8,350 results
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Plasmid#188646PurposeExpresses Target-AID3S in yeastsDepositorInsertnCas9-AID2S-UGI
TagsUGIExpressionYeastMutationD10A for SpCas9PromoterpGal1Available SinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.028
Plasmid#184965PurposeTest effect of extending -Eco1 a1/a2 in yeastDepositorInsertEco1: RT and ncRNA(extended), a1/a2 length: 27
ExpressionYeastMutationhuman codon optimized RT, a1/a2 length extended t…PromoterGal7Available SinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
p415PGK1-AtMAX1(pYL759)
Plasmid#178289PurposeExpresses MAX1 from A. thaliana (AtMAX1) in Saccharomyces cerevisiae. Centromeric LEU, PPGK1-AtMAX1-Tpho5DepositorInsertAtMAX1
ExpressionYeastPromoterPGK1Available SinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYES2.1-cerMET16
Plasmid#107442PurposeOverexpression of MET fusion protein in S. cerevisiaeDepositorAvailable SinceApril 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKT0139
Plasmid#8731DepositorInsertyECitrine
TagsCodon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203YAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP37
Plasmid#65515PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-Cit-attR1 and attR2-Cer-cMycExpressionYeastPromoterpPMA1Available SinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pY3-LYS-IV
Plasmid#218119PurposeLYS-Leu2 plasmid (218114) with the functional cassettes of pY3 (218116) inserted at the sfGFP dropout locus.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
NM R(delta) 2-5 GFP
Plasmid#1209DepositorInsertsup35 NM domain (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutationlast four repeats of sup35 N domain deletedAvailable SinceMay 25, 2005AvailabilityAcademic Institutions and Nonprofits only -
YIp128-CUP1-NUbo-GFP-His6
Plasmid#212790PurposeCopper-inducible expression of the artificial test substrate NUbo-GFP-His6 in budding yeastDepositorInsertNUbo-GFP-His6
UseIntegrative vectorExpressionYeastPromoterCUP1Available SinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-natMX6-Purg1-3FLAG
Plasmid#19355DepositorInserturg1 promoter
ExpressionYeastAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pVG49
Plasmid#164707PurposeyEVenus under the control of GAL1 promoterDepositorInsertGAL1pr - yEVenus - CLN2 PEST - ADH1t
ExpressionYeastAvailable SinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDR-GW FLIPglu-30μΔ13V
Plasmid#27998DepositorInsertFLIPglu-30μΔ13V
TagseCFP and eYFPExpressionYeastMutationD154A in mglBAvailable SinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
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p416-GPD-PRUNE-HA
Plasmid#183945PurposeExpresses human HA-tagged PRUNE from yeast GPD promoterDepositorAvailable SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-ENT5-HaloTag
Plasmid#177102PurposeExpresses Ent5-HaloTag in yeast cellsDepositorInsertENT5 (ENT5 Budding Yeast)
ExpressionYeastAvailable SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETCON-TNF
Plasmid#67748PurposeTumor Necrosis Factor Alpha Yeast Display PlasmidDepositorInsertTNF-Alpha
ExpressionBacterial and YeastAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX-tetO-Pcyc1-GFP-LINKER
Plasmid#52687PurposeSame as 41025, with additional linkerDepositorInserttetO7-Pcyc1-GFP+LINKER
UsePromoter replacementExpressionYeastAvailable SinceMay 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG413 GAL ORF1 Shuffle1 NLS
Plasmid#145794PurposemPing Yeast Transposition AssayDepositorInsertORF1 Shuffle1 NLS
ExpressionYeastMutationEnhanced Nucleur Localization SignalAvailable SinceJan. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG425 GAL Pong TPase L418A, L420A
Plasmid#145795PurposemPing Yeast Transposition AssayDepositorInsertPong TPase L418A, L420A
ExpressionYeastMutationL418A, L420AAvailable SinceJan. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
p415 Glo3
Plasmid#112651PurposeExpression S.cerevisiaeDepositorInsertGlo3
ExpressionYeastPromoterMet25Available SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
plp
Plasmid#22310DepositorTypeEmpty backboneUseIntegrating plasmidExpressionYeastAvailable SinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomKO2-CaURA3
Plasmid#44881PurposeYeast tagging vector to create gene fusion with yeast optimized mKO2 with CaUra3 selectionDepositorInsertyomKO2
ExpressionYeastAvailable SinceMay 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCUP1pNuiHA kanMX CEN
Plasmid#131168Purposefor ScCUP1 promoter regulated N-terminal fused Nui-HA prey expression, centromeric ARS plasmid, kanMX marker confers resistance to geneticin in S. cerevisiaeDepositorTypeEmpty backboneTagsNui-HAExpressionYeastAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB4848
