8,057 results

1. pAG425GPD-ccdB

   Plasmid

   #14154

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFA6a-link-ymScarletI3-SpHis5

   Plasmid

   #224076

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mScarletI3 with SpHis5 selection

   Depositor

   Bas Teusink

   Insert

   ymScarletI3
   

   Use

   Tags

   ymScarletI3

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFA6a-link-ymBaojin-SpHis5

   Plasmid

   #224082

   Purpose

   Yeast tagging vector to create a gene fusion with yeast optimized mBaojin with SpHis5 selection

   Depositor

   Bas Teusink

   Insert

   ymBaoJin
   

   Use

   Tags

   ymBaoJin

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCM184-ParB2::eGFP

   Plasmid

   #87252

   Purpose

   Expresses ParB2-EGFP in yeast. Also known as pOR2-GFP

   Depositor

   Kerstin Bystricky

   Article

   Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

   Insert

   ParB2
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   pBAD/tetO

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFA6a-mCherry-hphMX6

   Plasmid

   #105156

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Wang et al J Cell Biol. 2014 May 12;205(3):357-75.

   Insert

   mCherry-hphMX6
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pABY-c45

   Plasmid

   #201771

   Purpose

   Expresses mNeonGreen-tagged NbALFA under TEF1 promoter in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   NbALFA
   

   Use

   Tags

   40aa Linker, mNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   TEF1

   Available since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. bRA66

   Plasmid

   #100952

   Purpose

   GAL1 driven Cas9. gRNA can be cloned into BplI sites.

   Depositor

   James Haber

   Article

   Anand et al Nature. 2017 Apr 20;544(7650):377-380. d

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDEW8

   Plasmid

   #84238

   Purpose

   Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

   Depositor

   David Weinberg

   Article

   DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

   Insert

   HA-mRuby-P2A-eGFP
   

   Use

   Tags

   HA-mRuby and eGFP

   Expression

   Yeast

   Mutation

   Promoter

   GPD

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIP sHRPa-Aga1P

   Plasmid

   #73151

   Purpose

   Large fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.

   Depositor

   Alice Ting

   Article

   Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103

   Insert

   sHRPa-Aga1P
   

   Use

   Tags

   V5 tag

   Expression

   Yeast

   Mutation

   Promoter

   glyceraldehyde-3-phosphate dehydrogenase GPD prom…

   Available since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBHM2617

    Plasmid

    #234496

    Purpose

    NATR-CnU6-empty sgRNA

    Depositor

    Hiten Madhani

    Article

    Nalley et al bioRxiv 2025.02.14.638277

    Insert

    NAT-PCnU6-sgRNA_scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDRF1 mScarletI3-T2A-mTurquoise2

    Plasmid

    #219845

    Purpose

    equimolar expression of mScarletI3 and mTurquoise2 in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al bioRxiv 2025.06.27.661975; doi: https://

    Insert

    mScarletI3-T2A-mTurquoise2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. p415-GalL-Cas9-CYC1t

    Plasmid

    #43804

    Purpose

    A human codon-optimized Cas9 for expression in S. cerevisiae (budding yeast) from the GalL promoter

    Depositor

    George Church

    Article

    Dicarlo et al Nucleic Acids Res

    Insert

    Human Optimized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GalL

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCM189-ParB1::mCherry

    Plasmid

    #87253

    Purpose

    Expresses ParB1-mCherry in yeast. Also known as pOR1-mch

    Depositor

    Kerstin Bystricky

    Article

    Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

    Insert

    ParB1
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    pBAD/tetO

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRS420

    Plasmid

    #11259

    Depositor

    Fred Cross

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pABY-T2

    Plasmid

    #201765

    Purpose

    For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

    Depositor

    Saravanan Palani

    Article

    Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

    Insert

    ALFA tag
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HO-hisG-URA3-hisG-poly-HO

    Plasmid

    #51663

    Purpose

    yeast plasmid for integration of target sequence at HO locus, URA3 selection

    Depositor

    David Stillman

    Article

    Voth et al Nucleic Acids Res. 2001 Jun 15;29(12):E5

    Type

    Empty backbone

    Use

    Yeast integration

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAD-IRP

    Plasmid

    #220751

    Purpose

    Plasmid expresses the rabbit Iron Regulatory Protein fused to the Gal 4 Activation Domain.

    Depositor

    Marvin Wickens

    Article

    Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

    Insert

    Iron Regulatory Protein (ACO1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA6a-link-ytdTomato-URA3

    Plasmid

    #168054

    Purpose

    Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    ytdTomato

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP

    Plasmid

    #104394

    Purpose

    Yeast MCP-GFP expression

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    MCP-NLS-2xyeGFP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    MCP fused to NLS(SV40) and 2x yeast codon optimiz…

    Promoter

    CYC1

    Available since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ZJOM90

    Plasmid

    #133647

    Purpose

    Integration of SS-mCherry-HDEL in genome, use auxotrophic marker TRP1(Saccharomyces cerevisiae). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    pTPI1-SS-mCherry-HDEL
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge