

Plasmid

#22265

Depositor

Eric Campeau

Insert

Tetracycline repressor (tetR )

Expression

Insect, Mammalian, Worm Exp…

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pJW1849

Plasmid

#154327

Purpose

sgRNA Target Vector for CRISPR

Depositor

Jordan Ward

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM320 - sgRNA mosTI unc-119 array insertion

Plasmid

#159824

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI unc-119 array insertion

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDACR3883 [rab-3p::HYlight-RA::let-858utr]

Plasmid

#207667

Purpose

Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

Depositor

Daniel Colón-Ramos

Article

Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

HYlight-RA [T152E]

Expression

Worm

Promoter

rab-3

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLJ57 (Pmec-17_GCaMP8f_SL2_mKate2_let-858_3'UTR)

Plasmid

#239865

Purpose

expression of GCaMP8f in C. elegans under mec-17-promoter control

Depositor

Monika Scholz

Article

Liu et al Genetics. 2024 Oct 7;228(2):iyae125. doi

Inserts

GCaMP8f
mKate2

Tags

6xHis

Expression

Worm

Mutation

codon optimized with introns for C. elegans

Promoter

mec-17

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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Punc-4c.TIR1

Plasmid

#194053

Purpose

TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

TIR1 (TIR1 Mustard Weed)

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

unc-4c

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDD287 (mKate2^SEC^3xMyc

Plasmid

#70685

Purpose

mKate2^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

mKate2-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mKate2

Expression

Worm

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCFJ151 - ttTi5605_MCS

Plasmid

#19330

Depositor

Erik Jorgensen

Article

Christian et al

Insert

ttTi5605 targeting region

Expression

Worm

Available Since

Oct. 29, 2008

Availability

Academic Institutions and Nonprofits only

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pCC324

Plasmid

#110165

Purpose

miniMos vector expresses ERKKTR-mClover and mCherry-H2B in C. elegans VPCs; request with plasmids pCC335 and pCC336 for unphosphorylated and phosphomimetic controls (Contains Neomycin resistance)

Depositor

Iva Greenwald

Article

de la Cova et al Dev Cell. 2017 Sep 11;42(5):542-553.e4.

Insert

ERK-KTR-mClover-T2A-mCherry-H2B

Use

Minimos transposon

Tags

mCherry and mClover

Expression

Worm

Mutation

R61K change made in ERK-KTR CDS

Promoter

lin-31 promoter/enhancer

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAS3-rab-3P::AI::ChR2(H134R)

Plasmid

#232601

Purpose

Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR

Depositor

Andrew Leifer

Article

Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

Insert

Channelrhodopsin2

Expression

Worm

Mutation

H134R

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHIP_RNA2FLAG

Plasmid

#61261

Purpose

Flag tagged Orsay virus RNA2 genome segment expression plasmid

Depositor

David Wang

Article

Jiang et al J Virol. 2014 Oct 15;88(20):11774-81. do

Insert

Orsay RNA2

Tags

Flag fused

Expression

Worm

Available Since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pWP017

Plasmid

#198780

Purpose

Drives specific expression in the ASI neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pgpa-4-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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