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pNX3a-FL3

Plasmid

#78358

Purpose

fission yeast epitope tagging

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xFLAG
kanMX6

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

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piHGpd/G170D

Plasmid

#1113

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

HSP82 with glycine 170 changed to aspartic acid; …

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pJJH1338

Plasmid

#36912

Depositor

Jürgen Heinisch

Article

Bakota et al Mol Genet Genomics. 2012 Apr;287(4):313-

Insert

ScURA3

Use

Lentiviral

Tags

Expression

Bacterial, Mammalian, and Y…

Mutation

Promoter

Available since

July 17, 2012

Availability

Academic Institutions and Nonprofits only

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pAG415GPD-ccdB-Cerulean

Plasmid

#14386

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

Cerulean

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pXP832

Plasmid

#46066

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pXP811

Plasmid

#46061

Depositor

Nancy DaSilva

Article

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

PGK1

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCuGFP-SpAtg17(416)

Plasmid

#122080

Purpose

Expressing S. pombe GFP-Atg17

Depositor

Daniel Klionsky

Article

Nanji et al Autophagy. 2017;13(12):2018-2027. doi: 1

Insert

SpATG17 (atg17 Fission Yeast)

Use

Tags

GFP

Expression

Yeast

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAG425GPD-ccdB-EYFP

Plasmid

#14226

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pWS939

Plasmid

#123051

Purpose

Pheromone responsive transcription factor

Depositor

Tom Ellis

Article

Shaw et al Cell. 2019 Apr 18;177(3):782-796.e27. do

Insert

Ste12 [3]

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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sub2-1 pRS315

Plasmid

#111301

Purpose

sub2-1 in pRS315

Depositor

Christine Guthrie

Article

Kistler et al Genes Dev. 2001 Jan 1;15(1):42-9.

Insert

SUB2

Use

Tags

Expression

Yeast

Mutation

sub2-1

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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PP165

Plasmid

#78985

Purpose

Supplemental Figure 4. Promoter GCW14. Expresses GFP.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pRS416-PHO5-GFP-2xOsh2 PH domain EE mutation

Plasmid

#58830

Purpose

Expresses GFP-Tagged and mutated Osh2 PH domain in yeast

Depositor

Tim Levine

Article

Roy et al J Biol Chem. 2004 Oct 22;279(43):44683-9

Insert

2 x Osh2 PH domain (OSH2 Budding Yeast)

Use

Tags

GFP and Myc

Expression

Yeast

Mutation

changed Lysine 307 to Glutamate and Arginine 309 …

Promoter

PHO5

Available since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
pTH651-RLuc/minFLuc

Plasmid

#29697

Depositor

Tobias von der Haar

Article

Chu et al Nucleic Acids Res. 2011 May 9. ():.

Insert

Firefly Luciferase

Use

Tags

Expression

Yeast

Mutation

All codons of the original FLuc gene were exchang…

Promoter

Available since

Aug. 25, 2011

Availability

Academic Institutions and Nonprofits only

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Ube2t

Plasmid

#31427

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2T Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pAG305GPD-EGFP-ccdB

Plasmid

#14306

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET-T2A-mTurquoise2

Plasmid

#118444

Purpose

Produces equimolar expression of ymYPET and mTq2

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET-T2A-mTurquoise2

Use

Tags

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pAG425GAL-ccdB-EYFP

Plasmid

#14225

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
Ube2d3

Plasmid

#31411

Depositor

Rachel Klevit

Article

Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

Insert

Ubiquitin Conjugating Enzyme (UBE2D3 Human)

Use

Tags

HA

Expression

Yeast

Mutation

Promoter

Available since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only
pKT0221

Plasmid

#8726

Depositor

Kurt Thorn

Article

Sheff et al Yeast 2004 Jun;21(8):661-70.

