7,935 results

1. TM104b(WT)

   Plasmid

   #1159

   Depositor

   Susan Lindquist

   Article

   Schirmer et al Mol Biol Cell 2004 May;15(5):2061-72. Ep

   Insert

   HSP104 (HSP104 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

2. LPH2776 - Ub-I44A

   Plasmid

   #32163

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   I44A

   Promoter

   CUP1

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

3. pRS424-TUB2 - human TUBB6 Cterminal tail

   Plasmid

   #60403

   Purpose

   Expression of chimeric yeast beta tubulin (TUB2) - human beta5 tubulin Cterminal tail (TUBB6) using a GAL promoter

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   TUB2 amino acids 429 - end, replaced with human T…

   Promoter

   GAL

   Available since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pKT0240

   Plasmid

   #8739

   Depositor

   Kurt Thorn

   Article

   Sheff et al Yeast 2004 Jun;21(8):661-70.

   Insert

   yECitrine
   

   Use

   Tags

   13x Myc and Codon optimized linker

   Expression

   Yeast

   Mutation

   yEGFP with S65G, V68L, Q69M, S72A, T203Y

   Promoter

   Available since

   Feb. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNB788

   Plasmid

   #60165

   Purpose

   Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by RNR1 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   FLuc-yEVenus
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   FLuc has A4V & S504G (no functional effect)

   Promoter

   RNR1pr

   Available since

   March 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pNB691

   Plasmid

   #60135

   Purpose

   Red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.

   Depositor

   Nicolas Buchler

   Article

   Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

   Insert

   RdLuc
   

   Use

   Tags

   ClpXP degron and Nuclear localization signal (NLS…

   Expression

   Yeast

   Mutation

   Promoter

   MET17pr

   Available since

   Oct. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3x_a1gRNAOp_pCYC1m_yeBFP2

   Plasmid

   #64391

   Purpose

   encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

   Depositor

   Timothy Lu

   Article

   Farzadfard et al ACS Synth Biol. 2013 Sep 11.

   Insert

   yeast enhanced BFP2
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

8. sup35-R320I

   Plasmid

   #1199

   Depositor

   Susan Lindquist

   Article

   True et al Nature 2004 Sep 9;431(7005):184-7. Epub

   Insert

   Sup35p (SUP35 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed arg 320 to isoleucine

   Promoter

   Available since

   May 25, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pRG227

   Plasmid

   #64532

   Purpose

   Episomal E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAG413GPD-EYFP-ccdB

    Plasmid

    #14334

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    EYFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p404GALL

    Plasmid

    #17418

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    GALL promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GALL promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFA6a-link-yoMaxGFP-Kan

    Plasmid

    #44907

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized MaxGFP with Kan selection

    Depositor

    Kurt Thorn, Wendell Lim

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yoMaxGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pJJH1307

    Plasmid

    #36910

    Depositor

    Jürgen Heinisch

    Article

    Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

    Insert

    ScLEU2
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial, Mammalian, and Y…

    Mutation

    Promoter

    Available since

    July 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pNB753

    Plasmid

    #60148

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by LEU1 promoter.

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Yeast

    Mutation

    Promoter

    LEU1pr

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRPR1_c6gRNA_RPR1t

    Plasmid

    #64384

    Purpose

    encodes c6 gRNA

    Depositor

    Timothy Lu

    Article

    Farzadfard et al ACS Synth Biol. 2013 Sep 11.

    Insert

    c6 gRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 5, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pAG303GAL-ccdB-DsRed

    Plasmid

    #14349

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    DsRed

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAG306GAL-ccdB-Cerulean

    Plasmid

    #14379

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p41Neo 3-F GW

    Plasmid

    #58554

    Purpose

    CEN vector with G418 resistance marker for triple Gateway insertion

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LexA-Fus3

    Plasmid

    #18899

    Depositor

    Elaine Elion

    Article

    Elion et al Cell. 1990 Feb 23. 60(4):649-64.

    Insert

    FUS3 (FUS3 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 27, 2008

    Availability

    Academic Institutions and Nonprofits only

20. Gal4pBS24-Pleum

    Plasmid

    #60327

    Purpose

    Contains a promoter of two gal binding sites coupled with a core promoter derived from native Leucine promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Insert

    promoter
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pRS406-PHO5-GFP-CERT PH domain EE mutation

    Plasmid

    #58833

    Purpose

    Expresses GFP-Tagged and mutated CERT PH domain in yeast

    Depositor

    Tim Levine

    Article

    Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

    Insert

    collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
    

    Use

    Tags

    GFP and Myc

    Expression

    Yeast

    Mutation

    changed Arginine 43 to Glutamate

    Promoter

    PHO5

    Available since

    Aug. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pGLx2-set1deltaRRM

    Plasmid

    #53257

    Purpose

    GAL driven LexA-tagged SET1-deltaRRM (RRM domain deleted)

    Depositor

    Jean Cook

    Article

    Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

    Insert

    SET1
    

    Use

    Tags

    LexA

    Expression

    Yeast

    Mutation

    RRM domain deleted (aa 230-355)

    Promoter

    GAL1/10

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 413-Met25 Kar2 TorsinADE HDEL

    Plasmid

    #32118

    Depositor

    Susan Lindquist

    Article

    Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

    Insert

    TorsinA (TOR1A Human)
    