Plasmid#106138PurposeEasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistanceDepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS416-Aga1p-FAPB2.3.6L1TAG
Plasmid#158148PurposeYeast display reporter for non-display yeast strains (TAG construct)DepositorInsertAga1p-FAPB2.3.6
ExpressionYeastMutationCloned in a TAG codon at the first position of th…PromoterGAL1-10Available SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-TC-OM45-FKBPx4
Plasmid#104472PurposeIntegrating plasmid for overexpression of OM45-FKBPx4.DepositorAvailable SinceFeb. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK3F
Plasmid#85770PurposeN-ICE plasmid pK3FDepositorInsertloxP-KanMX-loxP-3FLAG
UseCre/LoxExpressionYeastAvailable SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXP216
Plasmid#26830DepositorTypeEmpty backboneUseCre/LoxExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAG414GAL-ccdB-HA
Plasmid#14239DepositorTypeEmpty backboneUseGateway CloningTags3HAExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
pXP322
Plasmid#26839DepositorTypeEmpty backboneExpressionYeastAvailable SinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
pRS403red-luxTA2-4F
Plasmid#166030PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS405red-phlTA2-1E
Plasmid#166029PurposeExpresses phlTA(2-1E) mutantDepositorInsertphlF
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationP5S, S6P, K86T, F109L, Q117R, E143K, codon-optimi…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGK401red-hENT1
Plasmid#166028PurposeExpresses human equilibrative nucleoside transporter 1 (hENT1)DepositorInserthuman equilibrative nucleoside transporter 1 (SLC29A1 Human)
UseSynthetic BiologyExpressionYeastPromoterpPGK1Available SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFTK036
Plasmid#171308PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertamdS ANIA_08777 acetamidase, selection marker
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDRFLIP36
Plasmid#65514PurposeFRET biosensor Destination vector for yeast expression. Encodes a FRET pair for fusion with ligand binding domain of interest.DepositorTypeEmpty backboneTags6His-AFPt9-attR1 and attR2-Cer-cMycExpressionYeastPromoterpPMA1Available SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPS811
Plasmid#8904DepositorAvailable SinceAug. 19, 2005AvailabilityAcademic Institutions and Nonprofits only -
pBS4
Plasmid#83793PurposeUseful for tagging yeast proteins with C-terminal CFP tag; hygromycin-B selectionDepositorInsertCFP
TagsCFPExpressionYeastAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
MmSphk2
Plasmid#118598PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in miceDepositorAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-Cerulean-GOS1
Plasmid#18846DepositorAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
YIplac211-Erg11-iGFPx3
Plasmid#115422PurposeExpress Erg11-iGFPx3 at the endogenous locus via pop-in pop-out mutagenesisDepositorInsertErg11-iGFPx3
TagsiGFPExpressionYeastPromoterEndogenousAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFTK087
Plasmid#171359PurposePart Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.DepositorInsertP-ANtRNA[Pro1]-sgRNA-dummy-Esp3I, Pol-III sgRNA-plug-in transcription unit
UseSynthetic BiologyExpressionBacterial and YeastMutationn/aAvailable SinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGK415-luxTA2-4F
Plasmid#165971PurposeExpresses luxTA(2-4F) mutantDepositorInsertluxR
UseSynthetic BiologyTagsNuclear localization signal and three copies of V…ExpressionYeastMutationS116Y, W201R, codon-optimized for expression in y…PromoterpPGK1 mutantAvailable SinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRPR1(1xTetO)_gRNA_handle_RPR1t
Plasmid#62966PurposeaTc inducible gRNA expression vectorDepositorTypeEmpty backboneExpressionYeastPromoterRPR1Available SinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECerulean-CaUra3
Plasmid#44648DepositorInsertyECerulean
ExpressionYeastAvailable SinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yomApple-SpHis5
Plasmid#44844PurposeYeast tagging vector to create gene fusion with yeast optimized mApple with SpHis5 selectionDepositorInsertyomApple
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMC-X4
Plasmid#176154Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XII1
Plasmid#176159Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI3
Plasmid#176157Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3DepositorTypeEmpty backboneExpressionYeastAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only