Insert

yECFP

Use

Tags

3HA and Codon optimized linker

Expression

Yeast

Mutation

yEGFP with F64L, S65T, Y66W, N146I, M153T, V163A

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only

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LD223

Plasmid

#69190

Purpose

Integration of 2GFP tag at Atg23 C terminus, use auxotrophic marker URA3(Candida albicans)

Depositor

Zhiping Xie

Article

Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

Insert

Atg23-2EGFP

Use

Tags

2xEGFP

Expression

Yeast

Mutation

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAV0762

Plasmid

#133496

Purpose

Expression of nucleo-cytoplasmic mCherry in M-cells

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(mam1*)-mCherry-terminator(ScADH1)-kanMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pNXRVd-HACre

Plasmid

#78404

Purpose

constitutive Cre expression vector

Depositor

Kazunori Tomita

Article

Amelina et al BMC Biotechnol. 2016 Nov 8;16(1):76.

Inserts

3xHA-nls-Cre
ura4+ (ura4 Fission Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGADT7 Hsh155 Y292C

Plasmid

#87291

Purpose

Encodes Y2H Gal4 activation domain for Hsh155 Y292C

Depositor

Aaron Hoskins

Article

Carrocci et al Nucleic Acids Res. 2017 Jan 6. pii: gkw1

Insert

HSH155 (HSH155 Budding Yeast)

Use

Tags

GAL4 AD and HA

Expression

Bacterial and Yeast

Mutation

Y292C

Promoter

Available since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG425GAL-EYFP-ccdB

Plasmid

#14345

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pURD214

Plasmid

#113871

Purpose

expression of Cas9 programming sgRNA5 and sgRNA2 targetting HXT13-15-16 and HXT2 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA5 HXT13-15-16 sgRNA2-HXT2

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDR211

Plasmid

#113870

Purpose

expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA3-HXT8 / sgRNA4-HXT14

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDR220

Plasmid

#113873

Purpose

expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA8-HXT10 sgRNA7-HXT9-11-12

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDR418

Plasmid

#113875

Purpose

expression of double Cas9 programming sgRNA11 targetting STL1

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

doble sgRNA11-STL1

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pUDR295

Plasmid

#113874

Purpose

expression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA2 GAL2 sgRNA1-HXT4-1-5;HXT3-6-7

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNC1053

Plasmid

#20406

Depositor

Beverly Errede

Article

Hackett et al Yeast. 2006 Apr 15. 23(5):333-49.

Insert

CFP

Use

Tags

Expression

Yeast

Mutation

linker: delta k

Promoter

Available since

March 6, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG414GPD-EYFP-ccdB

Plasmid

#14336

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EYFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-PRP8

Plasmid

#89966

Purpose

complements prp8 genomic deletion

Depositor

Christine Guthrie

Article

Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

none

Promoter

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS313-PRP8

Plasmid

#90964

Purpose

yeast vector

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

Insert

PRP8 (PRP8 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

native

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Osa-pCAMBIA-1301-STTM396

Plasmid

#84206

Purpose

Disturb the expression of miR396 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR396

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pMJA151

Plasmid

#128526

Purpose

Expresses mutant Ber e 1 in Pichia

Depositor

Marcos Alcocer

Insert

mutant Ber e 1

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
720-2µ-maxHIS3

Plasmid

#40612

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Synthetic, Budding Yeast)

Use

Tags

HA

Expression

Yeast

Mutation

Every codon of the coding sequence has been repla…

Promoter

TDH3

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pXP116

Plasmid

#26813

Depositor

Nancy DaSilva, Suzanne Sandmeyer

Article

Fang et al Yeast. 2010 Sep 10. ():.

Type

Empty backbone

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Dec. 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pK3F-PYK1

Plasmid

#85771

Purpose

N-ICE plasmid pK3F-PYK1

Depositor

Scott Briggs

Article

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-KanMX-loxP-PYK1p-3FLAG

Use

Cre/Lox

Tags

Expression

Yeast

Mutation

Promoter

Available since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415GPD-ECFP-ccdB

Plasmid

#14290

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

ECFP

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLx2-SET1

Plasmid

#53256

Purpose

GAL driven LexA-tagged SET1

Depositor

Jean Cook

Article

Rizzardi et al Genetics. 2012 Oct;192(2):371-84. doi: 1

Insert

SET1

Use

Tags

LexA

Expression

Yeast

Mutation

Promoter

GAL1/10

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge

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