7,935 results

1. pRS316-GFP-COF1

   Plasmid

   #31843

   Depositor

   David Drubin

   Article

   Okreglak et al J Cell Biol. 2007 Sep 24;178(7):1251-64.

   Insert

   Cofilin (COF1 Budding Yeast)
   

   Use

   Tags

   GFP (S65T)

   Expression

   Yeast

   Mutation

   Promoter

   T7

   Available since

   Oct. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

2. pRG225

   Plasmid

   #64526

   Purpose

   Episomal E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pZHY500

   Plasmid

   #36186

   Depositor

   Daniel Voytas

   Article

   Zhang et al Plant Physiol. 2012 Nov 2.

   Insert

   FokI homodimeric Nuclease domain
   

   Use

   Tags

   ACV5

   Expression

   Yeast

   Mutation

   delta152,+63 truncation to TAL backbone

   Promoter

   TEV

   Available since

   Nov. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFA6a-hphMX6-P3nmt1-3FLAG

   Plasmid

   #19348

   Depositor

   Eishi Noguchi

   Article

   Noguchi et al Biotechnol J. 2008 Aug 26. ():.

   Insert

   hphMX6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCfB2224

   Plasmid

   #67547

   Purpose

   EasyClone system-based yeast integrative vector carrying loxP-flanked kanMX marker, integration into S. cerevisiae XI-2 chromosomal location, USER site for cloning, amp resistance

   Depositor

   Irina Borodina

   Article

   Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

   Type

   Empty backbone

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRS315e_pGal-PmCDA1

   Plasmid

   #79612

   Purpose

   Expresses PmCDA1 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   PmCDA1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   pGal1

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTEF-Sup35C

   Plasmid

   #1202

   Depositor

   Susan Lindquist

   Article

   True et al Nature 2004 Sep 9;431(7005):184-7. Epub

   Insert

   Sup35 C-terminus (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Sup35 C-terminus ~254aa-end

   Promoter

   TEF

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAH2

   Plasmid

   #41826

   Purpose

   Used as a PCR template for tagging yeast proteins with the DHFR tag by homologous recombination and selection for hygromycin resistance.

   Depositor

   Melissa Moore

   Article

   Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.

   Type

   Empty backbone

   Use

   Pcr template

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   T7, TEF

   Available since

   Oct. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAG306GAL-ccdB-EYFP

   Plasmid

   #14211

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EYFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFA-MNase-CaURA3

    Plasmid

    #162461

    Purpose

    DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-CaURA3

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pFA-MNase-CaHIS1

    Plasmid

    #162462

    Purpose

    DNA of the 3xFLAG epitope-MNase module for C. albicans. The PacI-AscI 3xFLAG-MNase fragment was cloned in the PacI-AscI-digested pFA-TAP-Ca HIS1

    Depositor

    Adnane Sellam

    Article

    Tebbji et al mSphere. 2020 Oct 14;5(5). pii: 5/5/e006

    Insert

    3FLAG-Mnase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. D588 lys2::HIS3 Disruptor Converter

    Plasmid

    #51675

    Purpose

    Yeast marker swap plasmid for converting LYS2 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGBW-m4046437

    Plasmid

    #145415

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pUDE722

    Plasmid

    #103022

    Purpose

    expression of a Cpf1 programming crRNA targetting CAN1 (crCAN1-4S)

    Depositor

    Jean-Marc Daran

    Article

    Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

    Insert

    crHCAN1-4.S (CAN1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    SNR52

    Available since

    Dec. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGBW-m4046431

    Plasmid

    #145526

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046476

    Plasmid

    #145512

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pPS2167

    Plasmid

    #1365

    Depositor

    Pamela Silver

    Article

    Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

    Insert

    RPL11B (RPL11B Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pTAL5-BB

    Plasmid

    #36033

    Depositor

    Tom Ellis

    Article

    Blount et al PLoS One. 2012;7(3):e33279. Epub 2012 Ma

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PGAL1

    Available since

    July 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HisSumoOphP

    Plasmid

    #189672

    Purpose

    HisSUMO tagged OphP to express OphP in pichia pastorias.

