8,265 results
-
Plasmid#41845DepositorInsert4-4-20
TagsHA and c-mycExpressionYeastAvailable SinceJan. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pV1382
Plasmid#111436PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_A08
Plasmid#84610PurposeIntroduces DSB at A08 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKL06
Plasmid#104430Purposesuper Ecliptic pHluorin with C terminal mRuby2 fusion tag in yeast expression vectorDepositorInsertSuper Ecliptic pHluorin-mRuby2
ExpressionYeastPromoterTef1Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDB5322
Plasmid#204832PurposeAn integrating plasmid containing both the inducible enotetSW4 promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetSW4 promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-ScAct1*-thymosinB-8His
Plasmid#111148PurposeExpresses Saccharomyces cerevisiae Act1 (codon is optimised for expression in Pichia pastoris) fused with thymosin beta and a His tag. TH3-37DepositorInsertAct1 (ACT1 Budding Yeast)
UsePichia pastoris integration plasmidTagsThymosin beta and a His-tagExpressionYeastPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAP05
Plasmid#102920PurposeDirect cloning and broad-host-range heterologous expression vectorDepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FRP2371
Plasmid#127577PurposeRepressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, His3MX selection markerDepositorInsertPRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1
UseSynthetic Biology; Shuttle vectorExpressionYeastAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMK425 (GAL-OsTIR1(F74A))
Plasmid#140657PurposeGAL-OsTIR1(F74A)DepositorInsertGAL-OsTIR1(F74A)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
p415TEF cyto roGFP2-Grx1(yeast)
Plasmid#65004PurposeYeast expression plasmid for roGFP2-Grx1; yeast codon optimized roGFP; yeast Grx1DepositorInsertGlutaredoxin-1
TagsroGFP2ExpressionYeastAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR_A08_hrGFP
Plasmid#84615PurposeHR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locusDepositorInserthrGFP
UseSynthetic BiologyExpressionYeastPromoterUAS1B8-TEF(136)Available SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pB_tetO7_sfGFP
Plasmid#196616PurposePlasmid for integrating tetO7-controlled sfGFP into the HO region in yeastDepositorInsertSuperfolder GFP
ExpressionYeastPromotertetO7 promoterAvailable SinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yEmCitrine-Hygro
Plasmid#44645DepositorInsertyEmCitrine
ExpressionYeastMutationA206RAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lact-C2-GFP-p416
Plasmid#22853DepositorInsertLactadherin-C2
TagsGFPExpressionYeastAvailable SinceFeb. 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAR-Ec611
Plasmid#130872PurposeA nuclear plasmid encoding an error-prone mutant TP-DNAP1 (I777K, L900S) for OrthoRepDepositorInsertTP-DNAP1
ExpressionYeastMutationI777K, L900SAvailable SinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pPFK300_Cas9
Plasmid#104910PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable SinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN4
Plasmid#180285PurposeKanR multi-gene yeast expression vector (GFP-dropout)DepositorInsertConLS' - GFP - ConRE' - KanR - CEN6/ARS4
ExpressionBacterial and YeastAvailable SinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZc-HsACTB*-thymosinB-8His
Plasmid#111146PurposeExpresses human beta-actin fused with thymosin beta and a His tag.DepositorInsertACTB (ACTB Human)
UsePichia pastoris integrationTagsThymosin beta and a His-tagExpressionYeastPromoterAOX1Available SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTU-DO-URA-C4
Plasmid#160339PurposeHigh-copy expression vector for K. marxianus, uracil selectionDepositorTypeEmpty backboneUseKluyveromyces marxianusExpressionYeastPromotern/aAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only