    Use

    Tags

    HDEL and Kar2

    Expression

    Yeast

    Mutation

    deletion of E at 302 or 303

    Promoter

    Available since

    Oct. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

24. p414GPD_Hsp82_CycTER

    Plasmid

    #61710

    Purpose

    Yeast plasmid with Hsp82

    Depositor

    Dan Bolon

    Article

    Jiang et al PLoS Genet. 2013 Jun;9(6):e1003600. doi:

    Insert

    6xHis-Hsp82 (HSP82 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRS424-TUB2 - human TUBB2a Cterminal tail

    Plasmid

    #60396

    Purpose

    Expression of chimeric yeast beta tubulin (TUB2) - human beta2 tubulin Cterminal tail (TUBB2a) using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    TUB2 amino acids 429 - end, replaced with human T…

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

26. pAG303GPD-ccdB-ECFP

    Plasmid

    #14158

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCGB42/Kan

    Plasmid

    #17261

    Depositor

    Stuart Schreiber

    Article

    Huang et al Proc Natl Acad Sci U S A. 1997 Dec 9. 94

    Type

    Empty backbone

    Use

    Tags

    B42•HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

28. pRB306

    Plasmid

    #8666

    Depositor

    David Botstein

    Article

    Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

29. pAG306GPD-ECFP-ccdB

    Plasmid

    #14284

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAG303GAL-ccdB-TAP

    Plasmid

    #14253

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 29, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. p416 3-F GW

    Plasmid

    #58552

    Purpose

    pRS416 carrying Gateway cassette for three inserts

    Depositor

    Leonid Kruglyak

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 24, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAG306GPD-Cerulean-ccdB

    Plasmid

    #14404

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Cerulean

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. YCplac111-KOG1 (pAN58)

    Plasmid

    #39209

    Depositor

    Michael Hall

    Article

    Loewith et al Mol Cell. 2002 Sep;10(3):457-68.

    Insert

    KOG1 (KOG1 Budding Yeast)
    

    Use

    Tags

    myc

    Expression

    Yeast

    Mutation

    L1512P

    Promoter

    KOG1

    Available since

    Dec. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSB819-PKR-gib-FFA

    Plasmid

    #20043

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2008 Nov 30. ():.

    Insert

    Protein kinase R
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Y489F

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

35. p413TEF-CARKL

    Plasmid

    #51747

    Purpose

    overexpression of mouse Sedoheptulokinase (CARKL, SHPK) in yeast

    Depositor

    Markus Ralser

    Article

    Kruger et al Antioxid Redox Signal. 2011 Jul 15;15(2)

    Insert

    SHPK (Shpk Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRB2070

    Plasmid

    #8671

    Depositor

    David Botstein

    Article

    Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

    Insert

    TUB1 with LYS2 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 30, 2005

    Availability

    Academic Institutions and Nonprofits only

37. pAG423GAL-ECFP-ccdB

    Plasmid

    #14293

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. M3939 leu2::ADE2 Disruptor Converter

    Plasmid

    #51672

    Purpose

    Yeast marker swap plasmid for converting LEU2 to ADE2

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    ADE2 (ADE2 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADE2

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p416GAL1-Ssk1t-L-IFP-F[1] (Yeast)

    Plasmid

    #52897

    Purpose

    Ssk1t replaces the zipper and is fused to the IFP-F[1] through the linker (GGGGS) X 2. Plasmid confers ampicillin resistance to DH5α.

    Depositor

    Stephen Michnick

    Article

    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

    Insert

    Ssk1t-L-IFP-F[1]
    

    Use

    Tags

    L-IFP-F[1]

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pDR-meptrac-H194E

    Plasmid

    #47764

    Purpose

    expresses MepTrac-H194E in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    MepTrac-H194E
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    H194E

    Promoter

    PMA

    Available since

    Sept. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pAG306GAL-ccdB-ECFP

    Plasmid

    #14163

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    ECFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pSB819-PKR-titi

    Plasmid

    #20035

    Depositor

    Harmit Malik

    Article

    Elde et al Nature. 2008 Nov 30. ():.

    Insert

    Protein kinase R
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

43. pRY1001

    Plasmid

    #21787

    Depositor

    Richard Yu

    Article

    Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

    Insert

    CFP-Ste12 (STE12 A. victoria, Budding Yeast)
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    contains 1000 bp UTR (Ste12 promoter) upstream of…

    Promoter

    Available since

    Oct. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. p413TEF-Sir2-P394L

    Plasmid

    #51744

    Purpose

    overexpression of Sir2 in S. cerevisiae (P394L mutant), HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    Sir2 (SIR2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    P394L

    Promoter

    TEF1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. LHP1468 - VPS23

    Plasmid

    #32239

    Depositor

    Linda Hicke

    Insert

    VPS23 (STP22 Budding Yeast)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    T7

    Available since

    Nov. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

46. 416Gal-FUS-R524S-YFP

    Plasmid

    #29626

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R524S

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

47. pDR-AmTrac-100

    Plasmid

    #47771

    Purpose

    expresses AmTrac-100 in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac-100
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    A141E, T464D

    Promoter

    PMA

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

48. pDR-meptrac

    Plasmid

    #47768

    Purpose

    expresses MepTrac in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    meptrac
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

49. pDR-AmTrac-IS

    Plasmid

    #47766

    Purpose

    expresses AmTrac-IS in yeast

    Depositor

    Wolf Frommer

    Article

    De Michele et al eLife. 2013 Jul 2;2:e00800.

    Insert

    AmTrac1;2
    

    Use

    Tags

    mcpGFP

    Expression

    Yeast

    Mutation

    Promoter

    PMA

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

50. 416Gal-FUS-R522G-YFP

    Plasmid

    #29625

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R522G

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only