    Depositor

    James Naismith, Markus Künzler

    Article

    Emmanuel et al bioRxiv 2022.07.21.500988

    Insert

    HisSumoOphP
    

    Use

    Tags

    HisSumo tagged

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pXP831

    Plasmid

    #46065

    Depositor

    Nancy DaSilva

    Article

    Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CUP1

    Available since

    Sept. 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pCfB3041(gRNA X-3)

    Plasmid

    #73283

    Purpose

    EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site X-3

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Insert

    guiding RNA
    

    Use

    CRISPR; Grna

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCfB2192

    Plasmid

    #67539

    Purpose

    EasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae XII-2 chromosomal location, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Stovicek et al J Ind Microbiol Biotechnol. 2015 Sep 16.

    Type

    Empty backbone

    Use

    Cre/Lox; Integrative

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZEN

    Plasmid

    #129113

    Purpose

    Z. rouxii episomal vector containing ClonNAT resistance

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pZCAG

    Plasmid

    #129114

    Purpose

    Z. rouxii centromeric vector containing G418 resistance

    Depositor

    Paolo Giudici

    Article

    Bizzarri et al Yeast. 2019 Dec;36(12):711-722. doi: 10.

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAG305GAL-ccdB-HA

    Plasmid

    #14233

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    3HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGBDU-Atg17

    Plasmid

    #121394

    Purpose

    For Yeast-two-hybrid analysis

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    ATG17
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDN-T1TCbt

    Plasmid

    #44514

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDIV490

    Plasmid

    #177698

    Purpose

    Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

    Depositor

    Uffe Mortensen

    Article

    Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

    Inserts

    Cas9
    Nat
    

    Use

    CRISPR

    Tags

    SV40

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    RHR2p (Debaryomyces hansenii ) and TDH3p (Candida…

    Available since

    March 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pNXgate33-3HA

    Plasmid

    #105075

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloning

    Depositor

    Wolf Frommer

    Article

    Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

    Type

    Empty backbone

    Use

    Tags

    3xHA and NubG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRS315e_pGal-dCas9-SH3

    Plasmid

    #79613

    Purpose

    Expresses dCas9-SH3 in yeast cells

    Depositor

    Akihiko Kondo

    Article

    Nishida et al Science. 2016 Aug 4. pii: aaf8729.

    Insert

    SpCas9
    

    Use

    Tags

    SH3 domain

    Expression

    Yeast

    Mutation

    D10A and H840A for nuclease deficient Cas9

    Promoter

    pGal1

    Available since

    July 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDN-T1GZmbh

    Plasmid

    #44522

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-T123 promoter (GAL1 Budding Yeast, Synthetic)
    yEGFP::ZeoR
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Tags

    Expression

    Yeast

    Mutation

    Three tandem tet operators (3XtetO2) downstream o…

    Promoter

    PGal1-T123 promoter

    Available since

    April 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKT0174

    Plasmid

    #8720

    Depositor

    Kurt Thorn

    Article

    Sheff et al Yeast 2004 Jun;21(8):661-70.

    Insert

    yECFP
    

    Use

    Tags

    Codon optimized linker

    Expression

    Yeast

    Mutation

    yEGFP with F64L, S65T, Y66W, N144I, M153T, V163A

    Promoter

    Available since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGBW-m4046475

    Plasmid

    #145531

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pCS2+ oDam_f_GFPoNLS

    Plasmid

    #85819

    Purpose

    iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

    Depositor

    Joachim Wittbrodt

    Article

    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

    Insert

    oDam_f_GFPoNLS
    

    Use

    Tags

    oNLS (optimized nuclear localization signal) C te…

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    L122A. Codon optimization compatible to work in M…

    Promoter

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSK226

    Plasmid

    #129268

    Purpose

    dual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporter

    Depositor

    Ahmad Khalil

    Article

    Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

    Insert

    Rnq1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. Ube2d2

    Plasmid

    #31410

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2D2 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

37. GPD 25QDProGFP p416

    Plasmid

    #15568

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q Delta Pro (HTT Human)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

38. pCfB4586

    Plasmid

    #106124

    Purpose

    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked Ura3 marker, integration into Yarrowia lipolytica chromosomal location IntB, USER site for cloning, amp resistance

    Depositor

    Irina Borodina

    Article

    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. GAL FLAG46QDProCFPp303

    Plasmid

    #15585

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q Delta Pro (HTT Human)
    

    Use

    Tags

    CFP and FLAG

    Expression

    Yeast

    Mutation

    Proline-rich region removed

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

40. pSec7-Halox2

    Plasmid

    #115425

    Purpose

    Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec7-Halox2
    

    Use

    Tags

    HaloTag

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge