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We narrowed to 69 results for: Tfg

Showing: 1 - 40 of 69 results

  1. pTFG005_Spike_Inducible_3xFlag

    Plasmid
    #191578
    Purpose
    Inducible expression of SARS-CoV-2 Spike protein with C-terminal 3x FLAG tag under TRE3G promoter and cloned into a 2nd generation lentiviral transfer plasmid (modified pLVX with Hygro resistance).
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Lentiviral
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    191578-plasmid-map-sequence-id-442483

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  2. pTFG011_Spike_Parental_D614G_3xFlag

    Plasmid
    #191571
    Purpose
    SARS-CoV-2 Spike protein under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G mutation. Last 19 amino acids truncated.
    Promoter
    CMV
    Available since
    July 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    191571-plasmid-map-sequence-id-440477

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  4. pTFG027_Spike_sACE2-resistant_D614G_F486S_3xFlag

    Plasmid
    #191572
    Purpose
    Mutated SARS-CoV-2 Spike under CMV with D614G, C-term 19 amino acid truncation, and 3x FLAG. Encodes F486S mutation. For expression and pseudotyping lentiviral particles.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    SARS-CoV-2 Spike Protein (S Synthetic)
    Use
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    D614G and F486S (confers reduced binding affinity…
    Promoter
    CMV
    Available since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191572-plasmid-map-sequence-id-441975

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  5. pTFG001-ACE2(WT)-HA

    Plasmid
    #191569
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(WT)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA tag.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon optimized for expression in human cells
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191569-plasmid-map-sequence-id-441362

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  9. pTFG002-ACE2(Mut)-HA

    Plasmid
    #191570
    Purpose
    ACE2 insert in a 2nd generation lentiviral transfer plasmid (pGIPZ) to generate ACE2(Mut)-expressing cells. Codon-optimized for human expression. Insert contains C-terminal HA Tag.
    Depositor
    Joshua Leonard
    Article
    Gunnels et al Small. 2022 May;18(19):e2200125. doi: 10
    Insert
    Angiotensin Converting Enzyme II (ACE2 Human)
    Use
    Lentiviral and RNAi
    Tags
    HA tag
    Expression
    Mammalian
    Mutation
    Codon-optimized for human expression. Also contai…
    Promoter
    CMV
    Available since
    Aug. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    191570-plasmid-map-sequence-id-441411

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  14. pBabepuro-TGFA

    Plasmid
    #189610
    Purpose
    Retroviral expression vector for TGFA
    Depositor
    Thierry Tchenio
    Insert
    TGFA (TGFA Human)
    Use
    Retroviral
    Tags
    Expression
    Mutation
    Promoter
    SV40 early promoter
    Available since
    Aug. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    189610-plasmid-map-sequence-id-381090

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  16. FUGW-APPwt-T2A-mCherry

    Plasmid
    #190803
    Purpose
    Express wild-type APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Ubi C
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    190803-plasmid-map-sequence-id-374464

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  17. pET-Sac-Abeta(MC1-40)

    Plasmid
    #127152
    Purpose
    Expresses N-terminal cysteine Abeta(1-40)
    Depositor
    James Nowick
    Insert
    Abeta(MC1-40) (APP Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    127152-plasmid-map-sequence-id-247836

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  18. FUGW-APPSwe-T2A-Hygro

    Plasmid
    #190805
    Purpose
    Express Swedish mutant APP from Ubiquitin C promoter, co-expressing Hygro resistance gene
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Swedish (KM670/671NL)
    Promoter
    Ubi C
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190805-plasmid-map-sequence-id-372847

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  19. FUGW-APPIce-T2A-mCherry

    Plasmid
    #190806
    Purpose
    Express Icelandic mutant APP from Ubiquitin C promoter, co-expressing mCherry
    Depositor
    Ronald Hart
    Insert
    APP (APP Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Icelandic (A673T)
    Promoter
    Ubi C
    Available since
    Sept. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    190806-plasmid-map-sequence-id-372850

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  21. pc DNA FRT TO- APP695

    Plasmid
    #114193
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    wild type splice variant 695: 695AA (2085bp, lack…
    Promoter
    CMV
    Available since
    Oct. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    114193-plasmid-map-sequence-id-224265

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  22. pAG426-GAL-APP-CTF

    Plasmid
    #181732
    Purpose
    Galactose inducible expression of APP-CTF
    Depositor
    Alejandro Chavez
    Insert
    APP-CTF (APP Human)
    Use
    Tags
    Expression
    Yeast
    Mutation
    C-terminal fragment (aa 671-770)
    Promoter
    Available since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    181732-plasmid-map-sequence-id-437192

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  23. His-FGE

    Plasmid
    #75147
    Purpose
    Expression vector for His(6) tagged Formylglycine Generating Enzyme (FGE) in bacterial host.
    Depositor
    Richard Fishel
    Article
    Liu et al Sci Rep. 2015 Nov 19;5:16883. doi: 10.10
    Insert
    FGE
    Use
    Tags
    Histidine tag and Thrombin
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    T7
    Available since
    July 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. pc DNA FRT TO- APPSwed/Ind

    Plasmid
    #114194
    Purpose
    Creation of a stable cell line expressing APP mutation (AD in vitro study)
    Depositor
    Aleksandra Radenovic
    Insert
    Amyloid-Precursor-Protein (APP Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Popular mouse model, overexpress 2 mutations: Swe…
    Promoter
    CMV
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114194-plasmid-map-sequence-id-224256

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  26. pET28a-TGFα-GSY-SPM

    Plasmid
    #163615
    Purpose
    Expresses mature human Transforming Growth Factor alpha fused to SPM in bacterial cells for calcium-induced covalent coupling to Epidermal Growth Factor Receptor.
    Depositor
    Mark Howarth
    Article
    Scheu et al Nat Commun. 2021 Jan 29;12(1):717. doi:
    Insert
    TGFa-GSY-SPM (TGFA Human, Synthetic)
    Use
    Tags
    C-tag and His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    163615-plasmid-map-sequence-id-323634

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  31. TFORF3420

    Plasmid
    #144896
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SNAPC1 (SNAPC1 Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Aug. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    144896-plasmid-map-sequence-id-404466

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Showing: 1 - 40 of 69 results