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8,173 results

Showing: 4001 - 4050 of 8173 results

  6. p41Neo 1-F GW

    Plasmid
    #58545
    Purpose
    CEN vector with G418 resistance marker for single Gateway insertion
    Depositor
    Leonid Kruglyak
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    58545-plasmid-map-sequence-id-90632

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  8. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

    Plasmid
    #60393
    Purpose
    Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)
    Depositor
    Ron Vale
    Article
    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do
    Insert
    TUB1 (TUB1 Synthetic, Budding Yeast)
    Expression
    Yeast
    Mutation
    internal 6xHis (see supplement figure 4)
    Promoter
    GAL
    Available Since
    Jan. 26, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pAAH3

    Plasmid
    #41827
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for phleomycin resistance.
    Depositor
    Melissa Moore
    Article
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Yeast
    Promoter
    TEF
    Available Since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41827-plasmid-map-sequence-id-69168

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  19. pNB787

    Plasmid
    #60152
    Purpose
    Firefly luciferase (Promega) and yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by LEU1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    LEU1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60152-plasmid-map-sequence-id-96317

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  22. pCfB4780

    Plasmid
    #106143
    Purpose
    EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntD_1, USER site for cloning, amp resistance
    Depositor
    Irina Borodina
    Article
    Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/
    Type
    Empty backbone
    Expression
    Yeast
    Available Since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106143-plasmid-map-sequence-id-205317

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  26. pJT3

    Plasmid
    #98304
    Purpose
    Overexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of CUP1 promoter
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    TRPL3AcNES1>TRPL41B
    Expression
    Yeast
    Available Since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    98304-plasmid-map-sequence-id-190175

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  30. pUDP010

    Plasmid
    #101166
    Purpose
    E. coli/S. cerevisiae amdS shuttle vector expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 in S. pastorianus (HH-gRNASeILV6-HDV)
    Depositor
    Jean-Marc Daran
    Article
    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.
    Insert
    HH-gRNA-HDV targetting SeILV6 in S. pastorianus
    Use
    CRISPR
    Expression
    Yeast
    Promoter
    ScTDH3
    Available Since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101166-plasmid-map-sequence-id-199274

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  31. pREP4xV5_ccdB

    Plasmid
    #79016
    Purpose
    Gateway destination vector for S. pombe. Allow sequence of interest to be fused to N-terminal double V5 epitope tag. attR1-CmR-ccdB-attR2 cassette, nmt1 (ATATATAAA) promoter, ura4+ selection
    Depositor
    Göran Akusjärvi, Pernilla Bjerling
    Type
    Empty backbone
    Tags
    double V5 epitope tag
    Expression
    Yeast
    Available Since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    79016-plasmid-map-sequence-id-147773

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  40. pNB789

    Plasmid
    #60166
    Purpose
    Firefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion with PEST degron driven by RNR1 promoter.
    Depositor
    Nicolas Buchler
    Article
    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14
    Insert
    FLuc-yEVenus-PEST
    Tags
    PEST degron
    Expression
    Yeast
    Mutation
    FLuc has A4V & S504G (no functional effect)
    Promoter
    RNR1pr
    Available Since
    March 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60166-plasmid-map-sequence-id-96375

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  50. GTL-ir

    Plasmid
    #81102
    Purpose
    Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Mutation
    codon optimized second iRFP to avoid internal rec…
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81102-plasmid-map-sequence-id-154289

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Showing: 4001 - 4050 of 8173 results