8,350 results
-
Plasmid#112045PurposeCEN/ARS plasmid for GAL-inducible ORM1-V5-TEV-EGFP expression marked with URADepositorInsertORM1
TagsV5-TEV-EGFPExpressionYeastPromoterGAL1Available SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
PdarC1
Plasmid#24835DepositorInsertPdarC1
TagsHA and MycExpressionYeastAvailable SinceAug. 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_c8gRNA_RPR1t
Plasmid#64386Purposeencodes c8 gRNADepositorInsertc8 gRNA
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB3037(XI-5 MarkerFree)
Plasmid#73277PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-5 (Chr XI: 11779..118967)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
p416TEF-NQYFP
Plasmid#99552PurposeExpresses Swi1 NQ-YFP to monitor Swi1 state or induce [SWI+].DepositorAvailable SinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG413GAL-ccdB-DsRed
Plasmid#14357DepositorTypeEmpty backboneUseGateway CloningTagsDsRedExpressionYeastAvailable SinceMarch 29, 2007AvailabilityAcademic Institutions and Nonprofits only -
UKC1620-SUP35wt
Plasmid#29730DepositorInsertSUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
ExpressionYeastMutationWild type gene including genomic sequence 955 bp …Available SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSB819-PKR-hum-LFA
Plasmid#20051DepositorAvailable SinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yoEmerald-SpHis5
Plasmid#44851PurposeYeast tagging vector to create gene fusion with yeast optimized Emerald with SpHis5 selectionDepositorInsertyoEmerald
ExpressionYeastAvailable SinceMay 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG416GPD-ECFP-ccdB
Plasmid#14292DepositorTypeEmpty backboneUseGateway CloningTagsECFPExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ytdtomato-T2A-mTurquoise2
Plasmid#118451PurposeProduces equimolar expression of yeast codon-optimized tdTomato and mTq2DepositorInsertytdtomato-T2A-mTurquoise2
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRPR1_a1gRNA_RPR1t
Plasmid#64378Purposeencodes a1 gRNADepositorInserta1 gRNA
ExpressionYeastAvailable SinceMay 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKT0239
Plasmid#8738DepositorInsertyECitrine
Tags3x HA and Codon optimized linkerExpressionYeastMutationyEGFP with S65G, V68L, Q69M, S72A, T203YAvailable SinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAG415GPD-ccdB-ECFP
Plasmid#14170DepositorTypeEmpty backboneUseGateway CloningTagsECFPExpressionYeastAvailable SinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
-
pDRF'- AmTryoshka1;3-LS-F138I
Plasmid#100922PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-L255I
Plasmid#100964PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-F138I
Plasmid#100965PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-L255I
Plasmid#100966PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorAvailable SinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW eCFP-T2A-mCherry
Plasmid#118421PurposeProduces equimolar expression of eCFP and mCherryDepositorInserteCFP-T2A-mCherry
ExpressionYeastAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRBUBR1empty
Plasmid#24505DepositorTypeEmpty backboneExpressionYeastAvailable SinceApril 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
plp-strongSC_GFP
Plasmid#22312DepositorInsertGFP-rad9-GFP
UseIntegrating plasmidExpressionYeastAvailable SinceOct. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
-
pUK421
Plasmid#44534DepositorInsertGAL1-HHF2
ExpressionYeastPromoterGALAvailable SinceMay 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRS426-TUB1-internal6xHis (1-440)
Plasmid#60391PurposeExpression of yeast alpha tubulin (TUB1) - internal 6xHis without Cterminal tail using a GAL promoterDepositorInsertTUB1 (TUB1 Budding Yeast)
ExpressionYeastMutationinternal 6xHis (see supplement figure 4), amino a…PromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRS424-TUB2 (1-428)
Plasmid#60395PurposeExpression of yeast beta tubulin (TUB2) - without Cterminal tail using GAL promoterDepositorInsertTUB2
ExpressionYeastMutationdeleted 429 - endPromoterGALAvailable SinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
PP275
Plasmid#78968PurposePromoter: GAP, minimal Promoter: GCW14. Figure 4. Expresses GFP.DepositorInsertGFP
ExpressionYeastAvailable SinceDec. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA HDEL
Plasmid#32117DepositorAvailable SinceOct. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
Tags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastPromoterT7Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG424GPD-Cerulean-ccdB
Plasmid#14416DepositorTypeEmpty backboneUseGateway CloningTagsCeruleanExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJW3.42
Plasmid#62866PurposeYeast two-hybrid bait vector expressing LexA-PPTR-1 fusionDepositorInsertPPTR-1/pLexA-N bait
UseTwo-hybrid bait vectorTagsLexAExpressionYeastAvailable SinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Hsh155 K740R
Plasmid#111953PurposeThis is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the K740R mutationDepositorInsertHsh155 K740R
ExpressionBacterial and YeastMutationK740RAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWS936
Plasmid#123050PurposeG_ subunit for creating C-terminal transplantsDepositorInsertGpa1 C-terminal Truncation Vector [3-4a]
ExpressionYeastAvailable SinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS067
Plasmid#123044Purpose_-factor GPCRDepositorInsertSte2 [3]
ExpressionYeastAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS053
Plasmid#123025PurposePheromone responsive promoterDepositorInsertpFIG1 [2]
ExpressionYeastAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pWS060
Plasmid#123024PurposePheromone responsive promoterDepositorInsertpFUS1 [2]
ExpressionYeastAvailable SinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU2-RAD30cmyc
Plasmid#60821Purposeto overexpress yeast polymerase eta in yeastDepositorAvailable SinceDec. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGRE NPD-GFP
Plasmid#1197DepositorInsertN-terminus of Sup35 fused to GFP (SUP35 Budding Yeast)
TagsGFPExpressionYeastMutationfused N-terminal Sup35 (~1-253aa) to GFPAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
-
pAV113.loxP
Plasmid#63186PurposepRS413 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG. TU is also flanked by LoxP sites.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
p2AB42
Plasmid#17267DepositorTypeEmpty backboneTagsB42•HAExpressionYeastAvailable SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pNB785
Plasmid#60164PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by SIC1 promoter.DepositorInsertFLuc-yEVenus-PEST
TagsPEST degronExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB690
Plasmid#60134PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertYeLuc
TagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastPromoterMET17prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG9-GFP S122D
Plasmid#114667PurposeExpresses yeast ATG9 (S122D) under the endogenous promoter with a GFP C-terminal tagDepositorInsertATG9
TagsGFPExpressionYeastMutationchanged serine 122 to aspartic acidPromoterATG9Available SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS303-pGAL-HA-SrtAstrep (pKS78)
Plasmid#50029PurposeGalactose-inducible expression of cytosolic S. pyogenes SrtA in yeastDepositorInsertHA-SrtA(strep)
TagsHAExpressionYeastPromoterpGALAvailable SinceJan. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
M34 Xdsh PDZ bait-pGBT9
Plasmid#17190DepositorAvailable SinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pAG415GAL-ccdB-Cerulean
Plasmid#14385DepositorTypeEmpty backboneUseGateway CloningTagsCeruleanExpressionYeastAvailable SinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pCfB2801
Plasmid#63656PurposepCfB2801 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only